2002 Fiscal Year Final Research Report Summary
The roles of cell adhesion molecules in the metastasis-related gene network downstream of HOXD3 gene
| Project/Area Number |
13470048
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Experimental pathology
|
|---|
| Research Institution | HOKKAIDO UNIVERSTY |
|---|
Principal Investigator |
HAMADA Jun-ichi Hokkaido Univ., Institute for Genetic Medicine, Associate Professor, 遺伝子病制御研究所, 助教授 (50192703)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
TADA Mitsuhiro Hokkaido Univ., Institute for Genetic Medicine, Associate Professor, 遺伝子病制御研究所, 助教授 (10241316)
MORIUCHI Tetsuya Hokkaido Univ., Institute for Genetic Medicine, Professor, 遺伝子病制御研究所, 教授 (20174394)
|
|---|
| Project Period (FY) |
2001 – 2002
|
| Keywords | homeobox gene / HOXD3 / metastasis / TGF-beta / integrin beta3 / invasion / motility |
|---|
| Research Abstract |
We have been studying the mechanisms of tumor invasion and metastasis from the viewpoint of abnormal expression of homeobox genes by tumor cells. We previously found that the overexpression of HOXD3 gene, one of the homeobox genes converted human lung cancer A5 49 cells into more motile, invasive and metastatic ones. We also revealed the altered expressions of metastasis-related genes such as integrin beta3, MMP-2, uPA and E-cadherin in A549 cells overexpressing HOXD3 gene. In this study, we examined the roles of cell adhesion molecules in the metastasis-related gene network downstream of the HOXD3 gene. We obtained the following results from this study. 1) Enhanced motility of A549 cells by the HOXD3-overexpression was dependent on integrin alphavbeta3 ; 2) the expressions of 15 of 30 genes of which expressions were altered by the HOXD3-overexpression were similarly changed by the signals through integrin alphavbeta3 ; 3) the HOXD3-overexpression converted latent TGF-beta into active one which activated the sigunaling pathways through TGF-beta receptors in an autocrine manner ; 4) TGF-beta stimulated the in vitro invasion and migration of A549 cells to type I collagen ; 5) TGF-beta induced the similar expression patterns in 9 of 30 metastasis-related genes of which expressions were altered by the HOXD3-overexpression.
|
