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2001 Fiscal Year Final Research Report Summary

Conversion of animal food and resources wastes into functional food materials

Research Project

Project/Area Number 12839010
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 生物資源の変換と展開
Research InstitutionHIROSHIMA UNIVERSITY

Principal Investigator

NISHIMURA Toshihide  Hiroshima University, Faculty Applied Biological Science, Professor, 生物生産学部, 教授 (70180643)

Co-Investigator(Kenkyū-buntansha) TANABE Soichi  Hiroshima University, Faculty Applied Biological Science, Associate Professor, 生物生産学部, 助教授 (90272624)
Project Period (FY) 2000 – 2001
KeywordsProtection of environment / Utilization of animal and food and resources waste / Yeast / Fish scraps / DHA / Animal blood / Functional peptide / Hypothesive activity
Research Abstract

This study was done to utilize animal food and resources wastes, such as fish scraps in supermarkets and blood in a slaughterhouse. Especially, we thought their conversion into functional food materials.
Some yeasts that grew using fish oil as a carbon source were selected. Among them, a yeast F0726A was shown to incorporate an eicosapentaenoic acid (EPA) and a docosahexaenoic acid in its cell. When grown at 20 ℃ for 24 h in an optimal medium containing 1 g scrap fish oil, the yeast incorporated 23.2 mg EPA and 54.8 mg DHA in 0.82 g dry cells. The recovery rates of EPA and DHA from fish oil were 27.1 and 43.6% respectively. Geotrichum sp. F0347-2 was also shown to incorporate a DHA in its cell. It accumulated 53 mg DHA in 1 g dry cells after cultivation at 30 ℃ for 24 h. These yeasts seem to be very useful for incorporation of EPA and DHA possessing good physical function.
A hydrolysate of hemoglobin, a major protein in blood, was prepared by the treatment of proteases. This hydrolysate exhibited hypotensive activity in a spontaneously hypertensive rat (SHR), when this hydrolysate (1g/kg weight of rat) was orally administered into SHR. The blood pressure declined at 4 hr after admistration. Peptides involved in hypotensive activity were isolated on a HPLC by ODS column, and sequenced. Five peptides, TPAVH, HLDDDLK, VAAAL, VDPVNF and VVYPW, were identified. These peptides synthesized on the basis of structures possessed angiotensin I-converting enzyme inhibitory activity. This hydrolysate may be useful as a functional food material.

  • Research Products

    (4 results)

All Other

All Publications (4 results)

  • [Publications] X.Guo, Y.Ota: "Incorporation of eicosapentaenoic and dodosahexaenoic acids by a yeast(F0726A)"J.Applied Microbiology. 89. 107-115 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Kinoshita, Y.Ota: "Concentration of Docosahexaenoic Acid from Fish Oils Using Geotrichum sp.F0347-2"Biosci.Biotechnol.Biochem.. 65. 1022-1026 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] X. Guo, and Y. Ota: "Incorporation of eicosapentaenoic and docosahexaenoic acids by a yeast (F0726A)"J. Applied Microbiology. 89. 107-115 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H. Kinoshita, and Y. ota: "Concentration of docosahexaenoic acid from fish oils using Geotrichum sp. F0347-2"Biosci. Biotechnol. Biochem. 65. 1022-1026 (2001)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2003-09-17  

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