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2003 Fiscal Year Final Research Report Summary

Epidemiological study on viral zoonotic diseases originating from wild rodents in Japan

Research Project

Project/Area Number 13660311
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied veterinary science
Research InstitutionHokkaido University

Principal Investigator

KARIWA Hiroaki  Hokkaido Univ., Grad.School of Vet.Med., Assoc, Prof., 大学院・獣医学研究科, 助教授 (70224714)

Co-Investigator(Kenkyū-buntansha) ARIKAWA Jiro  Hokkaido Univ., Grad.School of Med., Prof., 大学院・医学研究科, 教授 (10142704)
MIZUTANI Tetsuya  National Inst., Infectious Diseases, Researcher, 主任研究員 (70281681)
TAKASHIMA Ikuo  Hokkaido Univ., Grad.School of Vet.Med., Prof., 大学院・獣医学研究科, 教授 (30002083)
FUKUSHIMA Hiroshi  Shimane Prefectural Institute of Public Health and Environment Science, Chief Researcher, 感染症科, 主任研究員
TUCHIYA Kimiyuki  Tokyo Univ., Agriculture, Faculty of Agriculture, Prof., 農学部, 教授 (30155402)
ANDO Shuji  Toyama Institute of Health, Chief Researcher
Project Period (FY) 2001 – 2003
Keywordszoonosis / rodent / epidemiology / hemorrhagic fever with renal syndrome / tick-borne encephalitis
Research Abstract

To know the epidemiology of viral zoonotic diseases such as hemorrhagic fever with renal syndrome (HFRS) and tick-borne encephalitis (TBE) in Japan, we conducted an epizootiological survey in various points of Japan. Anti-hantavirus antibodies were detected in Apodemus. speciosus (5/482), and Clethnonomys rufocanus (7/197), Rattus norvegicus (4/364), and Rattus rattus (3/45) by Indirect fluorescent antibody assay (IFA). The positive sera from A.speciosus neutralized Hantaan virus at 1:20 but did not neutralize Seoul virus. This indicate that hantavirus carried by A.speciosus is closer to Hantaan virus than Seoul virus. The partial S gene was amplified from seropositive R.rattus and sequenced. The virus sequence had 96% identitiy with SR-11 which is Seoul virus prototype strain. These results suggest that main reservoir animals of hantavirus in Japan are A.speciosus, C. rufocanus, R.norvegicus, and R rattus.
The rodent sera were applied to the newly established ELISA by using recombinant E protein of TBE virus to detected anti-TBE virus antibodies. The ELISA could detect anti-TBE antibodies in sera positive by neutralization test. However, no antibodies were detected in rodent sera from Toyama prefecture, in which TBE infections has not confirmed. These results suggest that the ELISA can be a useful method to identify the endemic areas of TBE in Japan.

  • Research Products

    (44 results)

All Other

All Publications (44 results)

