2002 Fiscal Year Final Research Report Summary
Basic research of gene therapy combination with radio, chemo and hyperthermic treatment on oral carcinoma
| Project/Area Number |
13672110
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | Nara Medical University |
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Principal Investigator |
KIRITA Tadashi Nara Medical University, Oral and Maxillofacial Surgery, Professor, 医学部, 教授 (70201465)
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| Co-Investigator(Kenkyū-buntansha) |
IMAI Yuichiro Nara Medical University, Oral and Maxillofacial Surgery, Research Associate, 医学部, 助手 (80347567)
OHNISHI Takeo Nara Medical University, Biology, Professor, 医学部, 教授 (60094554)
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| Project Period (FY) |
2001 – 2002
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| Keywords | Chemical Chaperone / Glycerol / Radiotherapy / Hyperthermic therapy / Chemotherapy / Apoptosis / p53 |
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| Research Abstract |
Objects: We designed to elevate therapeutic efficacy of radiation by addition of glycerol on an oral squamous cell carcinoma cell line (Ca9-22) bearing mutation in a tumor suppressor gene, p53 (mp53).
Methods: To examine the effectiveness of glycerol in radiation-induced lethality for Ca9-22 cells, we performed colony formation assay. Apoptosis was analyzed with Hoechst 33342 staining, Western blotting and DNA ladder formation assay. Ca9-22 cells became radiosensitive when glycerol was added to culture medium before X-ray irradiation.
Results: In accordance with the cell survived rate, apoptosis (apoptotic body and DNA fragmentation) was induced by X-rays irradiation after glycerol treatment while there was little apoptosis induced by X-ray irradiation or glycerol addition alone. Bax protein was accumulated after combined treatment of X-ray irradiation plus glycerol, but not after X-ray irradiation.
Conclusions: These findings suggested that pre-treatment with glycerol might be effective in therapy against mp53 cancer cells.
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