2000 Fiscal Year Annual Research Report

RNA研究の21世紀への展開

Research Project

Project/Area Number	12898020
Research Institution	The University of Tokyo
Principal Investigator	中村義一東京大学, 医科学研究所, 教授 (40114590)
Co-Investigator(Kenkyū-buntansha)	横山茂之東京大学, 大学院・理学系研究科, 教授 (00159229) 渡辺公綱東京大学, 大学院・新領域創成科学研究科, 教授 (00134502) 志村令郎東京大学, 生物分子工学研究所, 所長(研究職) (60025426) 饗場弘二名古屋大学, 大学院・理学研究科, 教授 (20025662) 多比良和誠東京大学, 大学院・工学系研究科, 教授 (10261778)
Keywords	RNA / ネットワーク / RNA蛋白質複合体 / 高次複合機能 / 分子擬態 / ポストゲノム / 情報発現 / 翻訳調節
Research Abstract	近年のRNA研究の大きな発展を支えた背景には、国内のRNAの基礎研究に関して10年余の間継続して実施されてきたRNAに関係する重点・特定領域研究が果たした役割が大きい。このようなRNA研究に対する熱意は、特に若い研究者の間に大きなうねりとなって現れ、平成11年に日本RNA学会が組織された(初代会長:志村令郎・生物分子工学研究所長)。本基盤研究(C)においては、「RNA研究の21世紀への展開」のために推進すべき課題についての調査と討論を重ね、平成13年度発足特定領域研究(A)「RNA情報発現系の時空間ネットワーク」を申請するに至った。その過程で、本基盤研究(C)に参加し、同時に新特定領域研究の総括班に予定するメンバーは、平成12年に開催されたRNA関連の国際研究集会「tRNA Workshop」(4月、ケンブリッジ)、「RNA Society年会」(5月、マジソン)、FASEBシンポジウム「Posttranscriptional Control of Gene Expression:The Role of RNA」(7月、コロラド)、「Ribosome Biogenesis」(8月、タホ)、「Structural Aspects of Protein Synthesis」(9月、アルバニ)に参加し、最新情報の調査・討論を行った。これらの調査討論に基づき新特定領域研究の申請を準備するとともに、平成13年2月には、国外から12名の研究者を招聘して国際シンポジウム「Post-Genome World of RNA」(開催責任者:東京大学医科学研究所教授中村義一)を東京で開催し、本研究領域に関する最新の発表・討論を実施した。

Research Products

(15 results)

All Other

All Publications (15 results)

[Publications] Ito,K.: "A tripeptide 'anticodon' deciphers stop codons in messenger RNA."Nature. 403. 680-684 (2000)
[Publications] Nakamura,Y.: "Mimicry grasps reality in translation termination."Cell. 101. 349-352 (2000)
[Publications] Wilson,K.: "Functional sites of interaction between release factor RF1 and the ribosome."Nature Struct.Biol.. 7. 866-870 (2000)
[Publications] Tanaka,R.: "Intronic U50 small-nucleolar-RNA (snoRNA) host gene of no protein-coding potential is mapped at the chromosome breakpoint t(3;6)(q27;q15) of human B-cell lymphoma"Genes Cells. 5. 277-287 (2000)
[Publications] Tin,O.F.: "Proteolytic fragmentation of polypeptide release factor 1 of Thermus hermophilus and crystallization of the stable fragments."Biochimie. 82. 765-772 (2000)
[Publications] Toyoda,T.: "Crystal structure combined with genetic analysis of the Thermus thermophilus ribosome recycling factor shows that a flexible hinge may act as a functional switch."RNA. 6. 1432-1444 (2000)
[Publications] Ishino,T.: "Interaction of ribosome recycling factor and elongation factor EF-G with E.coli ribosomes studied by surface plasmon resonance technique."Genes Cells. 5. 953-963 (2000)
[Publications] Fujiwara,T.: "Functional mapping of the ribosome contact site in the ribosome recycling factor."RNA. 7. 64-70 (2001)
[Publications] Abo,T.: "SsrA-mediated tagging and proteolysis of LacI and its role in the regulation of lac operon."EMBO J.. 19. 3762-3769 (2000)
[Publications] Tanaka,Y: "A novel regulatory role of glucose transporter of Escherichia coli : membrane sequestration of a global repressor Mlc."EMBO J.. 19. 5344-5352 (2000)
[Publications] Sekine,S.: "Structural basis for anticodon recognition by discriminating glutamyl-tRNA synthetase."Nature Struct.Biology. (In Press).
[Publications] Sakamoto,S.: "Molecular Computation by DNA Hairpin Formation."Science. 288. 1223-1226 (2000)
[Publications] Fukai,S.: "Structural basis for double-sieve discrimination or L-valine from L-isoleucine and L-threonine by the complex of tRNA (Val) and valyl-tRNA synthetase"Cell. 103. 793-803 (2000)
[Publications] Itoh,T.: "Role of the ENTH domain in phosphatidylinositol-4,5-bisphosphate binding and endocytosis."Science. 291. 1047-1051 (2001)
[Publications] Nakamura,Y.: "Genetic probes to bacterial release factors : tRNA mimicry hypothesis and beyond.In : The Ribosorne : Structure, Function, Antibiotics and Ceuular Interactions"The American Society for Microbiology, Washington, D.C.. 519-526 (2000)

