2001 Fiscal Year Final Research Report Summary
Functional recovery of the cerebral infarction following transplantation of the genetically-engineering human neural stem cells
| Project/Area Number |
12470295
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | Sapporo Medical University |
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Principal Investigator |
HONMOU Osamu Sapporo Medical University School of medicine, Department of Neurosurgery, Assistant Professor, 医学部, 講師 (90285007)
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| Co-Investigator(Kenkyū-buntansha) |
UEDE Teiji Sapporo Medical University School of Medicine, Department of Neurosurgery, Associate Professor, 医学部, 助教授 (90160184)
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| Project Period (FY) |
2000 – 2001
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| Keywords | adult / gene / human / infarction / neural stem cell / stroke / transplantation |
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| Research Abstract |
Introduction: Although it has generally been assumed that the adult brain is incapable of significant self-repair because of a lack of neurogenesis in the adult mammalian central nervous system (CNS), several studies have reported that the adult mammalian brain harbors neural stem cells that retain the potential for both neural production and differentiation in experimental animal models. These findings offer the prospect of the presence of neural precursors in the adult human brain. The objective of this study was to identify neural stem cells in the adult human CNS and study whether they repair the ischemic brain.
Methdos: Clonal neural stem cells derived from adult human brain were established in vitro. They were genetically marked with LacZ gene and was transplanted into the ischemic brain of the experimental animal. It was investigated if neural tissue reconstruction occurred following transplantation.
Results: Single-cell clonal analysis demonstrated self-renewing and multipotential properties of neural stem cells derived from the adult human brain in vitro. Histological examination of the ischemic lesion following transplantation revealed that the transplanted human cells reconstructed the neural tissue. The transplanted stem cell in the contra-lateral hemisphere also migrated into the ischemic lesion of the another hemisphere, and repaired the damaged brain following ischemic stress. The electrophysiological and behavior studies also proved the functional recovery of the ischemic model animals following the human neural stem cell transplantation.
Conclusion: Adult human brain harbors neural stem cells that retain the potential for both neural production and differentiation. Transplantation of clonal neural stem cells derived from adult human brain repaired the ischemic neural tissue in the mammalian adult CNS.
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[Publications] Honmou, O., Sasaki, M., Oka, S., Takahashi, A., Tanooka, A., Uede, T., Hashi, K.: "A cell therapy for the CNS diseases"Restorative Neurology and Neuroscience. (in press). (2002)
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「研究成果報告書概要(欧文)」より
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[Publications] Takahashi, A., Honmou, O., Sasaki, M., Oka, S., Uede, T., Hashi, K.: "Transplantation of human neural stem cells into the ischemic lesion of the rat brain"Restorative Neurology and Neuroscience.. (in press). (2002)
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「研究成果報告書概要(欧文)」より
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[Publications] Sasaki, M., Honmou, O., Akiyama, Y., Uede, U., Hashi, K., and Kocsis, J. D: "Transplantation of an acutely isolated bone marrow fraction repairs demyelinated adult rat spinal cord axons"Glia.. 35. 26-34 (2001)
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「研究成果報告書概要(欧文)」より
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[Publications] Akiyama, Y., Honmou, O., Kato, T., Uede, T., Hashi, K. and Kocsis, J. D: "Transplantation of Clonal Neural Precursor Cells Derived from Adult Human Brain Establishes Functional Peripheral Myelin in the Rat spinal Cord"Experimental Neurology.. 167. 27-39 (2001)
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「研究成果報告書概要(欧文)」より
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