Studies on the health effects of exercise

2004 Fiscal Year Final Research Report Summary

• Project/Area Number: 14370140
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Public health/Health science
• Research Institution: Kagoshima University
• Principal Investigator: TAKEUCHI Toru Kagoshima University, Graduate School of Medical and Dental Sciences, Professor, 大学院・医歯学総合研究科, 教授 (00188161)
• Co-Investigator(Kenkyū-buntansha): AOYAMA Kohji Kagoshima University, Graduate School of Medical and Dental Sciences, Assistant Professor, 大学院・医歯学総合研究科, 講師 (70117472) ; YAMANAKA Takao National Institute of Fitness and Sports in Kanoya, College of Physical Education, Professor, 体育学部, 教授 (30136866) ; XU Baohui Kagoshima University, Graduate School of Medical and Dental Sciences, Research Associate, 大学院・医歯学総合研究科, 助手 (00264408) ; KOMATSU Masaharu Kagoshima University, Graduate School of Medical and Dental Sciences, Research Associate, 大学院・医歯学総合研究科, 助手 (30325815)
• Project Period (FY): 2002 – 2004
• Keywords: Exercise / Reactive Oxygen Species / Oxidative DNA damage / Anaerobic exercise / Aerobic exercies / Swimming / Fe-nitrilotriacetic acid / rat

Research Abstract

In this study we did the following experiments.
We have established a simple method to collect white blood cells from blood using dextran sulfate for the determination of 8-hydroxydeoxyguanosine(8OHdG), one of typical oxidative DNA damage. We have successfully applied this method for epidemiological studies.
We studied the difference in effects on oxidative damage between aerobic and anaerobic exercise. Postgraduate students were subjected to anaerobic or aerobic exercise using cycle ergometer. The exercise loads were the same in both exercise groups. Anaerobic exercise increased 8OHdG in white blood cells 24 hours after the exercise. Anaerobic exercise increased serum uric acid, suggesting the generation of superoxide by anaerobic exercise. Aerobic exercise did not increased 8OHdG or uric acid.
We studied the effects of habitual exercise on oxidative DNA damage and antioxidant enzymes. Rats were divided into two groups, one was sedentary group and the other was swimming group. Swimming group rats were subjected to swim, i.e. 15 to 60 min per swim, 5 times per week, for 10 weeks. Swimming did not increase 8OHdG in kidney. We injected Fe-nitrilotriacetic acid(Fe-NTA) into both groups to induce oxidative stress in kidney. Fe-NTA increased 8OHdG in kidney of both groups, however, Fe-NTA injection induced less 8OHdG in swimming group than in sedentary group. Swimming increased superoxide dismutase activity in kidney and diaphragm, but it did not increase catalase or glutathione peroxidase activities. Swimming decreased OGG1 protein levels in kidney. The data indicate that habitual exercise increased the resistance against oxidative stress and could be beneficial for cancer prevention.
We are trying to establish a method to determine 8OHdG repair activity by electrochemical detection after HPLC separation. We are using a synthetic oligonucleotides containing 8OHdG as a substrate. OGG1 activities as low as 0.01 units could be detected by the method.

