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2002 Fiscal Year Final Research Report Summary

Efficient development of new Japanese pear cultivars with high functions by genetic engineering methods

Research Project

Project/Area Number 12794002
Research Category

Grant-in-Aid for University and Society Collaboration

Allocation TypeSingle-year Grants
Research Field 園芸・造園学
Research InstitutionTottori University

Principal Investigator

TANABE Kenji  Faculty of Agriculture, Piofessor, 農学部, 教授 (40032106)

Co-Investigator(Kenkyū-buntansha) OTANI Hiroshi  Faculty of Agriculture, Professor, 農学部, 教授 (50032305)
ITAI Akihiro  Faculty of Agriculture, Associate Professor, 農学部, 助教授 (10252876)
TAMURA Fumio  Faculty of Agriculture, Professor, 農学部, 教授 (50217197)
TABIRA Hiroki  Tottori Horticultural Research Center, Researcher, 生物工学研究室, 室長(研究職)
KODAMA Motoichiro  Faculty of Agriculture, Assistant professor, 農学部, 講師 (00183343)
Project Period (FY) 2000 – 2002
KeywordsStorage ability / Self-compatibilty / Black spot resistance / Pear scab resistance / Gene diagnosis / Ethylene
Research Abstract

Tottori University researchers succeeded to release two self-compatible cultivars, 'Akibae' and 'Zuishu', which were resistant to black spot disease and had high quality. Moreover they released an early maturing cultivar 'Shinju', which were also resistant to black spot disease and had high quality. These cultivars already have been registered, and are open to public. Especially the cultivar, 'Akibae' deserved high reputation and its cultivated area increases to 40ha in 2003. They crossed these cultivars with 'Kinchaku', which is resistant to pear scab to produce new cultivars with resistance to pear scab. Shelf life of Japanese pear fruit is correlated with maximum ethylene production. They succeeded to develop the gene diagnosis for storage ability in Japanese pear cultivars and strains by analysis of genes for ethylene biosynthetic pathway. This diagnosis makes it possible to release new cultivars with high shelf life. With regard to mechanisms of black spot disease, two candidate receptor proteins for AK toxin from Alternaria alternata were identified. A trial for the gene cloning of the AK receptor protein is in progress. Researchers of Tottori Horticultural Research Center developed the gene diagnosis for self-compatibility. This also makes it easy to find new cultivars without artificial pollination.

  • Research Products

    (30 results)

All Other

All Publications (30 results)

