2001 Fiscal Year Annual Research Report

歯胚と歯原性腫瘍の分子病理学的研究

Research Project

Project/Area Number	12470385
Research Institution	Okayama University
Principal Investigator	永井教之岡山大学, 大学院・医歯学総合研究科, 教授 (90085770)
Co-Investigator(Kenkyū-buntansha)	GUNDUZ Mehmet 岡山大学, 大学院・医歯学総合研究科, 助手 (70333507) 辻極秀次岡山大学, 大学院・医歯学総合研究科, 助手 (70335628) 長塚仁岡山大学, 大学院・医歯学総合研究科, 助教授 (70237535) 小守壽文岡山大学, 大学院・医学系研究科, 助手 (00252677) 長岡紀幸岡山大学, 歯学部, 教職員 (70304326)
Keywords	歯胚 / BMP / 基底膜 / IV型コラーゲン / 成長因子 / Cbfal / 歯原性腫瘍
Research Abstract	器官形成の上皮間葉相互作用に関わる因子として注目されているBMPファミリーが,歯胚分化過程のいかなる時期で発現しているかをin situ hybridization法を用いて検索した。胎生11日にすでに発現が見られたのはBMP-4および7であり,胎生12日になるとBMP-2の発現も見られた。一方,歯胚基底膜の主要構成成分であるIV型コラーゲンのα1からα6までの6種類のα鎖の歯牙発生過程での局在と消長を免疫組織学的に観察した結果、歯胚上皮基底膜にα鎖の部位および時期特異的な局在と組み合わせが存在することを明らかになった。特に帽状期後期からの内エナメル上皮基底膜には、α1,α2,α4鎖を含む他の臓器にはない特異なα鎖の組み合わせが存在し、その組み合わせが歯胚特異的な形態形成、細胞分化に関与していることが示唆された。歯原性腫瘍におけるIV型コラーゲンは,エナメル上皮腫ではα1,2,5,6鎖から構成されていたが,腺様歯原性腫瘍,エナメル上皮線維腫ではα4鎖の特異的な局在が見られた。歯原性腫瘍においても歯胚と同様に特異なα鎖の局在が腫瘍細胞の分化に関与していることが考えられた。 Cbfa1ノックアウトマウスにおける臼歯の歯胚発育は、組織学的には帽状期で停止しており,IV型コラーゲンα鎖の分布もα5鎖が特異な染色性を示すなどの所見が認められた。一方,切歯では基質蛋白の遺伝子発現の面からは象牙芽細胞およびエナメル芽細胞にまで分化しており,臼歯とは異なるシグナル伝達系が存在する可能性が示唆された。

Research Products

(15 results)

All Other

All Publications (15 results)

[Publications] Noriyuki NAGAI: "Localization of type IV collagen α-1 to α-6 chains in basement membrane during mouse molar germ development"the International Journal of Developmental Biology. 45・7. 827-831 (2001)
[Publications] Hitoshi NAGATSUKA: "Quantitation of epidermal growth factor gene amplification by competitive polymerase reaction in premalignant and malignant oral epithelial lesions"Oral Oncology. 30・6. 599-604 (2001)
[Publications] Eiki YAMACHIKA: "Characteristics of Bone Morphogenetic Protein-induced Chondroid Bone : Histochemical, Immunohistochemical and in situ Hybridization Examinationscleidocranial dysplasia and cleft lip"Journal of Oral Pathology and medicine. 30・6. 381-383 (2001)
[Publications] Hitoshi NAGATSUKA: "Site dependence of Bone Formation Induced by rhBMP-2 : Comparing Rat Parietal Bone and Heterotopic Back Region"Engineering Materials. 192-195. 511-514 (2001)
[Publications] Akira YAMASAKI: "Expression of Type I, Type II, and Type X Collagen Genes during Altered Endochondral Ossification in the Femoral Epiphysis of Osteosclerotic (oc/oc) Mice"Calcified Tissue International. 68. 53-60 (2001)
[Publications] Yoshiya UEYAMA: "Initial tissue response to anti-washout apatite cement in the rat palatal region : comparison with conventional apatite"Journal of Biomedical Materials Research. 55・4. 652-660 (2001)
[Publications] N.MIZUKAWA: "Human alpha-and bera-defensin immunoreactivity in oral mucoepidemoid carcinomas"Anticancer Research. 21. 2171-2174 (2001)
[Publications] 辻極秀次: "rhBMP-2/コラーゲン固定化によるST2細胞への影響とシグナル伝達系遺伝子の発現"岡山歯学学会雑誌. 20・1. 42-55 (2001)
[Publications] K.NISHIDA: "Involvement of nitric oxide in chondrocyte cell death in chondro-osteophyte formation"Osteoarthritis and Cartilage. 9・3. 232-237 (2001)
[Publications] O.MITSUKUNI: "Pronostic Significance of p27 and Ki-67 Expression in Mucoepidermoid Carcinima of the Intraoral Minor Salivary Gland"Modern Pathology. 14・10. 1008-1014 (2001)
[Publications] Masaru MURATA: "Combination of Recombinant Human BMP-2 and Atelocollagen Implant with Cortical Perforations Bone Augmentation"Journal of Hard Tissue Biology. 10・1. 1-6 (2001)
[Publications] Y.J.LEE: "Phenomelanin and Eumelanin of Malignant Melanomas and Melanosis in Oral Mucosa"Journal of Hard Tissue Biology. 10・2. 89-95 (2001)
[Publications] K.SUGATA: "Genetic Alteration of Penicllin Non-Susceptible Streptococcus pneumoniae Observed throughout Recurrence of Acute Otitis Media Detected by Amplified Fragment Length Polymorphism Analysis"Acta Medica Okayama. 55・3. 167-174 (2001)
[Publications] Y.YASUOKA: "Combination of tumor necrosis factor-alpha and interferon-alpha induces apoptotic cell death through a c-myc dependent pathway in p53 mutant H226br non-small cell lung cancer cell line"Experimental Cell Research. 271. 214-222 (2001)
[Publications] Toshiyuki KAWAKAMI: "Characteristics of Bone Morphogenetic Protein-induced Chondroid Bone : Histochemical, Immunohistochemical and in situ Hybridization Examinations"The Journal of International Medical Research. 29・6. 480-487 (2001)

