2001 Fiscal Year Final Research Report Summary
Conversion of animal food and resources wastes into functional food materials
| Project/Area Number |
12839010
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
生物資源の変換と展開
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| Research Institution | HIROSHIMA UNIVERSITY |
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Principal Investigator |
NISHIMURA Toshihide Hiroshima University, Faculty Applied Biological Science, Professor, 生物生産学部, 教授 (70180643)
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| Co-Investigator(Kenkyū-buntansha) |
TANABE Soichi Hiroshima University, Faculty Applied Biological Science, Associate Professor, 生物生産学部, 助教授 (90272624)
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| Project Period (FY) |
2000 – 2001
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| Keywords | Protection of environment / Utilization of animal and food and resources waste / Yeast / Fish scraps / DHA / Animal blood / Functional peptide / Hypothesive activity |
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| Research Abstract |
This study was done to utilize animal food and resources wastes, such as fish scraps in supermarkets and blood in a slaughterhouse. Especially, we thought their conversion into functional food materials.
Some yeasts that grew using fish oil as a carbon source were selected. Among them, a yeast F0726A was shown to incorporate an eicosapentaenoic acid (EPA) and a docosahexaenoic acid in its cell. When grown at 20 ℃ for 24 h in an optimal medium containing 1 g scrap fish oil, the yeast incorporated 23.2 mg EPA and 54.8 mg DHA in 0.82 g dry cells. The recovery rates of EPA and DHA from fish oil were 27.1 and 43.6% respectively. Geotrichum sp. F0347-2 was also shown to incorporate a DHA in its cell. It accumulated 53 mg DHA in 1 g dry cells after cultivation at 30 ℃ for 24 h. These yeasts seem to be very useful for incorporation of EPA and DHA possessing good physical function.
A hydrolysate of hemoglobin, a major protein in blood, was prepared by the treatment of proteases. This hydrolysate exhibited hypotensive activity in a spontaneously hypertensive rat (SHR), when this hydrolysate (1g/kg weight of rat) was orally administered into SHR. The blood pressure declined at 4 hr after admistration. Peptides involved in hypotensive activity were isolated on a HPLC by ODS column, and sequenced. Five peptides, TPAVH, HLDDDLK, VAAAL, VDPVNF and VVYPW, were identified. These peptides synthesized on the basis of structures possessed angiotensin I-converting enzyme inhibitory activity. This hydrolysate may be useful as a functional food material.
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