  • [Publications] Takashima I, et al.: "Epidemiology of tick-borne encephalitis (TBE) and phylogenetic analyasis of TBE viruses in Japan and Far Eastern Russia."Jpn J Infect Dis.. 54. 1-11 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Arikawa J, et al.: "Epidemiology and epizootiology of hantavirus infection in Japan."Jpn J Infect Dis.. 54(3). 95-102 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Araki K, et al.: "Truncated hantavirus nucleocapsid proteins for serotyping Hantaan, Seoul, and Dobrava hantavirus tions."J Clin Microbiol.. 39. 2397-2404 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hayasaka D, et al.: "Distribution and characterization of tick-borne encephalitis viruses from Siberia and far-eastern Asia."J Gen Virol.. 82. 1319-1328 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hayasaka D, et al.: "Evaluation of European tick-borne encephalitis virus vaccine against recent Siberian and far-eastern subtype strains."Vaccine. 19. 4774-4779 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kariwa H, et al.: "Epizootiological survey of hantavirus among rodent species in Ningxia Hui Autonomous Province, China."Jpn J Vet Res.. 49. 105-114 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Goto A., et al.: "Genetic and biological comparison of tick-borne encephalitis viruses from Hokkaido and far-eastern Russia."Jpn J Vet Res.. 49. 297-307 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Lokugamage K, et al.: "Genetic characterization of hantaviruses transmitted by the Korean field mouse (Apodemus peninsulae), Far East Russia."Emerg Infect Dis.. 8. 768-776 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yoshii K, et al.: "Enzyme-linked immunosorbent assay using recombinant antigens expressed in mammalian cells for serodiagnosis of tick-borne encephalitis."J Virol Methods.. 108. 171-179 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Araki K, et al.: "Hantavirus-specific CD8(+)-T-cell responses in newborn mice persistently infected with Hantaan virus."J Virol.. 77. 8408-8417 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Miyamoto H, et al.: "Serological analysis of hemorrhagic fever with renal syndrome (HFRS) patients in Far Eastern Russia and identification of the causative hantavirus genotype."Arch Virol.. 148. 1543-1556 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kariwa H, et al.: "Synthesis of Seoul virus RNA and structural proteins in cultured cells."Arch Virol.. 148(9). 1671-1685 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kariwa H.: "Bunyavirus- relationship between virus and host : Coevolution of hantavirus and rodent."Virus. 52. 61-67 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Lee BH, et al.: "Detection of antibody for the serodiagnosis of hantavirus infection in different rodent species."Arch Virol.. 148. 1885-1897 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Lokugamage N, et al.: "Development of an efficient method for recovery of Puumala and Puumala-related viruses by inoculation of Mongolian gerbils."J Vet Med Sci.. 65. 1189-1194 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Lokugamage K, et al.: "Comparison of virulence of various hantaviruses related to hemorrhagic fever with renal syndrome in newborn mouse model."Jpn J Vet Res.. 51. 143-149 (2004)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Araki K, et al.: "A new model of Hantaan virus persistence in mice : the balance between HTNV infection and CD8+ T-cell responses."Virology. 322(2). 318-327 (2004)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Araki K, et al.: "Age-dependent hantavirus-specific CD8+ T-cell responses in mice infected with Hantaan virus."Arch Virol.. (In press). (2004)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hayasaka D, et al.: "Amino acid changes responsible for attenuation of virus neurovirulence in an infectious cDNA clone of the Oshima strain of tick-borne encephalitis virus."J Gen Virol.. 85. 1007-1018 (2004)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Iwasa MA, et al.: "Modes of hantavirus transmission in a population of Clethrionomys rufocanus bedfordiae inferred from mitochondrial and microsatellite DNA analyses."Arch Virol.. 149(5). 929-941 (2004)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kariwa H.: "Epizootiological of hantavirus infections."BAMSA J.. 15. 16-22 (2004)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kariwa H.: "Hemorrhagic fever with renal syndrome."Sustainable Livestock Production and Human Welfare. 58. 42-45 (2004)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takashima I, Hayasaka D, Goto A, Kariwa H, Mizutani T.: "Epidemiology of tick-borne encephalitis (TBE) and phylogenetic analysts of TBE viruses in Japan and Far Eastern Russia."Jpn J Infect Dis.Review.. 54. 1-11 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Arikawa J, Yoshimatsu K, Kariwa H.: "Epidemiology and epizootiology of hantavirus infection in Japan."Jpn J Infect Dis.Review.. 54(3). 95-102 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Araki K, Yoshimatsu K, Ogino M, Ebihara H, Lundkvist A, Kariwa H, Takashima I, Arikawa J.: "Truncated hantavirus nucleocapsid proteins for serotyping Hantaan, Seoul, and Dobrava hantavirus tions."J Clin Microbiol.. 39. 2397-2404 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hayasaka D, Ivanov L, Leonova GN, Goto A, Yoshii K, Mizutani T, Kariwa H, Takashima I.: "Distribution and characterization of tick-borne encephalitis viruses from Siberia and far-eastern Asia."J Gen Virol.. 