2002 Fiscal Year Final Research Report Summary
Topological analysis of GPI-80 in human neutrophils
| Project/Area Number |
13670022
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | Jichi Medical School |
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Principal Investigator |
MATSUBARA Shigeki Jichi Medical School, Medicine, Professor, 医学部, 教授 (20209597)
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| Co-Investigator(Kenkyū-buntansha) |
TAKAHASHI Katsumasa Gunma University, Medicine, Researcher, 医学部, 助手 (30326839)
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| Project Period (FY) |
2001 – 2002
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| Keywords | human neutrophils / GPI-80 / GPI-anchored protein / CD18 / intracellular granules / immunocytochemistry / cell biology |
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| Research Abstract |
GPI-80 is a novel GPI-anchored protein on human phagocytes that may regulate leukocyte adherence, locomotion, and extravasation. A unique intracellular compartment that contains GPI-80 is distinct from the previously characterized azurophil and specific granules. The GPI-80-containing compartments rapidly undergo a dramatic reorganization upon cell stimulation with a chemoattractant. By high-resolution immunofluorescence microscopy using ultrathin cryosections, after fMLP-stimulation, some GPI-80 molecules were co localized with beta 2 integrin molecules (CD18) on the cell surface. These results suggest that GPI-80 is physically associated with beta 2 integrin in human neutrophils.
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