  • [Publications] Itai, A., T.Kotaki, K.Tanabe, F.Tamura, O.Kawaguchi, M.Fukuda: "Rapid identification of 1-aminocyclopropane-1-carboxylate (ACC) synthase genotypes in cultivars of Japanese pear (Pyrus pyrifolia Nakai) using CAPS markers"Theoretical and Applied Genetics. 106. 1266-1272 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Itai, A., K.Tanabe, F.Tamura, M.Tomomitsu: "Cloning and characterization of a cDNA encoding 1-aminocyclopropane-1-carboxylate (ACC) synthase (PPACS3) from ripening fruit of Japanese pear (Pyrus pyrifolia Nakai)"Journal of Japanese Society for Horticultural Science. 72. 99-106 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Itai, A., K.Tanabe, F.Tamura: "The mechanism of ethylene production during fruit ripening in Asian pears"Acta Horticulturae. 587. 497-504 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Itai, A., K.Yoshida, K.Tanabe, F.Tamura: "Expression analysis of JPRXYL gene during fruit ripening in several pear cultivars"Acta Horticulturae. 596. 183-186 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Teng, Y., K.Tanabe, F.Tamura, A.Itai, A.: "Genetic relationships of Pyrus species and cultivars native to east Asia revealed by randomly amplified polymorphic DNA markers"Journal of the American Society for Horticultural Science. 127. 262-270 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Otani, H., M.Kodama, K.Kohmoto: "Infection mechanism of black spot pathogen in Japanese pear"Acta Horticulturae. 587. 623-630 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Singh, P., Nakajima, H., Otani, H., Kodama, M., Kohmoto, K., Park, R, Bugiani, R., Cavanni: "Host-specific SV-toxin of the fungal pathogen causing brown spot of European pear"Acta Horticulturae. 596. 507-511 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tamura, F., K.Tanabe, A.Itai: "Regulation of endodormancy in Japanese pear"Acta Horticulturae. 587. 325-336 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Teng, Y., K.Tanabe, F.Tamura, A.Itai, A.: "Assessment of genetic relatedness among large-fruited pear cultivars native to east Asia using RAPD markers"Acta Horticulturae. 587. 139-146 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Teng, Y.K.Tanabe, F.Tamura, A.Itai, A.: "Genetic relationships of pear cultivars in Xinjiang ; China, as measured by RAPD markers"Journal of Horticultural Science and Biotechnology. 76. 771-779 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Johnson, L.J., Johnson, R.D., Akamatsu, H., Salamiah, A., Otani, H., Kohmoto, K., Kodama, M.: "Spontaneous loss of a conditionally dispensable chromosome from the Alternaria alternata apple pathotype leads to loss of toxin production and pathogenicity."Current Genetics. 40. 65-72 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tamura, F., K.Tanabe, A.Itai, M.Morimoto: "Variation in the chilling requirement for breaking leaf bud endodorrnancy in wild pear species and pear cultivars"Journal of Japanese society for Horticultural Science. 70. 596-598 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Itai, A., K.Tanabe, F.Tamura, T.Tanaka: "Isolation of cDNA clones corresponding to the genes expressed during fruit ripening in Japanese pear (Pyrus pyritolia Nakai) -Involvement of ethylene signal transduction pathway in their expression"Journal of Experimental Botany. 51. 1163-1166 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Singh, R, Park, R, Bugiani, R., Cavanni, P., Nakajima, H., Kodama, M., Otani, H., Kohmoto, K.: "Effects of host-selective SV-toxin from Stemphylium vesicarium, the cause of brown spot of European pear plants, on ultrastructure of leaf cells"Journal of Phytopathology. 148. 87-93 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Otani, H.: "Host recognition by plant pathogens and role of host-specific toxins"Journal of General Plant Pathology. 66. 278-280 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Itai, A., T.Kotaki., K.Tanabe, F.Tamura, D.Kawaguchi and M.Fukuda.: "Rapid identification of 1-aminocyclopropane-1-carboxylate (ACC) synthase genotypes in cultivars of Japanese pear (Pyrus pyrifolia Nakai) using CAPS markers"Theor. Appl. Genet.. 106. 1266-1272 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Itai, A., K.Tanabe, F.Tamura and M.Tomomitsu: "Cloning and characterization of a cDNA encoding 1-aminocyclopropane-1- carboxylate (ACC) synthase (PRAC33) from ripening fruit of Japanese pear (Pyrus pyrifolia Nakai)"J. Japan. Soc. Hart. Sci.. 72. 99-106 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Itai, A., K.Tanabe and F.Tamura: "The mechanism of ethylene production during fruit ripening in Asian pears"Acta Hort.. 587. 497-504 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Itai, A., K.Yoshida, K.Tanabe and F.Tamura: "Expression analysis of JPRXVL gene during fruit ripening in several pear cultivars"Acta Hort.. 596. 183-186 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Teng, Y., K.Tanabe, F.Tamura and A.Itai: "A Genetic relationships of Pyrus species and cultivars nativeeast Asia revealed by randomly amplified polymorphic DNA markers"J. Amer. Soc. Hort. Sci.. 127. 262-270 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Otani, H., M.Kodama and K.Kohmoto: "Infection mechanism of black spot pathogen in Japanese pear"Acta. Hort.. 587. 623-630 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Singh, P., Nakajima, H., Otani, H., Kodama, M., Kohmoto, K., Park, P., Bugiani, R., and Cavanni: "Host-specific SV-toxin of the fungal pathogen causing brown spot of European pear"Acta Hort.. 596. 507-511 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tamura,F., K.Tanabe and A.Itai: "Regulation of endodormancy in Japanese pear"Acta. Hort.. 587. 325-336 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Teng, Y., K.Tanabe, F.Tamura and A.Itai: "A Assessment of genetic relatedness among large-fruited pear cultivars native to east Asia using RAPD markers"Acta Hort.. 587. 139-146 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Teng, Y., K.Tanabe, F.Tamura and A.Itai: "A Genetic relationships of pear cultivars in Xinjiang ; China, as measured by RAPD markers"J. Hort. Sci. Biotech.. 76. 771-779 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Johnson, L.J., Johnson, R.D., Akamatsu, H., Salamiah, A., Otani, H., Kohmoto, K. and Kodama, M: "Spontaneous loss of a conditionally dispensable chromosome from the Altemaria alternata apple pathotype leads to loss of toxin production and pathogenicity"Curr. Genet.. 40. 65-72 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tamura, F., K,Tanabe, A.Itai and M.Morimoto: "Variation in the chilling requirement tor breaking leaf bud endodorrnancy in wild pear species and pear cultivars"J. Japan. Soc. Hort. Sci.. 70. 596-598 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Itai, A., K.Tanabe, F.Tamura and T.Tanaka: "Isolation of cDMA clones corresponding to the genes expressed during fruit ripening in Japanese pear (Pyrus pyrifolia Nakai) -Involvement of ethylene signal transduction pathway in their expression"J. Exp. Bot.. 51. 1163-1166 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Singh, P., Park, P., Bugiani, R., Cavanni, P., Nakajima, H., Kodama, M., Otani, H. and Kohmoto, K: "Effects of host-selective SV-toxin from Stemphylium vesicarium, the cause of brown spot of European pear plants, on ultrastructure of leaf cells"J. Phytapath. 148. 87-93 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Otani, H.: "Host recognition by plant pathogens and role of host-specific toxins"J.General Plant Pathol.. 66. 278-280 (2000)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2004-04-14  

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