2000 Fiscal Year Annual Research Report

RNA研究の21世紀への展開

Principal Investigator

中村 義一 東京大学, 医科学研究所, 教授 (40114590)

Research Products

[Publications] Ito,K.: "A tripeptide 'anticodon' deciphers stop codons in messenger RNA."Nature. 403. 680-684 (2000)

[Publications] Nakamura,Y.: "Mimicry grasps reality in translation termination."Cell. 101. 349-352 (2000)

[Publications] Wilson,K.: "Functional sites of interaction between release factor RF1 and the ribosome."Nature Struct.Biol.. 7. 866-870 (2000)

[Publications] Tanaka,R.: "Intronic U50 small-nucleolar-RNA (snoRNA) host gene of no protein-coding potential is mapped at the chromosome breakpoint t(3;6)(q27;q15) of human B-cell lymphoma"Genes Cells. 5. 277-287 (2000)

[Publications] Tin,O.F.: "Proteolytic fragmentation of polypeptide release factor 1 of Thermus hermophilus and crystallization of the stable fragments."Biochimie. 82. 765-772 (2000)

[Publications] Toyoda,T.: "Crystal structure combined with genetic analysis of the Thermus thermophilus ribosome recycling factor shows that a flexible hinge may act as a functional switch."RNA. 6. 1432-1444 (2000)

[Publications] Ishino,T.: "Interaction of ribosome recycling factor and elongation factor EF-G with E.coli ribosomes studied by surface plasmon resonance technique."Genes Cells. 5. 953-963 (2000)

[Publications] Fujiwara,T.: "Functional mapping of the ribosome contact site in the ribosome recycling factor."RNA. 7. 64-70 (2001)

[Publications] Abo,T.: "SsrA-mediated tagging and proteolysis of LacI and its role in the regulation of lac operon."EMBO J.. 19. 3762-3769 (2000)

[Publications] Tanaka,Y: "A novel regulatory role of glucose transporter of Escherichia coli : membrane sequestration of a global repressor Mlc."EMBO J.. 19. 5344-5352 (2000)

[Publications] Sekine,S.: "Structural basis for anticodon recognition by discriminating glutamyl-tRNA synthetase."Nature Struct.Biology. (In Press).

[Publications] Sakamoto,S.: "Molecular Computation by DNA Hairpin Formation."Science. 288. 1223-1226 (2000)

[Publications] Fukai,S.: "Structural basis for double-sieve discrimination or L-valine from L-isoleucine and L-threonine by the complex of tRNA (Val) and valyl-tRNA synthetase"Cell. 103. 793-803 (2000)

[Publications] Itoh,T.: "Role of the ENTH domain in phosphatidylinositol-4,5-bisphosphate binding and endocytosis."Science. 291. 1047-1051 (2001)

[Publications] Nakamura,Y.: "Genetic probes to bacterial release factors : tRNA mimicry hypothesis and beyond.In : The Ribosorne : Structure, Function, Antibiotics and Ceuular Interactions"The American Society for Microbiology, Washington, D.C.. 519-526 (2000)

中村義一東京大学, 医科学研究所, 教授 (40114590)