2001 Fiscal Year Final Research Report Summary
The Role of Peroxisomes in Bone Formation
| Project/Area Number |
12671812
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Functional basic dentistry
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| Research Institution | Kyushu Dental College |
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Principal Investigator |
FUJIWARA Satoko Dept. of Dentistry, Kyushu Dental College, Associate Professor, 歯学部, 助教授 (20047806)
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| Co-Investigator(Kenkyū-buntansha) |
NOGUCHI Tomoo Dept. of Dentistry, Kyushu Dental College, Professor, 歯学部, 教授 (30073688)
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| Project Period (FY) |
2000 – 2001
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| Keywords | Peroxisomes / Catalase / MC3T3-E1 cell / Uricase / Epiphyseal cartilage / D-aspartate oxidase / D-amino acid oxidase / Sucrose density gradient centrifugation |
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| Research Abstract |
1. The morphology, distribution and biological function of peroxisomes in the fetal bovine articular cartilage of 5-month-old.
Sucrose density gradient centrifugation of the homogenate from chondrocytes of the fetal bovine cartilage was performed.
(1) The fetal bovine cartilage contained normal size peroxizomes with density of about 1.25, although it had been reported that the tissues out of liver and kidney contained microperoxisomes with low density.
(2) Alanine : glyoxylate aminotransferase(AGT) which is only animotransferase in animal peroxisomes and is wide distributed in mammalian liver. AGT activity was not detected in peroxisomes of the fetal bovine cartilage, suggested that the function of peroxisomes in cartilage was different from liver.
(3) D-aspartate oxidase(D-Asp O) was localized in peroxisomes in the fetal bovine cartilage, suggested that D-saparate was present in this tissue and D-Asp O was related to the developmental processes.
2. The morphology, distribution and biological function of peroxisomes in MC3T3-E1 cell (osteoblast- like cell derived from mouse).
Catalase activity was detected in the homogenate of MC3T3-E1 cells, suggested that peroxisomes were present in MC3T3-E1 cell. Other peroxisomal marker enzyme activities were not detected in the homogenate. Therefore, the proliferation of peroxisomes by clofibrate (peroxisomal proliferator) treatment and some peroxisomal enzyme activities must be investigated further.
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