2002 Fiscal Year Final Research Report Summary
Development of immunocontraceptive vaccine using GPI-anchored proteins on the surface of human sperm
| Project/Area Number |
12671624
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Jichi Medical School |
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Principal Investigator |
SHIBAHARA Hiroaki Jichi Medical School, Obstetrics & Gynecology, Associate Professor, 医学部, 助教授 (80206143)
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| Project Period (FY) |
2000 – 2002
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| Keywords | GPI-anchored protein / Sperm surface protein / Sperm-immobilizing antibody / Fertilization / Embryo development / 疾患感受性遺伝子 |
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| Research Abstract |
It has been shown that the GPI (glycosylphosphatidyl-inositol)-anchored proteins on the surface of the mammalian cells play important roles. We have shown that PH-20 and SAGA-1 are the examples of the GPI-anchored proteins on the surface of human sperm by treating them with PI-PLC, a specific enzyme. By biotining the surface of human sperm, a couple of unknown GPI-anchored proteins have been identified using 2-D gel electrophoresis.
It was also suggested that a few number of GPI-anchored proteins associate with sperm-immobilization. In this study, we have identified the coordinates of 4 prominent immunoreactive sperm proteins, considered as possibly relevant to antibody mediated female infertility using 2-D gel electrophoresis.
Some infertile women having sperm-immobilizing antibodies were treated by IVF-ET. When patient's serum was supplemented in the culture media, fertilization and embryo development were significantly inhibited. The patient's serum containing human sperm-immobilizing antibodies also blocked fertilization and embryo development in mice.
These findings suggest that the immunocontraceptives could be developed using some sperm proteins including GPI-anchored proteins, that produce anti-sperm antibodies in women.
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