2002 Fiscal Year Final Research Report Summary

Mechanism of ischemia-reperfusion injury

Research Project

Project/Area Number	12671756
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Plastic surgery
Research Institution	Osaka Medical College
Principal Investigator	UEDA Kouichi Osaka Medical College Faculty of Medicine Associate Professor, 医学部, 助教授 (90257858)
Co-Investigator(Kenkyū-buntansha)	YOSHIDA Ryotaro Osaka Medical College Faculty of Medicine Associate Professor, 医学部, 助教授 (10124760)
Project Period (FY)	2000 – 2002
Keywords	ischemia-reperfusion / macropharge / Dichloromethylene diphosphonate / rat skin flaps
Research Abstract	The purpose of this study is to evaluate whether macropharge is related to ischemia-reperfusion injury. The concern of macropharge was investigated by immuno-histochemical study in the ischemia-reperfusion model of rat skin flaps. The femoral artery and vein were clamped above and below the epigastric vessels for 11 h and then the clamp was released. Histological examination 12 h after reperfusion showed macropharge invasion into the flap and leukocyte invasion was recognized 24 h after reperfusion. Peroxidase activity in the flap presented a peak 48 h after reperfusion. Dichloromethylene diphosphonate (DMDP) was administered intravenously just before elevation of the right abdominal epigastric flap. However the effect by DMDP to ischemia-reperfusion injury was not recognized.

(1 results)