2003 Fiscal Year Final Research Report Summary
The role of sex steroid on the generation of sex differences in the brain development with special attention to androgen.
| Project/Area Number |
13470335
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Urology
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| Research Institution | Ehime University |
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Principal Investigator |
TANAKA Junya EHIME UNIV., MEDICINE, PROFESSOR, 医学部, 教授 (70217040)
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| Co-Investigator(Kenkyū-buntansha) |
HAPA Ryuji EHIME UNIV., MEDICINE, ASSOCIATE PROFESSOR, 医学部, 助教授 (90258153)
YOKOYAMA Masayoshi EHIME UNIV., MEDICINE, PROFESSOR, 医学部, 教授 (50116993)
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| Project Period (FY) |
2001 – 2003
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| Keywords | MICROGLIA / ESTOSTERONE / AQUAPORIN / ASTROCYTES / NEURON / APOLIPOPROTEINE / L-Ser / L-Ser |
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| Research Abstract |
1)Microglia and astrocytes were found to express receptors for androgen as revealed by RT-PCR study.
2)Based on comprehensive experiments with RT-PCR technique, testosterone was found to increase the expression of mRNA encoding aquaporin 4 in astrocytes. Not only testosterone but progesterone and dehydrotestosterone also upregulated the expression of aquaporin 4 at both mRNA and protein level. Protein kinase C (PKC) activator phorbol ester abolished the testosterone-induced increase of aquaporin 4. This suggests that the action of androgen is dependent on the action of PKC.
3) Microglia of mesodermal origin have prosperities resembling monocytes/macrophages. We have found that microglia possess a nature as a multipotential neural stem cells. By incubating microglial cells in culture medium containing 70% serum, the cells came to have a nature as undifferentiated stem cells. The stem-like microglia differentiated into neurons or oligodendrocytes after they were transferred into serum-free medium. Testosterone enhanced the transformation of microglial cells into cells with neuroectodermal phenotypes through the undifferentiated cells. Furthermore, testosterone altered the expression pattern of mRNAs encoding GFAP.
4)Microglial cells were found to produce and release lipoproteins containing apolipoprotein E, cholesterol, phospholipids and triglycerides in the presence of L-Ser. Testosterone elicited inhibitory effects on the release of lipoproteins by microglial cells.
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[Publications] Y Kuwabara, A Yokoyama, L Yang, K Toku, K Mori, I Takeda, T Shigekawa, B Zhang, N Maeda, M Sakanaka, Junya Tanaka: "Two populations of microglial cells isolated from rat primary mixed glial cultures."Journal of Neuroscience Research. 73. 22-30 (2003)
Description
「研究成果報告書概要(和文)」より
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[Publications] N.Ohkubo, Y.-D.Lee, A.Morishima, T.Terasaka, S.Kikkawa, M.Tohyama, M.Sakanaka, J.Tanaka, N.Maeda, M.P.Vitek, N.Mitsuda: "Apolipoprotein E and reelin ligands modulate tau phosphorylation through an apolipoprotein E receptor/disabled-a/glycogen synthase kinase-3b cascade."FASEB Journal. (Internet Journal). 10.1096/fj.02-0434fje (2002)
Description
「研究成果報告書概要(和文)」より
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[Publications] Hiroki Sugishita, Yasuhide Kuwabara, Kazuko Toku, Lisa Doi, Lihua Yang, Junya Mitoma, Shigeki Furuya, Yoshio Hirabayashi, Nobuji Maeda, Masahiro Sakanaka, Junya Tanaka: "L-Serine regulates the activities of microglial cells that express very low level of 3-phosphoglycerate dehydrogenase, an enzyme for L-serine biosynthesis."Journal of Neuroscience Research. 64. 392-401 (2001)
Description
「研究成果報告書概要(和文)」より
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[Publications] Y Kuwabara, A Yokoyama, L Yang, K Toku, K Mori, I Takeda, T Shigekawa, B Zhang, N Maeda, M Sakanaka, Junya Tanaka: "Two populations of microglial cells isolated from rat primary mixed glial cultures."Journal of Neuroscience Research. 73. 22-30 (2003)
Description
「研究成果報告書概要(欧文)」より
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[Publications] N.Ohkubo, Y.-D.Lee, A.Morishima, T.Terasaka, S.Kikkawa, M.Tohyama, M.Sakanaka, J.Tanaka, N.Maeda, M.P.Vitek, N.Mitsuda: "Apolipoprotein E and reelin ligands modulate tau phosphorylation through an apolipoprotein E receptor/disabled-a/glycogen synthase kinase-3b cascade."FASEB Journal. 10.1096/fj(Internet Journal). 02fje-0434fje (2002)
Description
「研究成果報告書概要(欧文)」より
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[Publications] K.Mon, E.Ozaki, B.Zhang, L.Yang, A.Yokoyama, I.Takeda, N.Maeda, M.Sakanaka, J.Tanaka: "Effects of norepinephrine on rat cultured microglial cells that express α1,α2,β1 and β2 adrenergic receptors."Neuropharmacology. 43. 1026-1034 (2002)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Hiroki Sugishita, Yasuhide Kuwabara, Kazuko Toku, Lisa Doi, Lihua Yang, Junya Mitoma, Shigeki Furuya, Yoshio Hirabayashi, Nobuji Maeda, Masahiro Sakanaka, Junya Tanaka: "L-Serine regulates the activities of microglial cells that express very low level of 3-phosphoglycerate dehydrogenase, an enzyme for L-serine biosynthesis."Journal of Neuroscience Research. 64. 392-401 (2001)
Description
「研究成果報告書概要(欧文)」より
