2003 Fiscal Year Final Research Report Summary
In vivo enhancement of antigen-specific antibody production through IL-5 and IL-13 in transgenic mice expressing GATA-3
| Project/Area Number |
13670473
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
内科学一般
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| Research Institution | Kitasato University |
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Principal Investigator |
TAMAUCHI Hidskazu Kitasato Univ., School of Medicine, Research Associate, 医学部, 助手 (60188414)
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| Project Period (FY) |
2001 – 2003
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| Keywords | GATA3 / Epsonophils / transgenic mice / IL-5 / IL-13 / EPO / asthma / 気道抵抗 |
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| Research Abstract |
The transcription factor GATA-3 has been shown to be dispensable for Th2 cell development from the naive CD4^+ T cells. To clarify how the in vivo immune response is governed under GATA3 function, we generated the double transgenic mice by crossing of GATA-3 transgenic and OVA-specific TCR transgenic mice. After i.p. immunization with OVA, the double transgenic (GATA-3/TCR-Tg) mice showed the increased GATA-3 expression in CD4^+ T cells and enhanced cytokine producing activity of IL-5 and IL-13 while IL-4 production was equally increased between the GATA-3/TCR-Tg and single TCR transgenic (WT/TCR-Tg)mice. The serum level of OVA antigen-specific IgG, IgE and IgA antibodies was also increased in the immunized GATA-3/TCR-Tg mice in comparison to WT/TCR-Tg mice. According to the increased IL-5, eosinophilic infiltration in the bronchial tracts become larger. These results clearly demonstrated that in vivo induction of Th2 cytokine producing cells in the immunized T cells as well as in vitro pattern, resulting in the antigen-specific antibody for three isotypes through IL-5 and IL-13, but not IL-4.
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