2002 Fiscal Year Final Research Report Summary

Inhabitation of type insulin-like growth factor receptor stimulates apoptosis induced by anticancer drugs in a human neurobustoma cell live without N-mycamplitication

Research Project

Project/Area Number	13671866
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Pediatric surgery
Research Institution	Osaka University
Principal Investigator	WASA Masafumi Osaka University Graduate School of Medicine, Associate Professor, 医学系研究科, 助教授 (10240467)
Co-Investigator(Kenkyū-buntansha)	KUSAFUKA Takeshi Osaka University Graduate School of Medicine, Associate Professor, 医学系研究科, 助手 (70263267)
Project Period (FY)	2001 – 2002
Keywords	Neuroblastoma / N-myc / IGF-1 receptor
Research Abstract	We investigated whether the blockage of IGF-I receptor (IGE-IR) by a monoclonal antibody against IGF-IR (αIR3) may be advantageously combined with conventional cytotoxic drugs for the design of more effective therapeutic regimens. Here, we used the well-characterized neuroblastoma cell line, SK-N-SH, that is known to express low level of N-myc amplification. Our findings indicate that combined treatment using αIR3 with cisplatin and doxorubicin significantly enhance in vitro cytotoxic effects of chemotherapeutic drugs by stimulating apoptotic process. Therapies that target the IGF-IR combined with traditional anticancer drugs should improve the effectiveness of the chemotherapy and probably even allow the usage of considerably lower doses of toxic drugs.