2002 Fiscal Year Annual Research Report

新しい生理活性複合糖質の発見と機能に関する基礎的研究

Research Project

Project/Area Number	12306007
Research Institution	Gifu University
Principal Investigator	木曽真岐阜大学, 農学部, 教授 (90092931)
Co-Investigator(Kenkyū-buntansha)	安藤進東京都老人総合研究所, 生体膜部門, 副部長 (30073000) 神奈木玲児愛知県がんセンター, 病理学第二部, 部長 (80161389) 石田秀治岐阜大学, 農学部, 助教授 (20203002)
Keywords	セレクチン / シアリルルイスX / ガングリオシド / シアル酸 / シアロ糖鎖
Research Abstract	(1)L-セレクチンの内在性リガンドであるシアリル6-スルホルイスX構造を含むGlyCAM-1のO-グリコシド型9糖糖鎖の効率的合成にはじめて成功した(Aust. J. Chem.,55,105-112,2002)。また、創薬的見地から、シアリルルイスX関連糖脂質をセレクチンリガンドとし、P-およびL-セレクチンに対するチロシンサルフェイト誘導体の協力効果について検討した(Immunology,105,350-359,2002)。(2)サイクリック(ラクタム型)シアル酸を含むガングリオシドGM4をモデル化合物として、L-セレクチンの新規リガンド構造のNMR解析を行った(Magn. Reson. Chem.,40,517-523,2002)。(3)O-グリカンにシアル酸を転位するα2,8-sialyltransferase(ST8SiaVI)のクローニング及びその発現様式を解明した(J. Biol. Chem.,277,24030-24038)。(4)糖鎖-糖鎖相互作用によって引き起こされるGM3ガングリオシドの選択的分子反応の物理化学的解析を行った(Langmuir,18,6940-6945,2002)。(5)デ-N-アセチルシアリルルイスXガングリオシドの合成と同族分子種のL-セレクチン結合活性を詳細に検討し、リガンド活性の順位を決定した(Carbohydr. Res.,337,2111-2117,2002)。(6)生理活性シアロ糖鎖の構造と機能に関する化学生物学的研究をレビユーした(日本農芸化学会誌、1158-1167、2002)。

Research Products

(12 results)

All Other

All Publications (12 results)

[Publications] N.Otsubo: "Synthetic Studies on Sialoglycoconjugates. CXXIV. Total Synthesis of O-Glycan on the L-Selectin Ligand GlyCAM-1"Aust. J. Chem.. 55. 105-112 (2002)
[Publications] K.Brandenburg: "Biophysical characterization of triacyl monosaccharide lipid A partial structures in relation to bioactivity"Biophysical J.. 83. 322-333 (2002)
[Publications] R.L.Schnaar: "Immunoglobulin G-Class mouse monoclonal antibodies to major brain gangliosides"Analytical Biochem.. 302. 276-284 (2002)
[Publications] S.Takashima: "Molecular cloning and expression of a sixth type of α2,8-sialyltransferase (ST8Sia VI) that sialylates O-glycans"J. Biol. Chem.. 277. 24030-24038 (2002)
[Publications] T.Hamada: "NMR analysis of novel ganglioside GM4 analogues containing de-N-acetyl and lactamized sialic acid : probes for searching new ligand structures for human L-selectin"Magn. Reson. Chem.. 40. 517-523 (2002)
[Publications] C.Galustian: "Synergistic interactions of the two classes of ligand, sialyl-Lewis-<a/x> fuco-oligosaccharides and short sulpho-motifs, with the P-and L-selectins : implications for therapeutic inhibitor designs"Immunology. 105. 350-359 (2002)
[Publications] N.Otsubo: "An efficient and straightforward syntheses of sialyl Le^x glycolipid as a potent selectin blocker"J. Carbohydr. Chem.. 21. 247-255 (2002)
[Publications] K.Matsuura: "Selective Expansion of GM3 Glycolipid Monolayer induced by Carbohydrate-carbohydrate Interaction with Gg3 Trisaccharide-bearing Glycoconjugate Polystyrene at the Air-water Interface"Langmuir. 18. 6940-6945 (2002)
[Publications] K.Fukunaga: "Synthesis of sialyl-a-(2-3)-neolactotetraose derivatives modified at C-2 of the N-acetylglucosamine residue : probes for investigation of acceptor specificity of human a-1,3-fucosyltransferases, Fuc-TVII and Fuc-TVII."J. Carbohydr. Chem.. 21. 385-409 (2002)
[Publications] 木曽真: "生理活性シアロ糖鎖の構造と機能に関する化学生物学的研究"日本農芸化学会誌. 76・12. 1158-1167 (2002)
[Publications] T.Komori: "A first total synthesis of a novel sulfated ganglioside, 3'-O-sulfo-GM1b"Carbohydr. Res.. 337. 1679-1686 (2002)
[Publications] M.Yamaguchi: "Synthesis and selectin-binding activity of N-deacetylsialyl Lewis X ganglioside"Carbohydr. Res.. 337. 2111-2117 (2002)