2001 Fiscal Year Annual Research Report

歯胚と歯原性腫瘍の分子病理学的研究

Principal Investigator

永井 教之 岡山大学, 大学院・医歯学総合研究科, 教授 (90085770)

Research Products

[Publications] Noriyuki NAGAI: "Localization of type IV collagen α-1 to α-6 chains in basement membrane during mouse molar germ development"the International Journal of Developmental Biology. 45・7. 827-831 (2001)

[Publications] Hitoshi NAGATSUKA: "Quantitation of epidermal growth factor gene amplification by competitive polymerase reaction in premalignant and malignant oral epithelial lesions"Oral Oncology. 30・6. 599-604 (2001)

[Publications] Eiki YAMACHIKA: "Characteristics of Bone Morphogenetic Protein-induced Chondroid Bone : Histochemical, Immunohistochemical and in situ Hybridization Examinationscleidocranial dysplasia and cleft lip"Journal of Oral Pathology and medicine. 30・6. 381-383 (2001)

[Publications] Hitoshi NAGATSUKA: "Site dependence of Bone Formation Induced by rhBMP-2 : Comparing Rat Parietal Bone and Heterotopic Back Region"Engineering Materials. 192-195. 511-514 (2001)

[Publications] Akira YAMASAKI: "Expression of Type I, Type II, and Type X Collagen Genes during Altered Endochondral Ossification in the Femoral Epiphysis of Osteosclerotic (oc/oc) Mice"Calcified Tissue International. 68. 53-60 (2001)

[Publications] Yoshiya UEYAMA: "Initial tissue response to anti-washout apatite cement in the rat palatal region : comparison with conventional apatite"Journal of Biomedical Materials Research. 55・4. 652-660 (2001)

[Publications] N.MIZUKAWA: "Human alpha-and bera-defensin immunoreactivity in oral mucoepidemoid carcinomas"Anticancer Research. 21. 2171-2174 (2001)

[Publications] 辻極秀次: "rhBMP-2/コラーゲン固定化によるST2細胞への影響とシグナル伝達系遺伝子の発現"岡山歯学学会雑誌. 20・1. 42-55 (2001)

[Publications] K.NISHIDA: "Involvement of nitric oxide in chondrocyte cell death in chondro-osteophyte formation"Osteoarthritis and Cartilage. 9・3. 232-237 (2001)

[Publications] O.MITSUKUNI: "Pronostic Significance of p27 and Ki-67 Expression in Mucoepidermoid Carcinima of the Intraoral Minor Salivary Gland"Modern Pathology. 14・10. 1008-1014 (2001)

[Publications] Masaru MURATA: "Combination of Recombinant Human BMP-2 and Atelocollagen Implant with Cortical Perforations Bone Augmentation"Journal of Hard Tissue Biology. 10・1. 1-6 (2001)

[Publications] Y.J.LEE: "Phenomelanin and Eumelanin of Malignant Melanomas and Melanosis in Oral Mucosa"Journal of Hard Tissue Biology. 10・2. 89-95 (2001)

[Publications] K.SUGATA: "Genetic Alteration of Penicllin Non-Susceptible Streptococcus pneumoniae Observed throughout Recurrence of Acute Otitis Media Detected by Amplified Fragment Length Polymorphism Analysis"Acta Medica Okayama. 55・3. 167-174 (2001)

[Publications] Y.YASUOKA: "Combination of tumor necrosis factor-alpha and interferon-alpha induces apoptotic cell death through a c-myc dependent pathway in p53 mutant H226br non-small cell lung cancer cell line"Experimental Cell Research. 271. 214-222 (2001)

[Publications] Toshiyuki KAWAKAMI: "Characteristics of Bone Morphogenetic Protein-induced Chondroid Bone : Histochemical, Immunohistochemical and in situ Hybridization Examinations"The Journal of International Medical Research. 29・6. 480-487 (2001)

永井教之岡山大学, 大学院・医歯学総合研究科, 教授 (90085770)