Research Products

• [Journal Article] Habitual exercise induced resistance to oxidative stress. (2005)
  • Author(s): Nakatani K., Komatsu M., Kato T., Yamanaka T., Takekura H., Wagatsuma A., Aoyama K., Xu BH., Hirano T., Kasai H., Ando S., Takeuchi T.
  • Journal Title: Free Radical Research (in press)
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Generation of 8-hydroxydeoxyguanosine from DNA with Rat Lver Homogenates. (2005)
  • Author(s): Shi M., Takeshita H., Komatsu M., Xu BH., Aoyama K., Takeuchi T.
  • Journal Title: Cancer Science 96 Pages: 13-18
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Radical species in DNA strand-cleavage caused by dihydro-pyrazines. (2005)
  • Author(s): Kashige N., Takeuchi T., Matsumoto S., Takechi S., Miake F., Yamaguchi T.
  • Journal Title: Biol.Pharm.Bull. 28 Pages: 419-423
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Dual role of vitamin C in an oxygen-sensitive system discrepancy between DNA damage and cell death. (2005)
  • Author(s): Shi M., Xu BH., Azakami k., Morikawa t., Watanabe k., Morimoto k., Komatsu M., Aoyama K., Takeuchi T.
  • Journal Title: Free Radical Research (in press)
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Cisplatin resistance conferred by copper transporter, ATP7B. (2005)
  • Author(s): Komatsu, M., Furukawa, T., Nakagawa, M., Aoyama, K., Takeuchi, T., Akiyama, S.
  • Journal Title: Current Topics in Biotechnology (in press)
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Habitual exercise induced resistance to oxidative stress. (2005)
  • Author(s): Nakatani, K., Komatsu, M., Kato, T., Yamanaka, T., Takekura, H., Wagatsuma, A., Aoyama, K., Xu, BH., Hirano, T., Kasai, H., Ando, S., Takeuchi.T.
  • Journal Title: Free Radical Research (in press)
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Oxidative damages in chronic inflammation of a mouse autoimmune disease model. (2004)
  • Author(s): Shi M., Xu BH., Wang X., Aoyama K., Michie SA., Takeuchi T.
  • Journal Title: Immunology Letters 95 Pages: 233-236
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Academic influence on Japan of Edmund Alexander Parkes, pioneer of modern hygiene. (2004)
  • Author(s): Matsushita, T., Nomura, S., Takeuchi, T.
  • Journal Title: Environ.Health Prev.Med. 9 Pages: 9-12
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Method to overcome photoreaction, a serious drawback to the use of dichlorofluorescin in evaluation of ROS. (2003)
  • Author(s): Afzal, M., Matsugo, S., Sasai, M., Xu, BH., Aoyama, K., Takeuchi, T.
  • Journal Title: Biochem.Biophys.Res.Commun. 304 Pages: 619-624
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Role of Fas/Fas ligand-mediated apoptosis in murine contact hypersensitivity. (2003)
  • Author(s): Xu BH, Bulfone-Paus S, Aoyama K, Yu S, Huang PX, Morimoto K, Matsushita T, Takeuchi T.
  • Journal Title: Int.Immunopharmacol. 3 Pages: 927-938
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Lymphocyte homing to bronchus-associated lymphoid tissue(BALT) is mediated by L-selectin/PNAd, alpha4beta1 integrin/VCAM-1, and LFA-1 adhesion pathways. (2003)
  • Author(s): Xu B, Wagner N, Pham LN, Magno V, Shan Z, Butcher EC, Michie SA.
  • Journal Title: J Exp Med. 197 Pages: 1255-1267
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Helicobacter pylori-induced enlarged-fold gastritis is associated with increased mutagenicity of gastric juice, increased oxidative DNA damage, and an increased risk of gastric carcinoma. (2003)
  • Author(s): Nishibayashi H, Kanayama S, Kiyohara T, Yamamoto K, Miyazaki Y, Yasunaga Y, Shinomura Y, Takeshita T, Takeuchi T, Morimoto K, Matsuzawa Y.
  • Journal Title: J.Gastroenterol.Hepatol. 18 Pages: 1384-1391
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Lack of direct involvement of 8-hydroxy-2'-deoxyguanosine in hypoxanthine-guanine phophoribosyltransferase mutagenesis in V79 cells treated with N,N'-bis(2-hydroxy-peroxy-2-methoxyethyl) -1,4,5,8-naphthalenetetracarboxylic-diimide(NP-III) or riboflavin. (2002)
  • Author(s): Nakjima, M., Takeuchi, T., Ogino, K., Morimoto, K.
  • Journal Title: Jpn.J.Cancer Res. 92 Pages: 247-252
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Nitration and chlorination of folic acid by peroxynitrite and hypochlorous acid, and the selective binding of 1O-nitro-folate to folate receptor β (2002)
  • Author(s): Nakamura, M., Nagayoshi, R., Ijiri, K., Nakashima-Matsushita, N., Takeuchi, T., Matsuyama, T.
  • Journal Title: Biochem.Biophys.Res.Commun. 297 Pages: 1238-1244
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Lack of direct involvement of 8-hydroxy-2'-deoxyguanosine in hypoxanthine-guanine phophoribosyltransferase mutagenesis in V79 cells treated with N,N'-bis(2-hydroxyperoxy-2-methoxyethyl)-1,4,5,8-naphthalenetetracarboxylic-diimide(NP-III) or riboflavin. (2002)
  • Author(s): Nakjima, M., Takeuchi, T., Ogino, K., Morimoto, K.
  • Journal Title: Jpn.J.Cancer Res. 92 Pages: 247-252
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Expression of cytokine mRNA in mice cutaneously exposed to folmaldehyde. (2002)
  • Author(s): Xu, B., Aoyama, K., Takeuchi, M., Matsushita, T., Takeuchi, T.
  • Journal Title: Immunology Letters 84 Pages: 49-55
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Effect of hot water extract from Agaricus blazei Murill on antibody-producing cells in mice. (2002)
  • Author(s): Nakajima, A, Ishida, T., Koga, M., Takeuchi, T., Mazda, O., Takeuchi, M.
  • Journal Title: Int.Immunopharmacol. 2 Pages: 1205-1211
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Nitration and chlorination of folic acid by peroxynitrite and hypochlorous acid, and the selective binding of 10-nitro-folate to folate receptor β. (2002)
  • Author(s): Nakamura, M., Nagayoshi, R., Ijiri, K., Nakashima-Matsushita, N., Takeuchi, T., Matsuyama, T.
  • Journal Title: Biochem.Biophys.Res.Commun. 297 Pages: 1238-1244
  • Description: 「研究成果報告書概要(欧文)」より
• [Book] 環境保健(岸, 古野, 小泉, 大前編):New 予防医学・公衆衛生学(分担執筆) (2003)
  • Author(s): 竹内亨
  • Total Pages: 374
  • Publisher: 南江堂,京都
  • Description: 「研究成果報告書概要(和文)」より