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2002 Fiscal Year Final Research Report Summary

Development of model system to analyze the antigen epitope to induce cell-mediated immunity in fish

Research Project

Project/Area Number 12460088
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field General fisheries
Research InstitutionNihon University

Principal Investigator

NAKANISHI Teruyuki  Biological Sciences, veterinary Medicine, Professor, 生物資源科学部, 教授 (00322496)

Co-Investigator(Kenkyū-buntansha) MORITOMO Tadaaki  Biological Sciences, veterinary Medicine, Associate Professor, 生物資源科学部, 助教授 (20239677)
OKAMOTO Nobuaki  Tokyo University of Fisheries, Professor, 水産学部, 教授 (40114912)
Project Period (FY) 2000 – 2002
KeywordsCell-mediated immunity / Cytotoxicity test / MHC class I / IHN virus / DNA vaccination / Rainbow trout / Ginbuna crucian carp / Mixed leukocyte culture
Research Abstract

Fish possess specific antigen recognition molecules as well as B and T lymphocytes and can evoke specific immune responses against a variety of antigens. In fish, however, information on cell-mediated immunity is limited when compared to those on humoral immunity due to the lack of in vivo and in vitro model systems. Vaccine against viral disease is limited in fish despite that viral diseases are serious problem in aquaculture. Development of such vaccines requires identification and characterization of viral antigens which induce protective immunity. It is well known that cell-mediated killing is most important defense mechanisms in the control of viral disease. The objective of this research project is to develop experimental model systems to analyze the protective antigens or epitopes involved in cell-mediated immunity for the development of fish vaccines.
In collaboration with Dr. Okamoto at Tokyo University of Fisheries and Dr. Ototake at National Research Institute of Aquaculture … More we have established several assay systems for specific cell-mediated immunity, particularly on cytotoxic T-cell function employing clonal ginbuna and a syngeneic cell line. Those include the graft-versus host reaction (GVHR) and cell-mediated cytotoxicity (CMC) against allogeneic erythrocytes or cell lines. In the present research project we further pursued the studies and have established model systems for CMC against virus-infected cells and analyzed the protection mechanism using isogeneic ginbuna and cell lines together with fish viruses. We also extensively analyzed the MHC polymorphism in rainbow trout and found that trout cell line (RTG-2) shares the same MHC class I allele with homozygous clonal rainbow trout (C25 clone). We demonstrated MHC-restricted cell-mediated cytotoxicity against virus-infected cell lines using lymphocytes from C25 clonal trout sensitized by DNA immunization with IHNV-G or by infection with IHNV and RTG-2 cells as target. Experimental model systems established in the present study can be applied to investigate the protective antigens or epitopes involved in cell-mediated immunity for the development of fish vaccines as well as to investigate the recognition and killing g mechanism of specific cell-mediated immunity. Less

  • Research Products

    (22 results)

All Other

All Publications (22 results)

