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2002 Fiscal Year Final Research Report Summary

Anchorage independent cell cycle start

Research Project

Project/Area Number 12670107
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General medical chemistry
Research InstitutionThe University of Tokyo,

Principal Investigator

JINNO Shigeki  The University of Tokyo, Graduate School of Medicine, Research Associate, 大学院・医学系研究科, 助手 (10251224)

Co-Investigator(Kenkyū-buntansha) NAGATA Akihisa  The University of Tokyo, Graduate School of Medicine, Lecturer, 大学院・医学系研究科, 講師 (50155933)
OKAYAMA Hiroto  The University of Tokyo, Graduate School of Medicine, Professor, 大学院・医学系研究科, 教授 (40111950)
Project Period (FY) 2000 – 2002
KeywordsCell cycle / Anchorage / NRK / E2F / Cdk / Cdc6
Research Abstract

Fibroblasts need anchorage to extracellular matrix to transit from G1 to S phase, but no longer after oncogenic transformation. We report that Cdc6 protein essential for the activation of replication origins requires anchorage or oncogenic stimulation for its execution. Upon anchorage loss, Cdc6 expression is shut of both transcriptionally and post-transcriptionally in a rat fibroblast despite enforced activation of E2F-dependent promoters. However, stimulation of this cell with oncogenic growth factors suppresses this shutoff and concurrently activates Cdk2 and Cdk6/4, thereby overriding the anchorage requirement for the G1-S transition and consequently enabling cells to perform anchorage-independent S phase entry. Analysis with enforced expression of Cdc6 indicates that the G1 cyclin-dependent kinases and Cdc6 constitute major cell cycle targets for the restriction of the G1-S transition by anchorage loss.

  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] Nakamura, E., Isobe, H., Tomita, N., Sawamura, M., Jinno, S.: "Functionalized Fullerene as an Artificial Vector for Transfection"Angew.Chem.Int.Ed.. 23. 4254-4257 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Lin, J., Jinno, S., Okayama, H.: "Cdk6-cyclin D3 complex evades inhibition by inhibitor proteins and uniquely controls cell's proliferation competence"Oncogene. 20. 2000-2009 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Jinno, S., Lin, J., Yageta, M., Okayama, H.: "Oncogenic cell cycle start control"Mutatin Reseach. 477. 23-29 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] sobe H, Tomita N., Jinno S., Okayama H., Nakamura E.: "Synthesis and transfection capability of multi-functionalized fullerene polyamine"Chem.Lett.. 30. 1214-1215 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Jinno, S., Yageta, M., Nagata, A., Okayama, H.: "Cdc6 requires anchorage for its expression"Oncogene. 21. 1777-1784 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hiroi, N., Ito, T., Yamamoto, H., Ochiya, T., Jinno, S., Okayama: "Mammalian Rcd1 is a novel transcriptional cofactor that mediates retinoic acid-induced cell differentiation"The EMBO Journal. 21. 5235-5244 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Chen, Q., Lin, J., Jinno, S., Okayama, H.: "Overexpression of Cdk6-cyclin D3 highly sensitizes cells to physical and chemical transformation"Oncogene. 22. 992-1001 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nakamura, E., Isobe, H., Tomita, N., Sawamura, M.,Jinno, S., and Okayama, H.: "Functionalized Fullerene as an Artificial Vector for Transfection"Angew. Chem. Int. Ed.. 39-23. 4254-4257 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Lin, J., Jinno, S., and Okayama, H.: "Cdk6-cyclin D3 complex evades inhibition by inhibitor proteins and uniquely controls cell's proliferation competence"Oncogene. 20. 2000-2009 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Jinno, S., Lin, J., Yageta, M., and Okayama, H.: "Oncogenic cell cycle start control"Mutatin Reseach. 477. 23-29 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Isobe H, Tomita N., Jinno S., Okayama H. and Nakamura E.: "Synthesis and transfection capability of multi-functionalized fullerene polyamine"Chem. Lett.. 30. 1214-1215 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Jinno, S., Yageta, M., Nagata, A., and Okayama, H.: "Cdc6 requires anchorage for its expression"Oncogene. 21. 1777-1784 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hiroi, N., Ito, T., Yamamoto. H., Ochiya, T., Jinno, S., and Okayama, H.: "Mammalian Rcd1 is a novel transcriptional cofactor that mediates retinoic acid-induced cell differentiation"The EMBO Journal. 21. 5235-5244 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Chen, Q., Lin, J., Jinno, S., and Okayama, H.: "Overexpression of Cdk6-cyclin D3 highly sensitizes cells to physical and chemical transformation"Oncogene. 22. 992-1001 (2003)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2004-04-14  

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