2002 Fiscal Year Annual Research Report

新しい生理活性複合糖質の発見と機能に関する基礎的研究

Principal Investigator

木曽 真 岐阜大学, 農学部, 教授 (90092931)

Research Products

[Publications] N.Otsubo: "Synthetic Studies on Sialoglycoconjugates. CXXIV. Total Synthesis of O-Glycan on the L-Selectin Ligand GlyCAM-1"Aust. J. Chem.. 55. 105-112 (2002)

[Publications] K.Brandenburg: "Biophysical characterization of triacyl monosaccharide lipid A partial structures in relation to bioactivity"Biophysical J.. 83. 322-333 (2002)

[Publications] R.L.Schnaar: "Immunoglobulin G-Class mouse monoclonal antibodies to major brain gangliosides"Analytical Biochem.. 302. 276-284 (2002)

[Publications] S.Takashima: "Molecular cloning and expression of a sixth type of α2,8-sialyltransferase (ST8Sia VI) that sialylates O-glycans"J. Biol. Chem.. 277. 24030-24038 (2002)

[Publications] T.Hamada: "NMR analysis of novel ganglioside GM4 analogues containing de-N-acetyl and lactamized sialic acid : probes for searching new ligand structures for human L-selectin"Magn. Reson. Chem.. 40. 517-523 (2002)

[Publications] C.Galustian: "Synergistic interactions of the two classes of ligand, sialyl-Lewis-<a/x> fuco-oligosaccharides and short sulpho-motifs, with the P-and L-selectins : implications for therapeutic inhibitor designs"Immunology. 105. 350-359 (2002)

[Publications] N.Otsubo: "An efficient and straightforward syntheses of sialyl Le^x glycolipid as a potent selectin blocker"J. Carbohydr. Chem.. 21. 247-255 (2002)

[Publications] K.Matsuura: "Selective Expansion of GM3 Glycolipid Monolayer induced by Carbohydrate-carbohydrate Interaction with Gg3 Trisaccharide-bearing Glycoconjugate Polystyrene at the Air-water Interface"Langmuir. 18. 6940-6945 (2002)

[Publications] K.Fukunaga: "Synthesis of sialyl-a-(2-3)-neolactotetraose derivatives modified at C-2 of the N-acetylglucosamine residue : probes for investigation of acceptor specificity of human a-1,3-fucosyltransferases, Fuc-TVII and Fuc-TVII."J. Carbohydr. Chem.. 21. 385-409 (2002)

[Publications] 木曽 真: "生理活性シアロ糖鎖の構造と機能に関する化学生物学的研究"日本農芸化学会誌. 76・12. 1158-1167 (2002)

[Publications] T.Komori: "A first total synthesis of a novel sulfated ganglioside, 3'-O-sulfo-GM1b"Carbohydr. Res.. 337. 1679-1686 (2002)

[Publications] M.Yamaguchi: "Synthesis and selectin-binding activity of N-deacetylsialyl Lewis X ganglioside"Carbohydr. Res.. 337. 2111-2117 (2002)

木曽真岐阜大学, 農学部, 教授 (90092931)

[Publications] 木曽真: "生理活性シアロ糖鎖の構造と機能に関する化学生物学的研究"日本農芸化学会誌. 76・12. 1158-1167 (2002)