2004 Fiscal Year Final Research Report Summary
a study on isolation and clonal expansion of the stem cell of hepatic stellate cells.
| Project/Area Number |
13470001
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
General anatomy (including Histology/Embryology)
|
|---|
| Research Institution | Akita University |
|---|
Principal Investigator |
SENOO Haruki Akita University, School of Medicine, Professor, 医学部, 教授 (90171355)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
SATO Mitsuru Akita University, School of Medicine, Associate professor, 医学部, 助教授 (60226008)
IRIE Toshiaki Akita University, School of Medicine, Research associate, 医学部, 助手 (90231167)
HIGASHI Nobuyo Akita University, School of Medicine, Research associate, 医学部, 助手 (60361218)
|
|---|
| Project Period (FY) |
2001 – 2004
|
| Keywords | stellate cell / liver / stem cell / liver bud / septum transversum / foregut / clone / liver diverticulun |
|---|
| Research Abstract |
A stem cell of the hepatic stellate cells was established from mouse embryos. We observed morphologically the process of liver bud formation mixing mesenchymal tissue and foregut in the mouse embryos. During 10-14 somite stage, the hepatic diverticulum was formed and during 18-25 somite stage the foregut entered the septum transversum and the liver bud formation was started. Cell masses obtained from the liver bud containing the hepatic diverticulum and the septum transversum were quickly removed and treated with dispase to get the suspension of isolated cells. The isolated cells were cultured in (1)polystyrene culture dishes, (2)gelatin-coated polystyrene dishes, or (3)polystyrene dishes with a feeder layer of mouse embryo fibroblasts. Three culture media were used : (1)DMEM high glucose medium containing 10% calf serum, (2)DMEM/F12 medium containing 15% calf serum, and (3)ES cell growing medium containing 15% calf serum. To inhibit cell differentiation, a leukemia inhibiting factor (1,000 u/ml) was added to each medium. The best condition was obtaining with ES cell growing medium in gelatin-coated polystyrene dishes. After getting colonies in the dish, each colony was cloned. Cell type was identified by immunofluorescence by using specific antibody against BMP4, AFP, or FLT1(VEGFR), and each antibody demonstrated stellate cells, parenchymal cells, and endothelial cells, respectively. Thus, we established the stem cell of the hepatic stellate cells. The same clone was obtained from flt1 knock out mouse embryos.
|
-
-
-
-
[Journal Article] Uptake of collagen into hepatic stellate cells : Evidence of the involvement of urokinase plasminogen activator associated protein/Endo 180.2004
Author(s)
Mousavi, S.A., Sato, M., Sporstol, M., Smedsrod, B., Berg, T., Senoo, H
-
Journal Title
Biochem.J. October(on line published)
Description
「研究成果報告書概要(欧文)」より
-
[Journal Article] Vitamin A distribution and content in tissues of the lamprey (Lampetra japonica).2004
Author(s)
Wold, H.L., Wake, K., Higashi, N., Wang, D-R., Kojima, N., Imai, K., Blomhoff, R., Senoo, H.
-
Journal Title
Anat Rec 276A
Pages: 134-142
Description
「研究成果報告書概要(欧文)」より
-
-
[Journal Article] Role of hepatic stellate cells in the early phase of liver regeneration by formation of tight adhesion to parenchymal cells in rat.2004
Author(s)
Mabuchi, A., Mullaney, I., Sheard, P., Hessian, O., Zimmermann, A., Senoo, H., Wheatley, T.M.
-
Journal Title
J Hepatol. 40
Pages: 1023-1026
Description
「研究成果報告書概要(欧文)」より
-
-
-
-
[Journal Article] Stimulation of pro-MMP-2 production and activation by native form of extracellular type I collagen in cultured hepatic stellate cells.2003
Author(s)
Wang, D.-R., Sato, M., Li, L.-L., Miura, M., Kojima, N., Senoo, H.
-
Journal Title
Cell Struct.Func. 28
Pages: 505-513
Description
「研究成果報告書概要(欧文)」より
-
-
-
