2004 Fiscal Year Final Research Report Summary
Phylogenetic aspects of the cyclostomes with special attention to the primitive complement sysytem.
| Project/Area Number |
13640700
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
系統・分類
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| Research Institution | Hiroshima Prefectural Women's University |
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Principal Investigator |
FUJII Tamotsu Hiroshima Prefectural Women's University, Faculty of Human Life and Environmental Science, Professor, 生活科学部, 教授 (10181314)
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| Co-Investigator(Kenkyū-buntansha) |
SUGAWARA Yoshiaki Hiroshima Prefectural Women's University, Faculty of Human Life and Environmental Science, Professor, 生活科学部, 教授 (30154462)
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| Project Period (FY) |
2001 – 2004
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| Keywords | Complement / Innate immunity / Lectin pathway / Cyclostome / Hagfish / C3 convertase / MASP / Serine protease |
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| Research Abstract |
Hagfish is the most, primitive extant vertebrate and thought to defend itself against pathogens only by innate immune system. With a view to understanding the evolution of the innate immune system, we have isolated complement C3 from the serum of hagfish. In this study, our attempt to identify a hagfish C3 convertase was carried out by RT-PCR using degenerative primers designed on the basis of the conserved amino acid stretches among the several kinds of serine proteases. Analyses of a full-length cDNA clone isolated from a hagfish liver cDNA library using one of the cloned RT-PCR products as a probe indicated that this cDNA encoded hagfish mannose-binding lectin-associated serine protease WASP)1. This evidence strongly suggests that the hagfish defends itself against pathogens at least by the complement system composed of lectin pathway
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Research Products
(6 results)
