2002 Fiscal Year Final Research Report Summary
Molecular analysis of rye-drived midget chromosomes by microdissection
| Project/Area Number |
13660007
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Breeding science
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| Research Institution | Okayama University |
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Principal Investigator |
MURATA Minoru Okayama Univ., Res. Inst. Bioresour., Prof., 資源生物科学研究所, 教授 (20166292)
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| Co-Investigator(Kenkyū-buntansha) |
OGURA Yutaka Okayama Univ., Res. Inst. Bioresour., Assist. Prof., 資源生物科学研究所, 助手 (60224193)
SAKAMOTO Wataru Okayama Univ., Res. Inst. Bioresour., Assoc. Prof., 資源生物科学研究所, 助教授 (20222002)
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| Project Period (FY) |
2001 – 2002
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| Keywords | rye / microdissection / wheat / chromosome / cytoplasmic male sterility / DP-PCR / midget |
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| Research Abstract |
The midget chromosomes in (cereale)-Chinese Spring were microdissected by UV-laser, and the DNA was amplified by using DOP (Degenerate Oligonucleotide-Primed)-PCR. Fluorescence in situ and genomic Southern hybridizations clearly showed that the amplified DNA originated from the midget chromosome. Sequence analysis revealed that low -copy sequences as well as retrotransposon-like and minisatellite sequences were involved in the amplified DNA. In from the midget chromosomes. Some of them had relatively high homologies to those of known genes, but none seems to be related to the mitochondrial and/or chloroplastic functions.
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