2002 Fiscal Year Final Research Report Summary
Biosynthesis of phytotoxins in phytopathogenic fungi
| Project/Area Number |
13660108
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Bioproduction chemistry/Bioorganic chemistry
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| Research Institution | Tottori University |
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Principal Investigator |
NAKAJIMA Hiromitsu Tottori University, Faculty of Agriculture, Professor, 農学部, 教授 (40144646)
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| Project Period (FY) |
2001 – 2002
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| Keywords | Phytopathogens / Neocosmospora vasinfecta / reduction enzyme / phytotoxins / SDS-PAGE / biosynthesis / native PAGE / purification |
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| Research Abstract |
Neovasifuranones are phytotoxins produced by the phytopathogenic fungus Neocosmospora vasinfecta NHL2298. Recently conversion of neovasifuranone A aldehyde into neovasifuranone A was observed in the cell-free system obtained from this fungus. We studied on the properties of this of this conversion enzyme(s)and found that there are two enzymes involved in this conversion step. We determined the molecular weight, optimal pH, optimal temperature, Km and Vmax values of these two enzymes. We also purified one of these enzymes by centrifugation, DE-52,Sephacryl S-300, TSKgel G3000SW and Mono Q column chromatographies. The purest sample showed one major protein band around 40kDa on SDS-PAGE analysis. This band protein was determined to be an enzyme protein after native PAGE and enzyme assay.
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