2002 Fiscal Year Final Research Report Summary
A study regarding the mechanism in development of endolymphatic hydrops in Meniere's disease
| Project/Area Number |
13671797
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Otorhinolaryngology
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| Research Institution | Kitasato University |
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Principal Investigator |
NAGANUMA Hideaki Kitasato Univ. School of Medicine Assistant Professor, 医学部・耳鼻咽喉科学, 講師 (00198342)
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| Co-Investigator(Kenkyū-buntansha) |
KAWAHARA Katsumasa Kitasato Univ. School of Medicine Professor, 医学部・生理学, 教授 (70134525)
YAMASHINA Shohei Kitasato Univ. School of Medicine Professor, 医学部, 解剖学・教授 (90013987)
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| Project Period (FY) |
2001 – 2002
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| Keywords | Vasopressin / V-2receptor / endolymphatic hydrops / aquaporin / AQP-2 / dehydration / 脱水 |
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| Research Abstract |
Until now, the mechanism in development of endolymphatic hydrops was not elucidated. There were some reports that V-2receptor for Vasopressin and water channel aquaporin-2 (AQP-2) in inner ear membranous labyrinth participated in development of endolymphatic hydrops. It was confirmed that endolymphatic hydrops was developed morphologically in administering vasopressin to abdominal cavity in guinea pig in previous reports. It was not, however, confirmed that this animal model could reflect the diseases with endolymphatic hydrops including Meniere's disease.
In Study1, it was investigated whether the hearing disturbance was occurred in the endolymphatic hydrops animal model (Wistar rat) by administering vasopressin to abdominal cavity. As the results in Study1, it was confirmed that the hearing disturbance was occurred in 71.4%.
In Study2, the time course of the hearing disturbance in the endolymphatic hydrops animal model by administering vasopressin to abdominal cavity was investigated.
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As the results in Study2, it was confirmed as follows; 1) the hearing disturbance was occurred in 30-120 minutes after administering vasopressin, 2) the recovery in hearing was found in 50%, 3) the hearing disturbance was mainly in only one side (80%).
In Study3, the influence of dehydration load to the hearing disturbance in the endolymphatic hydrops animal model by administering vasopressin to abdominal cavity was investigated. As the results in Study3, in the endolymphatic hydrops animal model that dehydration for 24hrs was loaded, the hearing disturbance in only one side was occurred withn only 10 minutes after administering vasopressin. It was thought that AQP-2 was composed in the cell by dehydration load, AQP-2 moved to the cell membrane by the dosage of Vasopressin, water moved to endolymphatic cavity from the blood capillary and then the endolymphatic hydrops was occurred. It was suggested that dehydration load made the cells in membranous labyrinth get ready for the endolymphatic hydrops. Less
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