2002 Fiscal Year Final Research Report Summary
Anchorage independent cell cycle start
| Project/Area Number |
12670107
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General medical chemistry
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| Research Institution | The University of Tokyo, |
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Principal Investigator |
JINNO Shigeki The University of Tokyo, Graduate School of Medicine, Research Associate, 大学院・医学系研究科, 助手 (10251224)
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| Co-Investigator(Kenkyū-buntansha) |
NAGATA Akihisa The University of Tokyo, Graduate School of Medicine, Lecturer, 大学院・医学系研究科, 講師 (50155933)
OKAYAMA Hiroto The University of Tokyo, Graduate School of Medicine, Professor, 大学院・医学系研究科, 教授 (40111950)
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| Project Period (FY) |
2000 – 2002
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| Keywords | Cell cycle / Anchorage / NRK / E2F / Cdk / Cdc6 |
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| Research Abstract |
Fibroblasts need anchorage to extracellular matrix to transit from G1 to S phase, but no longer after oncogenic transformation. We report that Cdc6 protein essential for the activation of replication origins requires anchorage or oncogenic stimulation for its execution. Upon anchorage loss, Cdc6 expression is shut of both transcriptionally and post-transcriptionally in a rat fibroblast despite enforced activation of E2F-dependent promoters. However, stimulation of this cell with oncogenic growth factors suppresses this shutoff and concurrently activates Cdk2 and Cdk6/4, thereby overriding the anchorage requirement for the G1-S transition and consequently enabling cells to perform anchorage-independent S phase entry. Analysis with enforced expression of Cdc6 indicates that the G1 cyclin-dependent kinases and Cdc6 constitute major cell cycle targets for the restriction of the G1-S transition by anchorage loss.
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[Publications] Hiroi, N., Ito, T., Yamamoto, H., Ochiya, T., Jinno, S., Okayama: "Mammalian Rcd1 is a novel transcriptional cofactor that mediates retinoic acid-induced cell differentiation"The EMBO Journal. 21. 5235-5244 (2002)
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「研究成果報告書概要(和文)」より
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[Publications] Nakamura, E., Isobe, H., Tomita, N., Sawamura, M.,Jinno, S., and Okayama, H.: "Functionalized Fullerene as an Artificial Vector for Transfection"Angew. Chem. Int. Ed.. 39-23. 4254-4257 (2000)
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「研究成果報告書概要(欧文)」より
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[Publications] Hiroi, N., Ito, T., Yamamoto. H., Ochiya, T., Jinno, S., and Okayama, H.: "Mammalian Rcd1 is a novel transcriptional cofactor that mediates retinoic acid-induced cell differentiation"The EMBO Journal. 21. 5235-5244 (2002)
Description
「研究成果報告書概要(欧文)」より
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