2003 Fiscal Year Final Research Report Summary
An effective analysis of individual cells using flow cytometry and its clinical applicatjion.
| Project/Area Number |
13470516
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | The University of Tokyo |
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Principal Investigator |
NAKAHARA Kazuhiko The University of Tokyo, Faculty of Medicine, Professor, 医学部附属病院, 教授 (70101095)
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| Co-Investigator(Kenkyū-buntansha) |
YONEYAMA Akiko The University of Tokyo, Faculty of Medicine, Lecturer, 大学院・医学系研究科, 寄附講座教員 (50175684)
HIGASHI Katsumi The University of Tokyo, Faculty of Medicine, Assistant Professor, 医学部附属病院, 助手 (50159109)
WATANABE Takashi Kyorin University, Faculty of Medicine, Associate Professor, 医学部, 教授 (00191768)
IKEDA Tadako Kyorin University, Faculty of Medicine, Lecturer, 医学部, 講師 (90099242)
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| Project Period (FY) |
2001 – 2003
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| Keywords | flow cytometry / cell surface antigen / antigen molecule density / cell size / FF index / quantification of cell surface molecule / hematological malignancy / clinical application |
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| Research Abstract |
The purpose of this study is to explore an effective application of flow cytometry (FCM) to clinical medicine by using the information of individual cells. Until now, cytometric immunophenotyping using FCM has focused mainly on the quantification of the proportion of cells bearing certain combinations of molecules and the change of fluorescence intensity (FI) dose not reflect the antigen density directly. For the qualification of cell surface molecule density, the forward light scatter (FSC) and FI, obtained by FCM on an individual cell basis were plotted in a scattergram where x-and y-axis show FSC and FI respectively. Where a proportional relationship between FI and FSC can been seen, the slope of the line of regression (or its absolute value) may be used as an indicator of the molecule density, which is referred to as the FF index. In this way, the FI vs FSC scattergram enables the evaluation of the relationship between the size of the cell and the amount of antigen expressed. Additionally we explored the computer software which enables to practice automatically these analysis by using the listmode of FCM. We analyzed normal peripheral lymphocytes, hematological malignant cells and lymphocytes of hemodyalisis patients and found the potent usefulness of more precise clinical analysis using this software. In future, more clinical studies, the evaluation and standardization of the developed software and actual quantification of cell surface antigens are needed.
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