2003 Fiscal Year Final Research Report Summary
Transdifferentiation of bone marrow stromal cells in hippocampal slice culture model
| Project/Area Number |
13671435
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Cerebral neurosurgery
|
|---|
| Research Institution | Kobe University |
|---|
Principal Investigator |
KONDOH Takeshi Kobe University Hospital, Department of Neurosurgery, Associate Professor, 医学部附属病院, 講師 (50273769)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
KOHMURA Eiji Kobe University Graduate School of Medicine, Department of Neurosurgery, Professor, 大学院・医学系研究科, 教授 (30225388)
NISHIZAKI Tomoyuki Hyogo Medical College, Department of Physiology, Professor, 第二生理学教室, 教授 (00221474)
|
|---|
| Project Period (FY) |
2001 – 2003
|
| Keywords | Bone marrow stromal cells / brain grafting / slice culture / retinoic acid / neuronal plasticity |
|---|
| Research Abstract |
New possibilities for repairing the nervous system have arisen since the discovery of stem cells. Recent studies have demonstrated that different types of stem cells can be transplanted successfully in animal models. Adult green mice marrow stromal cells were co-cultured with hippocampal slices. Transdifferentiation to neuron-or glia-like cells occurred exclusively inside slice boundaries appearing at day 3, and then decreasing gradually over 35 days. Neuron-like cells tended to form network-like connections around day 14. The use of retinoic acid greatly enhanced the number of transdifferentiated cells and the best concentration was 10^<-6> M. NeuN Immunohistochemistry was positive in 9.6 ± 1.7% of morphologically transdifferentiated neuron-like cells. GFAP and lba1immunostainings were negative. We concluded that bone marrow stromal cells can be transdifferentiated into neurons, and direct contact with the host brain tissue is essential. Retinoic acid significantly increases the number of transdifferentiated cells as has been reported with other stem cells.
|
Research Products
(21 results)
-
-
-
-
[Publications] Tozaki, H., Kanno, T., Nomura, T., Kondou, T., Kosama, N., Saito, N., Aihara, H., Nagata, T., Matsumoto, S., Ohta, K., Nagai, K., Yajima, Y., Nishizaki, T.: "Role of glial glutamate transpoeters in the facilitatory action of FK960 on hippocampal neurotransmission"Brain Res Mol Brain Res. 97(1). 7-12 (2001)
Description
「研究成果報告書概要(欧文)」より
-
[Publications] Ueyama, T., Ren, Y., Sakai, N., Takahashi, M., Ono, Y., Kondoh, T., Tamaki, N., Saito, N.: "Generation of a constitutively active fragment of PKN in Microglia/macrophages after middle cerebral artery occlusion in rats"J Neurochem.. 79(4). 903-913 (2001)
Description
「研究成果報告書概要(欧文)」より
-
[Publications] Bhattacharjee AK., Kondoh, T., Nagashima, T., Ikesa, M., Ehara, K., Tamaki, K.: "Quantitative analysis of papaverine-mediated blood-brain barrier disruption in rats"Biochem Biophys Res Commun.. 289(2). 548-552 (2001)
Description
「研究成果報告書概要(欧文)」より
-
-
[Publications] Motooka, Y., Kondoh, T., Nomura, T., Tamaki, N., Tozaki, H., Kanno, T., Nishizaki, T.: "Selective cholinergic denervation inhibits expression of Long-term potentiation in the abult but not infant rat hippocampus"Brain Res Dev Brain Res.. 129(1). 119-123 (2001)
Description
「研究成果報告書概要(欧文)」より
-
-
-
[Publications] Tada, H., Uchino, M., Nomura, T., Kondoh, T., Saito, N., Yamamura, T., Yajima, Y., Nishizaki, T.: "The anti-dementia drug FK960 stimulates Glial glutamate release via a PKA pathway"Brain Res Mol Brain Res.. 109(1-2). 63-68 (2002)
Description
「研究成果報告書概要(欧文)」より
-
-
[Publications] Aihara, H., Fujiwara, S., Misuta, I., Tada, H., Kanno, T., Tozaki, H., Nagai, K., Yajima, Y., Inoue, K., Kondoh, T., Motooka, Y., Nishizaki: "Adenosine Triphosphate accelerates recovery from hypoxic/hypoglycemic perturbation of guinea pig hippocampal neurotransmission via a P(2) recepter"Brain Res. 952(1). 31-37 (2002)
Description
「研究成果報告書概要(欧文)」より
-
[Publications] Ohta, K., Nomura, T., Kanno, T., Nagaki, K., Yamamoto, S., Yajima, Y., Kondoh, T., Kohmura, E., Saito, N., Nishizaki, T: "L-trans-PDC enhances Hippocampal neuronal activity by stimulating glial glutamate release Independently of blocking transporters"Biochem Biophys Res Commun. 295(2). 376-381 (2002)
Description
「研究成果報告書概要(欧文)」より
-
[Publications] Nishizaki, T., Nagai, K., Nomura, T., Tada, H., Kanno, T., Tozaki, H., Li, XX., Kondoh, T., Kosama, N., Takahashi, E., Sakai, N., Tanaka, K., Saito, N.: "A new neuromodulatory pathway with a glial contribution mediated via A(2a) adenosine receptors"Glia. 39(2). 133-147 (2002)
Description
「研究成果報告書概要(欧文)」より
-
-
[Publications] Sakagami, Y., Okimura, Y., Kondoh, T., Iguchi, G., Fumoto, M., Kuno, T., Chihara, K., Kohmura, E.: "Expression of mPOU Protein in the Human Pituitary Adenomas"Kobe J Med Sci.. 49(5). 117-122 (2003)
Description
「研究成果報告書概要(欧文)」より
-
[Publications] Ikeda, M., Nagashima, T., Bhattacharjee AK., Kondoh, T., Kohrnura, E., Tamaki, N.: "Quantitative analysis of hyperosmotic and hypothermic Blood-brain barrier opening"Acta Neurochir Suppl.. 86. 559-563 (2003)
Description
「研究成果報告書概要(欧文)」より
-
[Publications] Hirano, M., Yamasaki, K., Sakurai, T., Kondoh, T., Ryu, Y., Okada, H., Sugimura, K., Kitazawa, S., Kitazawa, R., Maeda, S., Katafuchi, T., Tumura, S: "Measurement of blood vessel diameter for angiography using refraction contrast imaging"Igaku Butsuri. 23(2). 157-159 (2003)
Description
「研究成果報告書概要(欧文)」より
-
[Publications] Bhattacharjee, AK., Ueyama, T., Kondoh, T., Hayashi, S., Abouelfetouh, A., Sakai, N., Saito, N., Kohmura, E.: "In vivo transgene expression using an Adenoviral tetracycline-regulated system with neuron-specific enolase Promoter"Biochem Biophys Res Commun.. 317(4). 1144-1148 (2004)
Description
「研究成果報告書概要(欧文)」より
-
[Publications] Hirano, M., Yamasaki, K., Kitazawa, R., Kitazawa, S., Okada, H., Katafuchi, T., Sakurai, T., Kondoh, T., Ohbayashi, C., Maeda, S., Sugimura, K., Tamura, S.: "Imaging of fine structure of bone sample with high coherent X-ray beam and high spatial resolution detector"Radiat Med.. 22(1). 56-59 (2004)
Description
「研究成果報告書概要(欧文)」より
