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2002 Fiscal Year Final Research Report Summary

Liquid ventilation as a bridging to lung transplantation

Research Project

Project/Area Number 12557110
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section展開研究
Research Field Thoracic surgery
Research InstitutionTohoku University

Principal Investigator

KONDO Takashi  Institute of Development, Aging and Cancer, Tohoku University, Department of Thoracic Surgery, Professor, 加齢医学研究所, 教授 (10195901)

Co-Investigator(Kenkyū-buntansha) ENDO Chiaki  Tohoku University School of Medicine, Department of Thoracic Surgery, Assistant Professor, 医学部附属病院, 助手 (80333813)
OKADA Yoshinori  Institute of Development, Aging and Cancer, Tohoku University, Department of Thoracic Surgery, Assistant Professor, 加齢医学研究所, 助手 (90323104)
MATSUMURA Yuji  Tohoku University School of Medicine, Department of Thoracic Surgery, Assistant Professor, 医学部附属病院, 助手 (80281997)
HOSHIKAWA Yasushi  Tohoku University School of Medicine, Department of Thoracic Surgery, Assistant Professor, 医学部附属病院, 助手 (90333814)
Project Period (FY) 2000 – 2002
KeywordsLiquid ventilation / FC-77 / rat / human pulmonary endothelial cella / human alveolar epithelial cell / respiratory failure / oxygenation
Research Abstract

There has been no applicable ventilation devises to support patients with chronic with respiratory failure. In the present research project, we sought to evaluate whether liquid ventilation using across gut and/or peritoneum would be function to increase oxygenation. We utilized FC-77 in not only cell experiments, but also anesthetized rat models. In cell experiments, we mixed oxygenated FC-77 with human peripheral venous blood in a test tube. We found a marked increase in Pao2 and a decrease in PaCO2 in the first minute following mixture. We also found that FC-77 was able to deliver O2 and absorb CO2 across the monolayers of human pulmonary arterial endothelial cells and human alveolar epithelial cells (A549) in the transwell insert systems. In rat experiments, rats were anesthetized and ventilated with low tidal volume (VT=5ml/kg) at low ventilation rate (f=20), and then oxygenated FC-77 (20ml/kg) was introduced into colon or peritoneal cavity. We found a progressive increase in PaO2 in both colon and or peritoneum experiments over 4 hours following administration of FC compound. However, peritoneal administration seemed to be somewhat superior not only in oxygenation, but also in the ability of removing C02. We believe that FC-77 would be applicable in the rescue of patients with chronic with respiratory failure. Our data obtained in this project indicate that liquid ventilation across the peritoneum would potentially become possible in near future.

  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] Okada Y, et al.: "Transient cold presevation alone stimulates tumor necrosis factor-alpha gene expression in a model of rat sngeneic rat transplantation"Translplantation Proc.. 34. 1111-1113 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Matsumura Y, Okada Y, et al.: "Assessment of brain-dead cadaver donor for lung transplantation"Translplantation Proc.. 34. 2585-2586 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Noda, M, Kondo T: "Single dexamethasone injection increases alveolar fluid clearance in adult rats"Critical Care Med. 31(In press). (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tsubochi H, Kondo T: "Early changes in alveolar fluid clearance by nitric oxide after endotoxin instillation in rats"Am J Respir Crit Care Med. 167. 205-210 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ishida I, Kondo T: "Hypoxia diminishes Toll-like receptor 4 expression through reactive oxygen species generated by mitochondria in endothelial cells"J Immunol. 169. 2069-2075 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Okada Y et al.: "Transient cold preservation alone stimulates tumor necrosis factor-alpha gene expression in a cold rat synergic rat transplantation"Transplant Proc. 34. 1111-1113 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Matsumura Y et al.: "Assessment of brain-dead cadaver donor for lung transplantation"Transplant Proc. 34. 2585-2586 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Noda M, et al.: "Single dexamethasone injection increases alveolar fluid clearance in adult rats"Crit Care Med. In press. (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tsubochi H, et al.: "Early Changes in Alveolar Fluid Clearance by Nitric Oxide Following Endotoxin Instillation in Rats"Am J Respir Crit Care Med. 167. 205-210 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ishida I, et al.: "Hypoxia diminishes Toll-like receptor 4 expression through reactive oxygen species generated by mitochondria in endothelial cells"J Immunol. 169. 2069-2075 (2002)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2004-04-14  

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