  • [Publications] Xia, C., Kiryu, I., J.M.Dijkstra, T.Azuma, T.Nakanishi, M.Ototake: "Differences in MHC class I between strains of rainbow trout (Oncorhynchus mykiss)"Fish & Shellfish Immunol.. 12. 287-301 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Okubo, K., Qin Qiwei, K.Aoyagi, K.Yamano, M.Ototake, T.Nakanishi, H.Fukuda, J.M.Dijkstra: "Ubiquitin genes in rainbow trout (Oncorhynchus mykiss)"Fish & Shellfish Immunol.. 12. 335-351 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Somamoto, T., T.Nakanishi, N Okamoto: "Roles of specific cell-mediated cytotoxicity in protecting fish from viral infections"Virology. 297. 120-127 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 乙竹充, J.M.Dijkstra, 桐生郁也, 吉浦康寿, 藤原篤志, U.Fischer, 中西照幸: "ニジマスMHC遺伝子の多様性及び機能の解析"水産育種. 32. 67-74 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Dijkstra, J.M., B.Kollner, K.Aoyagi, I.Denda, A.Kuroda, M.Ototake, K.Hashimoto, T.Nakanishi, Fischer, U.: "The rainbow trout classical MHC class I molecule Onmy-UBA ^*501 is expressed in similar cell types as mammalian classical MHC class I molecules"Fish & Shellfish Immunol.. 14. 1-23 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] J.M.Dijkstra, Y.Yoshiura, I.Kiryu, K.Aoyagi, B.Kollner, U.Fischer, T.Nakanishi, M.Ototake: "The promoter of the classical MHC class I locus in rainbow trout (Oncorhynchus mykiss)"Fish & Shellfish Immunol.. 14. 177-185 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nakanishi, T., U.Fischer, J.M.Dijkstra, M.Ototake: "MHC-restricted cell-mediated cytotoxicity in fish"Fisheries Science Vol.68, Suppl.. (in press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Somamoto, T., T.Nakanishi, N.Okamoto: "Specific cytotoxic cells of ginbuna crucian carp are responsible for in vivo clearance of carp haematopoietic necrosis virus(CHNV)"Fisheries Science Vol.68, Suppl.. (in press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 中西照幸, J.M.Dijkstra, 桐生郁也, 乙竹充: "魚類の免疫系(免疫系の遺伝子、8.主要組織適合遺伝子複合体(MHC)"恒星社厚生閣. 163(114-127) (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Somamoto, T., Nakanishi, T. and Okamoto, N.: "Specific cell-mediated cytotoxicity against a virus-infected syngeneic cell line in isogeneic ginbuna crucian carp"Dev. Comp. Immunol.. 24. 633-640 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Dijkstra, J.M., U. Fischer, I. Denda, M. Ototake and T. Nakanishi: "Exogenous antigens and the stimulation of MHC class I restricted cell-mediated cytotoxicity: possible strategies for fish vaccines"Fish & Shellfish Immunol.. 11. 437-458 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nakanishi, T., U. Fischer, J.M. Dijkstra, S. Hasegawa, T. Somamoto, N. Okamoto and M. Ototake: "Cytotoxic T cell function in fish"Dev. Comp. Immunol.. 26. 131-139 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Aoyagi K., J.M. Dijkstra, C. Xia, I. Denda, M. Ototake, K Hashimoto, and T. Nakanishi: "Classical MHC class I genes composed of highly divergent sequence lineages share a single locus in rainbow trout (Oncorhynchus mykiss)"J. Immunology. 168. 260-273 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Xia, C., Kiryu, I., J.M. Dijkstra, T. Azuma, T. Nakanishi and M. Ototake: "Differences in MHC class I between strains of rainbow trout (Oncorhynchus mykiss)"Fish & Shellfish Immunol.. 12. 287-301 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Okubo, K., Qin Qiwei, K. Aoyagi, K. Yamano, M. Ototake, T. Nakanishi, H. Fukuda and J. M. Dijkstra: "Ubiquitin genes in rainbow trout (Oncorhynchus mykiss)"Fish & Shellfish Immunol.. 12. 335-351 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Q. W. Qin, M. Ototake, H. Nagoya and T. Nakanishi: "Graft-versus-host reaction (GVHR) in clonal amago salmon, Oncorhunchus rhodurus"Vet. Immunol. Immunopathol.. 89. 83-89 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Somamoto, T., T. Nakanishi, and N Okamoto: "Roles of specific cell-mediated cytotoxicity in protecting fish from viral infections"Virology. 297. 120-127 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Dijkstra, J.M., B. Kollner, K. Aoyagi, I. Denda, A. Kuroda, M. Ototake, K. Hashimoto: "The rainbow trout classical MHC class I molecule Onmy-UBA*501 is expressed in similar cell types as mammalian classical MHC class I molecules"Fish & Shellfish Immunol.. 14. 1-23 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ototake, M., J.M. Dijkstra, I. Kiryu, Y. Yoshiura, A. Fujiwara, U. Fischer and T. Nakanishi: "Structure and functions of MHC class I genes in rainbow trout Oncorhynchus mykiss"Fish Genetics and Breeding Science. 32. 67-74 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Dijkstra, J. M., Y. Yoshiura, I. Kiryu, K. Aoyagi, B. Kollner, U. Fischer, T. Nakanishi and M. Ototake: "The promoter of the classical MHC class I locus in rainbow trout (Oncorhynchus mykiss)"Fish & Shellfish Immunol.. 14. 177-185 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nakanishi, T., U. Fischer, J.M. Dijkstra and M. Ototake: "MCH-restricted cell-mediated cytotoxicity in fish"Fisheries Science. Vol. 68, Supple. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Somamoto, T., T. Nakanishi and N. Okamoto: "Specific cytotoxic cells of ginbuna crucian carp are responsible for in vivo clearance of carp haematopoietic necrosis virus (CHNV)"Fisheries Science. Vol. 68, Supple. (in press).

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2004-04-14  

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