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2001 Fiscal Year Final Research Report Summary

Development of new strategy in surgical oncology introduced with regulation of micometastasis

Research Project

Project/Area Number 12671156
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General surgery
Research InstitutionOsaka University

Principal Investigator

FUJIWARA Yoshiyuki  Osaka University, Graduate School of Medicine, Assistant Professor, 医学系研究科, 助手 (40314330)

Co-Investigator(Kenkyū-buntansha) YONO Masahiko  Osaka University, Graduate School of Medicine, Associate Professor, 医学系研究科, 助教授 (70273646)
NAKAMORI Shoji  Osaka University, Graduate School of Medicine, Lecturer, 医学系研究科, 講師 (70294080)
MONDEN Morito  Osaka University, Graduate School of Medicine, Professor, 医学系研究科, 教授 (00127309)
Project Period (FY) 2000 – 2001
Keywordsgenetic diagnosis / micrometastasis / RT-PCR / gastrointestinal cancer / breast cancer / lung cancer / intra - operative diagnosis
Research Abstract

In this study, we have developed the genetic diagnosis with multiple-marker RT-PCR assay in order to detect micrometastases in solid tumors such as gastric, esophageal, colorectal, lung, pancreas, liver, bile duct and breast carcinomas.
With the use of surgical specimens such as lymph node and blood, we demonstrated the usefulness and significance of this assay in these types of cancer. Furthermore, we have developed the quality of control in this genetic detection, including the sampling procedure without cross-contamination for introducing genetic diagnosis into the clinical use. We also developed intra-operative genetic diagnosis of lymph nodes for the selection of cervical lymphadenectomy in esophageal cancer surgery.
We applied this rapid genetic diagnosis for 72 patients performed with esophageal cancer surgery by now and showed the usefulness and significance of this genetic diagnosis as the operative indication of cervical lymphadenectomy.
We will improve the genetic system (more rapid and more convenient way) to apply it for many kinds of cancer surgery in the future. Furthermore, precise molecular staging will detect the high-risk group for cancer recurrence, which will be subjected to the additional therapy.

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Miyamoto A., Fujiwara Y. et al.: "Development of a multiple-marker RT-PCR assay for detection of micrometastases of hepatocellular carcinoma"Dig Dis Sci. 45. 1376-1382 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Miyake Y., Fujiwara Y., et al.: "Quantification of micrometastases in lymph nodes of colorectal cancer using real-time fluorescence polymerase chain reaction"Int J Oncol. 16. 289-293 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Fujiwara Y. et al.: "Prevention of cross-contamination during sampling procedure in molecular detection for cancer micrometastasis"Cancer Letters. 153. 109-111 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Miyake Y., Fujiwara Y. et al.: "Extensive micrometastases to lymph nodes as a marker for rapid recurrence of colorectal cancer : A study of lymphatic mapping1"Clin Can Res. 7. 1350-1357 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Iwao K., Fujiwara Y. et al.: "Isolation of a novel human lung-specific gene, lunx, a potential molecular marker for detection of micrometastasis in non-small-cell lung cancer"Int J Cancer. 91. 433-437 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Okada Y., Fujiwara Y. et al.: "Genetic detection of lymph node micrometastases in patients with gastric carcinoma by multiple-marker reverse transcriptase-polymerase chain reaction assay"Cancer. 92. 2056-2064 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Miyamoto A., Fujiwara Y. et al.: "Development of a multiple-marker RT-PCR assay for detection of micrometastases of hepatocellular carcinoma"Dig Dis Sci. 45. 1376-1382 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Miyake Y., Fujiwara Y. et al.: "Quantification of micrometastases in lymph nodes of colorectal cancer using real-time fluorescence polymerase chain reaction"Int J Oncol. 16. 289-293 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Fujiwara Y. et al.: "Prevention of cross-contamination during sampling procedure in molecular detection lor cancer micrometastasis"Cancer Letters. 153. 109-111 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Miyake K, FujiwaraY. et al.: "Extensive micrometastases to lymph nodes as a marker for rapid recurrence of colorectal cancer : A study of lymphatic mapping1"Clin Can Res. 7. 1350-1357 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Iwao K., Fujiwara Y. et al.: "Isolation of a novel human lung-specific gene, lunx, a potential molecular marker for detection of micrometastasis in non-small-cell lung cancer"Int J Cancer. 91. 433-437 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Okada Y., Fujiwara Y. et al.: "Genetic detection of lymph node micrometastases in patients with gastric carcinoma by multiple -marker reverse transcriptase-polymerase chain reaction assay"Cancer. 92. 2056-2064 (2001)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2003-09-17  

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