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2002 Fiscal Year Final Research Report Summary

Development of Dynamic Sensing System to Detect Environmental Stresses Induced in Living Cell. - Application to Diagnosis of Living Cell

Research Project

Project/Area Number 12450093
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Dynamics/Control
Research InstitutionYamagata University

Principal Investigator

KOSAWADA Tadashi  Yamagata University, Mechanical Systems Engineering, Associate Professor, 工学部, 助教授 (10143083)

Co-Investigator(Kenkyū-buntansha) IIJIMA Yoshiaki  Yamagata University, School of Medicine. Research Associate, 医学部, 助手
KUBOTA Yohko  Yamagata University, School of Medicine, Associate Professor, 医学部, 助教授 (60125763)
Project Period (FY) 2000 – 2002
KeywordsLiving cell / Environmental stress / Dynamic property / Micro sensor / Vibration / Diagnosis / Environmental hormone / Cultured cartilage
Research Abstract

In this study, a novel method to detect the environmental stresses induced in cell-like minute object is presented by using dynamic response of piezoelectric vibrator. Experimental studies have shown its capability to detect changes of the environmental stresses induced in egg cells of killifish as well as hardening process of tissue-engineered 3D cultured cartilage. The results can be summarized as follows.
(1) A novel method of sensing to detect the softness of sponge-like soft and cell-like minute object is specifically presented by using dynamic response of piezoelectric vibrator. Experimental studies conducted on silicone rubber blocks, whose Shore hardness, Hs are known as 5-80, have shown the sensor's capability to classify the softness of sponge-like soft object.
(2) With the aid of this sensing system, it is found that the living egg cells of killifish reveal their specific responses to the environments such as changes of temperature, concentration, pH, environmental hormone, etc. of surrounding solution or sucking pressure applied to the outside surface of the cell. Thus, the present sensor system has enough capability to monitor the environmental stresses induced in living cells.
(3) The hardening process of tissue-engineered three dimensionally (3D) cultured cartilage has been studied with respect to culture periods. Dynamic properties as well as biochemical composition were examined. It is found that the present study has a possibility to provide a useful information to decide whether the cultured cartilage has enough hardness for transplantation treatment of articular cartilage damage.

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] 小沢田, 佐藤, 山下: "細胞の力学特性検知システムの開発"日本機械学会Dynamics and Design Conf.2000 CD-ROM論文集. No.726. 6 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Kosawada, H.Satoh, H.Nishida: "Development of a Softness Sensing System by Using Dynamic Response of Piezoelectric Vibrator and Its Application to Detect Mechanical Properties of Living Cells"JSME International Journal, Series C. Vol.43, No.4. 772-779 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 小沢田, 佐藤, 山下: "卵細胞の環境ストレス検知システムの開発"日本機械学会バイオエンジニアリング講演会論文集. No.00-35. 286-287 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Kosawada, H.Satoh, T.Yamashita: "Piezo Micro Probe Sensor System to Detect Environmental Stresses Induced in Living Cells"Proceedings of Joint Symposium on Bio-Sensing and Bio-Imaging, Yamagata. 24 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 小沢田, 山村, 今野, 金沢: "3次元微小生体組織の力学特性検知システム"日本機械学会2002年次大会講演論文集. Vol.1, No.02-1. 109-110 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 小沢田, 今野, 山村, 金沢: "ピエゾマイクロ振動センサーを用いる3次元微小細胞組織の力学特性検知システム"日本機械学会東北支部第38期総会・講演論文集. No.031-1. 180-181 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Kosawada, H.Satoh, T.Yamashita: "Development of Sensing System for Dynamic Characteristics of Living Cells"Proceedings of the JSME Dynamics & Design Conference 2000. No.726. 6 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Kosawada, H.Satoh, H.Nishida: "Development of a Softness Sensing System by Using Dynamic Response of Piezoelectric Vibrator and Its Application to Detect Mechanical Properties of Living Cells"JSME International Journal, Series C. Vol.43,No.4. 772-779 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Kosawada, H.Satoh, T.Yamashita: "Development of Sensing System for Environmentally Induced Mechanical Stresses of Egg Cells"Proceedings of the JSME 13-th Bioengineering Conference. No.OO-35. 286-287 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Kosawada, H.Satoh, T.Yamashita: "Piezo Micro Probe Sensor System to Detect Environmental Stresses Induced in Living Cells"Proceedings of Joint Symposium on Bio-Sensing and Bio-Imaging, Yamagata. 24 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Kosawada, S.Yamamura, K.Konno, T.Kanazawa: "Sensing System for Dynamic Properties of the Three Dimensional Minute Living Body"Proceedings of the JSME Annual Meeting Conference 2002. Vol.1, No.02-1. 109-110 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Kosawada, K.Konno, S.Yamamura, T.Kanazawa: "Sensing System to Detect Mechanical Properties of Three Dimensional Minute Living Cells"Proceedings of the JSME 37-th Tohoku Annual Meeting Conference. No.031-1. 180-181 (2003)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2004-04-14  

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