2002 Fiscal Year Final Research Report Summary
Electrophysiological analysis of feeding-related network in the hypothalamus, based on the gluco-static and lipo-static theories
| Project/Area Number |
13670056
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Environmental physiology (including Physical medicine and Nutritional physiology)
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| Research Institution | Toyama University |
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Principal Investigator |
SASAKI Kazuo Toyama University, Toyama Univ., Fac.of Engineering, Lecturer (60042826)
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| Co-Investigator(Kenkyū-buntansha) |
BABA Yoshichika Toyama Univ., Fac.of engineering, Lecturer (30238232)
TSUKADA Akira Toyama National College of maritime Technology, Accociate Professor (40236849)
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| Project Period (FY) |
2001 – 2002
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| Keywords | Leptin / Ghrelin / Orexin / Arcuate nucleus / Rat |
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| Research Abstract |
1. In brain slice experiments, neuronal activity of the ventro-medial part of the arcuate nucleus (mARC) in rats was excited dose-dependently by application of leptin. The ratio of neurons excited by 10^<-10>M leptin was about 60%. The excitation was observed in low Ca^<2+>, high Mg^<2+> Ringer's solution as well as in normal Ringer's solution.
2. Neuronal activity of the mARC was excited dose-dependently by application of orexin-A. The ratio of mARC neurons excited by 10^<-7>M orexin-A was about 70%. The excitation was observed in low Ca^<2+>, high Mg^<2+> Ringer's solution as well as in normal Ringer's solution. Neurons excited by orexin-A were also excited by orexin-B. The magnitude of excitation induced by orexin-B was greater than that induced by orexin-A.
3. Neuronal activity of the mARC was excited dose-dependently by ghrelin. The ratio of mARC neurons excited by 10^<-7>M ghrelin was about 70%. The excitation was observed in low Ca^<2+>, high Mg^<2+> Ringer's solution as well as in normal Ringer's solution. About a half of neurons excited by ghrelin was also excited by orexin.
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