2002 Fiscal Year Final Research Report Summary

Molecular analysis of rye-drived midget chromosomes by microdissection

Research Project

Project/Area Number	13660007
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Breeding science
Research Institution	Okayama University
Principal Investigator	MURATA Minoru Okayama Univ., Res. Inst. Bioresour., Prof., 資源生物科学研究所, 教授 (20166292)
Co-Investigator(Kenkyū-buntansha)	OGURA Yutaka Okayama Univ., Res. Inst. Bioresour., Assist. Prof., 資源生物科学研究所, 助手 (60224193) SAKAMOTO Wataru Okayama Univ., Res. Inst. Bioresour., Assoc. Prof., 資源生物科学研究所, 助教授 (20222002)
Project Period (FY)	2001 – 2002
Keywords	rye / microdissection / wheat / chromosome / cytoplasmic male sterility / DP-PCR / midget
Research Abstract	The midget chromosomes in (cereale)-Chinese Spring were microdissected by UV-laser, and the DNA was amplified by using DOP (Degenerate Oligonucleotide-Primed)-PCR. Fluorescence in situ and genomic Southern hybridizations clearly showed that the amplified DNA originated from the midget chromosome. Sequence analysis revealed that low -copy sequences as well as retrotransposon-like and minisatellite sequences were involved in the amplified DNA. In from the midget chromosomes. Some of them had relatively high homologies to those of known genes, but none seems to be related to the mitochondrial and/or chloroplastic functions.

(4 results)