82. 1319-1328 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hayasaka D, Goto A, Yoshii K, Mizutani T, Kariwa H, Takashima I.: "Evaluation of European tick-borne encephalitis virus vaccine against recent Siberian and far-eastern subtype strains."Vaccine.. 19. 4774-4779 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kariwa H, Zhong CB, Araki K, Yoshimatsu K, Lokugamage K, Lokugamage N, Murphy ME. Mizutani T, Arikawa J, Fukushima H, Xiong H, Jiehua C, Takashima I.: "Epizootiological survey of hantavirus among rodent species In Ningxia Hui Autonomous Province, China."Jpn J Vet Res.. 49. 105-114 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Goto A, Hayasaka D, Yoshii K, Mizutani T, Kariwa H, Takashima I.: "Genetic and biological comparison of tick-borne encephalitis viruses from Hokkaido and far-eastern Russia."Jpn J Vet Res.. 49. 297-307 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Lokugamage K, Kariwa H, Hayasaka D, Cui BZ, Iwasaki T, Lokugamage N, Ivanov LI, Volkov VI, Demenev VA, Slonova R, Kompanets G, Kushnaryova T, Kurata T, Maeda K, Araki K, Mizutani T, Yoshimatsu K, Arikawa J, Takashima I.: "Genetic characterization of hantaviruses transmitted by the Korean field mouse (Apodemus peninsulae), Far East Russia."Emerg Infect Dis.. 8. 768-776 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yoshii K, Hayasaka D, Goto A, Obara M, Araki K, Yoshimatsu K, Arikawa J, Ivanov L, Mizutani T, Kariwa H, Takashima I.: "Enzyme-linked immunosorbent assay using recombinant antigens expressed in mammalian cells for serodiagnosis of tick-borne encephalitis."J Virol Methods.. 108. 171-179 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Araki K, Yoshimatsu K, Lee BH, Kariwa H, Takashima I, Arikawa J.: "Hantavirus-specific CD8(+)-T-cell responses in newborn mice persistently infected with Hantaan virus."J Virol.. 77. 8408-8417 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Miyamoto H, Kariwa H, Araki K, Lokugamage K, Hayasaka D, Cui BZ, Lokugamage N, Ivanov LI, Mizutani T, Iwasa MA, Yoshimatsu K, Arikawa J, Takashima I.: "Serological analysis of hemorrhagic fever with renal syndrome (HFRS) patients in Far Eastern Russia and identification of the causative hantavirus genotype."Arch Virol.. 148. 1543-1556 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kariwa H, Tanabe H, Mizutani T, Kon Y, Lokugamage K, Lokugamage N, Iwasa MA, Hagiya T, Araki K, Yoshimatsu K, Arikawa J, Takashima I.: "Synthesis of Seoul virus RNA and structural proteins in cultured cells."Arch Virol.. 148(9). 1671-1685 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kariwa H.: "Bunyavirus-relationship between virus and host : Coevolutlon of hantavirus and rodent."Virus(In Japanese). 52. 61-67 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Lee BH, Yoshimatsu K, Araki K, Ogino M, Okumura M, Tsuchiya K, Kariwa H, Arikawa J.: "Detection of antibody for the serodiagnosis of hantavirus infection in different rodent species."Arch Virol.. 148. 1885-1897 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Lokugamage N, Kariwa H, Lokugamage K, Hagiya T, Miyamoto H, Iwasa MA, Araki K, Yoshimatsu K, Arikawa J, Mizutani T, Takashima I.: "Development of an efficient method for recovery of Puumala and Puumala-related viruses by inoculation of Mongolian gerbils."J Vet Med Sci.. 65. 1189-1194 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Lokugamage K, Kariwa H, Lokugamage N, Iwasa M, Hagiya T, Araki K, Tachi A, Mizutani T, Yoshimatsu K, Arikawa J, Iwasaki T, Takashima I.: "Comparison of virulence of various hantaviruses related to hemorrhagic fever with renal syndrome in newborn mouse model."Jpn J Vet Res.. 51. 143-149 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Araki K, Yoshimatsu K, Lee BH, Kariwa H, Takashima I, Arikawa J.: "A new model of Hantaan virus persistence in mice the balance between HTNV infection and CD8+ T-cell responses."Virology. 322(2). 318-327 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Araki K, Yoshimatsu K, Lee BH, Okumura M, Kariwa H, Takashima I, Arikawa J.: "Age-dependent hantavirus-specific CDB+ T-cell responses in mice infected with Hantaan virus."Arch Virol.. (In press). (2004)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hayasaka D, Gritsun TS, Yoshii K, Ueki T, Goto A, Mizutani T, Kariwa H, Iwasaki T, Gould EA, Takashima I.: "Amino acid changes responsible for attenuation of virus neurovirulence in an infectious cDNA clone of the Oshima strain of tick-borne encephalitis virus."J Gen Virol.. 85. 1007-1018 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Iwasa MA, Kariwa H, Cui BZ, Lokugamage K, Lokugamage N, Hagiya T, Mizutani T, Takashima I.: "Modes of hantavirus transmission in a population of Clethrionomys rufocanus bedfordiae inferred from mitochondrial and microsatellite DNA analyses."Arch Virol.. 149(5). 929-941 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kariwa H.: "Epidemiology of hantavirus infections."BAMSA J.(In Japanese). 15. 16-22 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kariwa H.: "Hemorrhagic fever with renal syndrome."Sustainable Livestock Production and Human Welfare(In Japanese). 58. 42-45 (2004)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2005-04-19  

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