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2002 Fiscal Year Final Research Report Summary

Function of FGF-2 associated protein (FAP) and its application for preventing reclosure after glaucoma filtration surgery.

Research Project

Project/Area Number 12671726
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Ophthalmology
Research InstitutionKANAZAWA MEDICAL UNIVERSITY

Principal Investigator

YOSHITAKE Yosino  KANAZAWA MEDICAL UNIVERSITY, Medical School, Associate Professor, 医学部, 助教授 (00150764)

Co-Investigator(Kenkyū-buntansha) TAKAHASHI Nobuo  KANAZAWA MEDICAL UNIVERSITY, Medical School, Professor, 医学部, 教授 (20006787)
FUKUDA Masamichi  KANAZAWA MEDICAL UNIVERSITY, Medical School, Associate Professor, 医学部, 講師 (70208966)
Project Period (FY) 2000 – 2002
Keywordsglaucoma / postoperative care / growth factor / FGF / microtubules / yeast two-hybrid assay / mitosis / cytoskeleton
Research Abstract

FAP has been found as FGF associated protein in the course of the study on the function of high molecular weight form of FGF-2 in nuclei by yeast two-hybrid screening. FAP gene shares sequence similarity with an uncharacterized cDNA reported in GenBank as KIAA0092. FAP contains 500 amino acids and has the motifs homologous to that of tropomyosin to form the coiled-coil structure at N-terminal side. The protein also contains 3 nuclear localizing signals at C-terminal side. GFP-FAP fusion protein over-expressed in HeLa cells was predominantly observed in filamentous structure and co-localized with microtubules (MT). To confirm MT binding and bundling of the protein, HeLa cells expressing GFP-FAP was treated with vinblastine to disassemble the MT network. Vinblastine was not able to disrupt MT networks in the cells over-expressing GFP-FAP. Weekly-expressed tagged FAP fusion proteins in HeLa cells appeared to be co-localized with MT as spindle, spindle pole and MT in midbody in the mitotic cells. Observations of expression pattern of various deletion mutants of FAP in the cells showed that a half of C-terminal is MT binding domain. Yeast two-hybrid assay showed that FAP also has a strong self-associating activity. These results indicate that FAP is self-assembling protein at least partly associating with microtubule. The protein may control the mitotic machinery. Intracellular interaction between FAP and high molecular weight form of FGF-2 in nuclei is still unclear.

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Yoshitake, Y., Tiffani L., Howard, Jan L.Christian, Stanley M.Hollenberg: "Misexpression of polycomb-group proteins in Xenopus alters anterior neural development and represses neural target genes"Dev. Biol.. 215(2). 375-387 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Oda, M.: "Cell injury effect of isopropyl unoprostone, an anti-glaucoma agent, on cultured human conjunctive cells"J. Ocul. Pharmacol. Ther.. 15(6). 489-496 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Fukuda, M.: "Studies on the binding mechanism of fluoroquinones to melanin"J. Infect. Chemother.. 6(2). 72-76 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shui, Y.B., Sasaki, H., Pan J.H., Hata, I., Kojima, M., Yamada, Y., Hirai, K.I., Takahashi, N., Sasaki, K.: "Morphological observation on cell death and phagocytosis induced by ultraviolet irradiation in a cultured human lens epithelial cell line"Exp. Eye Res.. 71(6). 609-618 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sato, J., Segami, N., Suzuki, T., Yoshitake, Y., Nishikawa, K.: "The expression of fibroblast growth factor-2 and fibroblasts growth factor receptor-1 in chondrocytes in synovial chondromatosis of the temporomandibular joint"INT. J. Oral Maxillofac. Surg.. 31. 532-536 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Fukuda, M., Sasaki, K.: "General purpose antimicrobial ophthalmic solutions evaluated using new pharmacokinetic paramester of maximum drug concentration in aqueous"JAP. J. OPHTHALMOL.. 46. 384-390 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yoshitake, Y., Tiffani L. Howard, Jan L. Christian, and Stanley M. Hollenberg: "Misexpression of polycomb-group proteins in Xenopus alters anterior neural development and represses neural target genes."Dev. Biol.. 215(2). 375-387 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Oda, M.: "Cell injury effect of isopropyl unoprostone, an anti-glaucoma agent, on cultured human conjunctive cells."J. Ocul. Pharmacol. Ther.. 15(6). 489-496 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Fiukuda, M.: "Studies on the binding mechanism of fluoroquinones to melanin."J. Infect. Chemother.. 6(2). 72-76 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shui, Y. B., Sasaki, H., Pan J. H., Hata, I., Kojima, M., Yamada, Y., Hirai, K. I., Takahashi, N., Sasaki, K.: "Morphological observation on cell death and phagocytosis induced by ultraviolet irradiation in a cultured human lens epithelial cell line."Exp. Eye Res.. 71(6). 609-618 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sato, J., Segami, N., Suzuki, T., Yoshitake, Y., Nishikawa, K.: "The expression of fibroblast growth factor-2 and fibroblasts growth factor receptor-1 in chondrocytes in synovial chondromatosis of the temporomandibular joint."INT. J. Oral Maxillofac. Surg.. 31. 532-536 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Fukuda, M., Sasaki, K.: "General purpose antimicrobial ophthalmic solutions evaluated using new pharmacokinetic paramester of maximum drug concentration in aqueous."JAP. J. Ophthalmol.. 46. 384-390 (2002)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2004-04-14  

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