2001 Fiscal Year Final Research Report Summary
Syriltes and Biologlacal Enaluativns of Fulkren-Glycosidet.
| Project/Area Number |
12660096
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Bioproduction chemistry/Bioorganic chemistry
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| Research Institution | Nagoya University |
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Principal Investigator |
NISHIDA Yoshihiro Nagoya University Engineering, Ass. Prof., 工学研究科, 助教授 (80183896)
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| Co-Investigator(Kenkyū-buntansha) |
MATSUDA Kazuhiro Nagoya University National Cancer Inst. Chief, ウイルス部, 主任研究官 (80251502)
KOBAYASHI Kazukiyo Nagoya University Engineering, Ass. Prof., 工学研究科, 教授 (10023483)
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| Project Period (FY) |
2000 – 2001
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| Keywords | Glycosides / Fullerene / Glycoconjugate / Glycosylation / Glycosyltromsferase / Shiga toxins / Active Oxygen / Fullerenal |
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| Research Abstract |
In this research project, a new type of glycoconjugates, namely, "fullerene g ycoconjugates ' was designed and developed. The C60 conjugates are composed of biologically active active giycosides introduced onto the surface via an N-glycoside linkage. The different property of the two molecules regarding from both physicchemical and biological aspects is expected to induce a novel class of biologically active compounds.
Prior to the synthesis of the'Tullerene glycoconjugates", an efficient glycosylation method was deveioped, which involved the use of new thio-glycosyl donors and galactosyltransferase. The new thio-glycosyl donor was applied conveniently to the formation of globotn- and biosides possessing high biological potential to trap Shiga toxins and deactivate the toxins. A bovine galactosyltransferase was found to catalyse a novel class of enzymic reactions applicable to non-natural L-sugars and acyclic substrates.
Several types of biologiclaly active saccharides were coupled with C60 fullerene via thermal couplmg between a glycoylazide and C60. The derived fullerene glycococonjugates as well as fullerenol conjugates were water-miscible and proved to show high biological potential to block carbohydrate-protein binding involved in many biological phenomena.
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[Publications] H. Kato, K. Suenaga, M Mikawa, M. Okumura, N. Miwa, A. Yashiro, H. Fujimura, A. Mizuno, Y.Nishida, K. Kobayashi, H. Shinohara: "Syntheses and EELS Characterization of Water-Soluble Multi-Hydroxyl Gd@C82 Fullerenols"Chem. Phys. Lett.. 324. 255-259 (2000)
Description
「研究成果報告書概要(欧文)」より
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