• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2002 Fiscal Year Final Research Report Summary

Advanced multi freedom degree Micromanipulator

Research Project

Project/Area Number 12450100
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Intelligent mechanics/Mechanical systems
Research InstitutionThe University of Tokyo

Principal Investigator

KUDOH Ken-ichi  Graduate School of Engineering, Research Associate, 大学院・工学系研究科, 助手 (90250232)

Co-Investigator(Kenkyū-buntansha) YAMAMOTO Akio  Graduate School of Engineering, Lecturer, 大学院・工学系研究科, 講師 (40313035)
TORII Toru  Graduate School of Engineering, Assistant Professor, 大学院・工学系研究科, 助教授 (60172227)
Project Period (FY) 2000 – 2002
KeywordsMicromanipulator / Microinjection / Micro actuator / Piezoelectric impact drive / Nuclear Transplant / Piezoelectric actuator
Research Abstract

We research is a study about development of a micro manipulator that we use for artificially manipulating plant cell or animal cell. Our aim in this research is to develop a new micro manipulator system which can handle and manipulate one single cell precisely, so for every single cell, we can do injection work accurately and swiftly from every position and angle in order to transplant DNA or nucleus into the cell.
Below are our achievements during the research period
(1) Micro Pipette Drive Device for Nuclear Transplantation.
When we insert micro pipette for nuclear transplantation, the elasticity of the cell become deterrent of the process, and it reduced the efficiency of the work. So. we developed a prototype of super impact micro pipette drive mechanism that is a combination of piezo impact drive mechanism and electromagnetic impact drive mechanism. In the experiment using mouse egg cell, we succeeded to insert the pipette into the cell smoothly without giving damage to the cytoplasm … More . And with the impact power generated by electromagnetic impact drive mechanism, we succeeded to insert the pipette smoothly into other egg cell that is known to have hard cell membrane (for example : pig egg cell) without edge treatment to the tip of the pipette.
(2) Animal Egg Cell Handling Mechanism.
In the process of denucleation of the egg cell, when the nucleus position is close to the holding pipette, it is necessary to amend the position of the nucleus. This work is very complicated and required high skill of the operator. Therefore, we developed cell rotation mechanism using electrostatic. The egg cell rotates at the speed of 30 deg/s with applied voltage of 4V 500 Hz. we also developed a prototype of mechanism using multiple electrode board and put them into layer. This prototype enables three dimensional cell positioning.
(3) Cell Sorter Using Liquid Flow.
We developed a prototype with XY-2axis device using voice coil motors. As the result of having done various kinds of experiments with vibration frequency, vibration amplitude, and liquid quantities as parameters, we succeeded to gather intentionally scattered cell using liquid flow.
(4) We lent the system we developed to below research institutions, and did experiments for performance evaluation.
Kagoshima University, Obihiro University of Agriculture and Veterinary Medicine, Tokyo University of Agriculture, Hirosaki University, St. Mother Hospital. Less

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Michel Budiman, 工藤謙一, 樋口俊郎: "圧電素子と電磁力を組み合わせた駆動機構を用いたマイクロマニピュレータの開発"第13回「電磁力関連のダイナミクス」シンポジウム. 767-770 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] TSUKADA, KUDOH, YAMAMOTO, HIGUCHI, KOBAYASHI, SATO, OOISHI, IIDA: "Development of Oocyte Rotation System for Biological Cell Manipulation"Proceedings of the 32nd ISR (International Symposium on Robotics). 682-685 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] TSUKADA, KUDOH, BUDIMAN, YAMAMOTO, HIGUCHI, KOBAYASHI, SATO, OOISHI, IIDA: "Development of Automated Nuclear Transplantation System"Journal of Mammalian Ova Research. Vol.18. No.3. 106-109 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Michel Budiman, 大石勝昭, 塚田修大, 工藤謙一, 樋口俊郎: "圧電素子を用いた細胞操作用マイクロインジェクションの開発"第14回「電磁力関連のダイナミクス」シンポジウム. 561-564 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] TSUKADA, KUDOH, BUDIMAN, YAMAMOTO, HIGUCHI, KOBAYASHI, SATO: "Rotational Position Control Biological Cell by Electrostatic Force"SICE Annual Conference 2002. 1265-1269 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 工藤 謙一, ブディマン ミシェル, 塚田 修大, 樋口 俊郎, 吉田光敏: "電磁衝撃力を用いた細胞操作用マイクロインジェクションシステムの開発"第15回「電磁力関連のダイナミクス」シンポジウム. (印刷中). (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Michel Budiman, Ken-ichi KUDOH, Toshiro HIGUCHI: "Development of Micro Manipulator Using Combination of Priezoelectric and Electromagnetic Actuator"The 13th symposium on Electromagnetics and Dynamics. 767-700 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] TSUKADA, KUDOH, YAMAMOTO, BUDIMAN, HIGUCHI, KOBAYASHI, SATO, OOISHI, IIDA: "Development of Oocyte Rotation System for Biological Cell Manipulation"Rroceeding of the 32th ISR(International Symposium on Robotics). 682-685 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] TSUKUDA, KUDOH, BUDIMAN, YAMAMOTO, HIGUCHI, KOBAYASHI, SATO, OOISHI, IIDA: "Development of Automated Nuclear Transplantation System"Journal of Mammalian Ova Research. 18 No.3. 106-109 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Budiman, OOISHI, TSUKUDA, KUDOH, HIGUCHI: "Development of Micro Injection for Cell Manipulation Using Piezoelectric"The 14th symposium on Electromagnetics and Dynamics. 561-564 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] TSUKADA, KUDOH, BUDIMAN, YAMAMOTO, HIGUCHI, KOBAYASHI, SATO: "Rotational Position Control Biological Cell by Electrostatic Force"SICE Annual Conference 2002. 1265-1269 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] KUDOH, BUDIMAN, TSUKADA, HIGUCHI, YOSHIDA: "Development of Micro Injection System for Cell Manipulation Using Piezoelectric Impact"The 15th symposium on Electromagnetics and Dynamics. printing. (2001)

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 2004-04-14  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi