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2001 Fiscal Year Final Research Report Summary

The Role of Peroxisomes in Bone Formation

Research Project

Project/Area Number 12671812
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Functional basic dentistry
Research InstitutionKyushu Dental College

Principal Investigator

FUJIWARA Satoko  Dept. of Dentistry, Kyushu Dental College, Associate Professor, 歯学部, 助教授 (20047806)

Co-Investigator(Kenkyū-buntansha) NOGUCHI Tomoo  Dept. of Dentistry, Kyushu Dental College, Professor, 歯学部, 教授 (30073688)
Project Period (FY) 2000 – 2001
KeywordsPeroxisomes / Catalase / MC3T3-E1 cell / Uricase / Epiphyseal cartilage / D-aspartate oxidase / D-amino acid oxidase / Sucrose density gradient centrifugation
Research Abstract

1. The morphology, distribution and biological function of peroxisomes in the fetal bovine articular cartilage of 5-month-old.
Sucrose density gradient centrifugation of the homogenate from chondrocytes of the fetal bovine cartilage was performed.
(1) The fetal bovine cartilage contained normal size peroxizomes with density of about 1.25, although it had been reported that the tissues out of liver and kidney contained microperoxisomes with low density.
(2) Alanine : glyoxylate aminotransferase(AGT) which is only animotransferase in animal peroxisomes and is wide distributed in mammalian liver. AGT activity was not detected in peroxisomes of the fetal bovine cartilage, suggested that the function of peroxisomes in cartilage was different from liver.
(3) D-aspartate oxidase(D-Asp O) was localized in peroxisomes in the fetal bovine cartilage, suggested that D-saparate was present in this tissue and D-Asp O was related to the developmental processes.
2. The morphology, distribution and biological function of peroxisomes in MC3T3-E1 cell (osteoblast- like cell derived from mouse).
Catalase activity was detected in the homogenate of MC3T3-E1 cells, suggested that peroxisomes were present in MC3T3-E1 cell. Other peroxisomal marker enzyme activities were not detected in the homogenate. Therefore, the proliferation of peroxisomes by clofibrate (peroxisomal proliferator) treatment and some peroxisomal enzyme activities must be investigated further.

  • Research Products

    (15 results)

All Other

All Publications (15 results)

  • [Publications] Masuda, W.: "A new type of allantoinase in amphybian liver"Biosci. Biotechnol. Biochem.. 65. 2558-2560 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Maki, K.: "A new type of Matrix vesicles is found in fetal bovine tracheal cartilage"Conective Tissue Research. 41(2). 109-115 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hayashi, S.: "Evolution of urate-degrading enzymes in animal peroxisomes"Cell Biochem. Biophys.. 32. 123-129 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Masuda, W.: "ADP-ribosyl cyclase in rat salivary glands"Biochim. Biophys. Res. Commum.. 270. 469-472 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sakuraba, H.: "Metabolism of glyoxylate, the end product of purine degradation in liver peroxisomes of freshwater fish"Biochim. Biophys. Res. Commum.. 228. 603-606 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Fujiwara, S.: "Degradation of purines: only ureidoglycollate lyase out of degradingenzymes is present in mammals"Biochem. J.. 312. 315-318 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Noguchi, T.: "Comparative Biochemistry of Animal Peroxisomes"Research Signpost. 13 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Masuda W., Fujiwara S. and Noguchi T.: "A new type of allantoinase in amphybian liver"Biosci. Biotechnol. Biochem.. 65 (11). 2558-2560 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Maki K., Hayashi S., Nishioka T., Kimura M. and Noguchi T.: "A new type of matrix vesicles is found in fetal bovine tracheal cartilage"Conective Tissue Research. 41 (2). 109-115 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Masuda W. and Noguchi T.: "ADP-ribosyl cyclase in rat salivary glands"Biochim. Blophys. Res. Commum.. 270. 469-472 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hayashi S., Fujiwara S. and Noguchi T.: "Evolution of urate-degrading enzymes in animal peroxisomes"Cell Biochem. Biophys. 32. 123-129 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sakuraba H., Fujiwara S. and Noguch T.: "Metabolism of glyoxylate, the end product of purine degradation in liver peroxisomes of freshwater fish"Biochim. Biophys. Res. Commum.. 229. 603-606 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Fujiwara S. and Noguchi T.: "Degradation of purines : only ureidoglycollate lyase out of degrading enzymes is present in mammals"Biochem. J.. 312. 315-318 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Noguchi T., Fujiwara S., Hayashi S., and Sakuraba H.: "Amino acid and glyoxylate metabolism"Research Signpost, 1-7, 1997 Comparative Biochemistry of Animal Peroxisomes.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Noguchi T., Fujiwara S., Hayashi S. and Sakuraba H.: "Purine metabolism"Research Signpost, 8-13, 1997 Comparative Biochemistry of Animal Peroxisomes.

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2003-09-17  

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