Exchange of signal molecules derived from fungal parasites and plants on the plant surfaces and then-signal transmission leading to defense reaction or accessibility induction.

2005 Fiscal Year Final Research Report Summary

• Project/Area Number: 12052219
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Okayama University
• Principal Investigator: SHIRAISHI Tomonori Okayama Univ., Faculty of Agriculture, Prof., 農学部, 教授 (10033268)
• Co-Investigator(Kenkyū-buntansha): KUBO Yasuyuki Kyoto Pref.Univ., Faculty of Agriculture, Prof., 農学部, 教授 (80183797) ; KUNOH Hitoshi Mie Univ., Emer., Prof., 名誉教授 (20024573)
• Project Period (FY): 2000 – 2005
• Keywords: Qctadecanoic acid / AtMT method / Osmotin-resistant gene / Arabidopsis thaliana ecotype In-0 / Extracellular matrix / Esterase / NTPase-transformd tobacco / Virus-inducing gene silencing

Research Abstract

In this project, we found that, 1) A new inducer for germination of conidiospores of a fungal pathogen (Colktotrichum lagenarium) is a derivative of octadecanoic acid ; 2) A gene for resistance to osmotin-like proteins was cloned as s new virulent gene from C.lagenarium by AtMT method. Disruption of this ortholog in Magnaporthe grisea resulted in avirulent to rice plant ; 3) In-0, an ecotype of Arabidopsis thaliana showed resistance to C.higginsianum. This resistance gene is located the 4th chromosome with several molecular markers ; 4) Conidiospores of an incompatible powdery mildew fungus (Erysiphe pisi) secreted immediately extracellular matrix (ECM) including esterase on the surface of plants when these spores contacted. This ECM induced inaccessibility (resistance at cell level) to the compatible challenger ; 5) Cell wall-bound NTPase is a receptor for exogenous signal molecules. Inorganic phosphate, one of the products by NTPases induced defense response in plants. Transgenic tobacco with NTPase gene showed resistance to virulent fungi ; 6) VTGS of NTPase in Nicotiana benthamiana showed susceptibility to bacterial pathogens, indicating that NTPases are lightly associated with nonhost resistance.

Research Products

• [Journal Article] Identification of the genes expressed during spore germination of Mycosphaerella pinodes. (2005)
  • Author(s): Takahara, H., Toyoda, K., Tsuji, G., Kubo, Y., Ichinose, Y., Shiraishi, T.
  • Journal Title: Journal of General Plant Pathology. 71 Pages: 190-195
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Identification of the genes expressed during spore germination of Mycosphaerella pinodes. (2005)
  • Author(s): Takahara, H., Toyoda, K., Tsuji, G., Kubo, Y., Ichinose, Y., Shiraishi, T.
  • Journal Title: J.Gen.Plant Pathol 71 Pages: 190-195
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Agrobacterium tumefaciens-mediated transformation as a tool for random mutagenesis of Colletotrichum trifolii. (2004)
  • Author(s): Takahara, H., Tsuji, G., Kubo, Y., Yamamoto M., Toyoda, K., Shiraishi, T.
  • Journal Title: Journal of General Plant Pathology. 70 Pages: 93-96
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] RCHI, a locus in Arabidopsis that confers resistance to the hemibiotrophic fungal pathogen Colletotrichum higginsianum. (2004)
  • Author(s): Narusaka, Y., Narusaka, M., Park, P., Kubo, Y.et al.
  • Journal Title: Molecular Plant-Microbe Interactions 17 Pages: 749-762
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Rapid pre-germination and germination responses of Erysiphe pisi conidia to contact and light. (2004)
  • Author(s): Fujita, K., Wright, A.J., Meguro, A., Kunoh H., Carver, T.L.W.
  • Journal Title: Journal of General Plant Pathology. 70 Pages: 75-84
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Increased inaccessibility induced in barley cells after exposure to extracellular material released by nonpathogenic powdery mildew conidia. (2004)
  • Author(s): Fujita, K., Suzuki, T., Kunoh, H., Carver, T.L.W., Thomas, B.J., Gurr, S.J.
  • Journal Title: Physiological and Molecular Plant Patholology 64 Pages: 169-178
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Differential regulation of MBP kinases by a glycoprotein elicitor and a polypeptide suppressor from Mycosphaerella pinodes in pea. (2004)
  • Author(s): Uppalapati, S.R., Toyoda, K., Ishiga, Y., Ichinose, Y., Shiraishi, T.
  • Journal Title: Physiological and Molecular Plant Pathology 64 Pages: 17-25
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Agrobacterium tumefaciens-mediated transformation as a tool for random mutagenesis Of Colletotrichum trifolii (2004)
  • Author(s): Takahara, H., Tsuji, G., Kubo, Y., Yamamoto, M., Toyoda, K., -, Shiraishi, T.
  • Journal Title: Journal of General Plant Pathology 70 Pages: 93-96
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] RCHI, a locus in Arabidopsis that confers resistance to the hemibiotrophic fungal pathogen Colletotricum higginsianum. (2004)
  • Author(s): Narusaka, Y., Narusaka, M., Park, P., Kubo, Y., Hirayama, T., Seki, M., Shiraishi.T., Ishida, J., Nakashima, M., Enju, A., Sakurai, T., Satou, M., Kobayashi, M., Shinozaki, K.
  • Journal Title: Mol.Plant-Microbe Interactions 17 Pages: 749-762
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Rapid pre-germination and germination responses of Erysiphe pisi conidia to contact and light. (2004)
  • Author(s): Fujita, K., Wright, A.J., Meguro, A., Kunoh H., Carver, T.L.W.
  • Journal Title: Journal of General Plant Pathology 70 Pages: 75-84
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Differential regulation of MBP kinases by a glycoprotein elicitor and a polypeptide suppressor from Mycosphaerella pinodes in pea. (2004)
  • Author(s): Uppalapati, S.R., Toyoda, K., Ishiga, Y., Ichinose, Y., Shiraishi.T.
  • Journal Title: Physiological and Molecular Plant Pathology 64 Pages: 17-25
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Genomic and Genetic Analysis of plant Parasitism and defense. (2004)
  • Author(s): Shiraishi, T., Toyoda, K.et al.
  • Journal Title: American Phythopathol.Soc. (Press) Pages: 301
  • Description: 「研究成果報告書概要(欧文)」より
• [Book] Genomic and Genetic Analysis of Plant Parasitism and Defense (Tsuyumu et al. eds.) (2004)
  • Author(s): Shiraishi, T., Toyoda, K., et al.
  • Total Pages: 301
  • Publisher: American Phytopathlical Society Press
  • Description: 「研究成果報告書概要(和文)」より
• [Patent(Industrial Property Rights)] 植物病害防除剤 (2001)
  • Inventor(s): 久能 均他5名
  • Industrial Property Rights Holder: 久能 均, 赤塚植物
  • Industrial Property Number: 特許第3629212号
  • Filing Date: 2001-01-17
  • Acquisition Date: 2004-12-17
  • Description: 「研究成果報告書概要(和文)」より
• [Patent(Industrial Property Rights)] Plant Disease Control Agent (2001)
  • Inventor(s): Kunoh, H. 他5名
  • Industrial Property Rights Holder: Kunoh, H., Aka
  • Industrial Property Number: US 6, 544, 511 B2
  • Filing Date: 2001-03-09
  • Acquisition Date: 2003-04-08
  • Description: 「研究成果報告書概要(和文)」より