| Co-Investigator(Kenkyū-buntansha) |
KAKUTA Shigeru The University of Tokyo, Institute of Medical Science, Research Associate, 医科学研究所, 助手 (80345032)
IWAKURA Yoichiro The University of Tokyo, Institute of Medical Science, Professor, 医科学研究所, 教授 (10089120)
|
|---|
| Research Abstract |
Almost all the available embryonic stem (ES) cell lines are derived from 129 mice and have been used to produce genetic altered mice, because they can be maintained easily. However, 129 strains are neither well-characterized nor often used for immunological, neurological and developmental research. In most cases, It must take two years to backcross to other inbred strains, which are appropriate for the analysis. Therefore, Inbred ES cell, lines or rapid backcross are useful and desirable.
In this study, we established two inbred ES cell lines, 'ST/c' and 'ST/b', and tried rapid backcross using in vitro fertilization. ST/c derived from BALB/c mice were confirmed that it had the ability of germ line transmission. Using ST/c cells, the gene targeted clones were isolated and could produce chimera mice. In addition, using 129-derived ES cell line (E14.1), we established knockout mice disrupted the same gene, and it could take only 1/3 period to backcross the inbred strain by in vitro fertilization compared with traditional methods.
Now, we are trying the characterizations and gene alterations using C57BL/6-derived ES cell lines, ST/b and Bruce4.
|
