2002 Fiscal Year Final Research Report Summary
The Molecular Analysis of Trefoil Factors (TFF) in Gastroenterology
| Project/Area Number |
13670552
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | Dokkyo University School of Medicine |
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Principal Investigator |
TERANO Akira Dokkyo University, Department of Medicine, Professor, 医学部, 教授 (50155470)
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| Co-Investigator(Kenkyū-buntansha) |
TAMANO Masaya Dokkyo University, Department of Medicine, Lecturer, 医学部, 講師 (70265312)
SHIMADA Tadahito Dokkyo University, Department of Medicine, Associate Professor, 医学部, 助教授 (10206170)
HIRAISHI Hideyuki Dokkyo University, Department of Medicine, Associate Professor, 医学部, 助教授 (00199035)
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| Project Period (FY) |
2001 – 2002
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| Keywords | Trefoil Peptide / pS2 gene / rITF / stomach / colon / Peptic ulcer / GFP |
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| Research Abstract |
It is documented that Trefoil peptides (TP) play very important roles in various gastrointestinal diseases like colon polyp, colon cancer, inflammatory bowel diseases.
This peptide consists of pS2 gene, rITF and other peptides. TPs are located in the mucosa of GI tracts including stomach, small intestines as well as colon. In this project, we attempted to investigate the role of these peptides in the healing process of gastric mucosal damage and colonic damage. Several growth factors like EGF, TGFa, FGF and HGF are reported to promote the healing of GI tract mucosal injuries like peptic ulcers or Crohn's disease, UC. The effects of these peptides on the healing of gastric ulcer induced by NSAIDs in rat stomach were tested. Firstly the localization of rITF in rat stomach or intestine was investigated. rITF was located in the mucosa of rat stomach as well as intestine or colon. Further, this peptide was strongly expressed at the edge of the gastric ulcer on the healing stage. The immunostaining procedures were employed by using anti-rITF antibody. Moreover, we tested the effects of TP on the proliferation and migration of epithelial cells by using the monolayer culture of gastric mucosal cells. Consequently, TP strongly promoted the proliferation and migration of gastric mucosal cells in culture. For the future study, it is planned to study the molecular biological study of these peptides to elucidate the mechanisms of the action of repair process.
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