2003 Fiscal Year Final Research Report Summary
The pathogenesis of Type 1 diabetes in children-the role of endogenous retrovirus infection in relation with disposition, environment and auto-immunity
| Project/Area Number |
14370251
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pediatrics
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| Research Institution | KITASATO UNIVERSITY |
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Principal Investigator |
MATSUURA Nobuo Kitasato Univ., School of Medicine, Professor, 医学部, 教授 (50002332)
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| Co-Investigator(Kenkyū-buntansha) |
JINNO Yoshihiro Ryukyu University School of Medicine, Department of Molecular Biology, Professor, 医学部・附属沖縄アジア医学研究センター・分子生物研究分野, 教授 (20179097)
KAZAHARI Kouji Kitasato Univ., School of Medicine, Research Assistant, 医学部, 助手 (90214288)
YOKOTA Yukifum Kitasato Univ., School of Medicine, Associate Professor, 医学部, 講師 (40158366)
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| Project Period (FY) |
2002 – 2003
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| Keywords | Type 1 diabetes mellitus / Susceptibility gene(s) / HLA-DQ gene / HLA-DR gene / SPIDDM / Entero-virus mRNA / IDDMK1,2-22 gene / Super-antigen |
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| Research Abstract |
We have studied the following projects during these two years 1) Isolation of Enterovirus m RNA from newly diagnosis of ketosis-phone type 1 diabetic children : 95 serum and 26 mononuclear cells samples from newly diagnosis type 1 diabetes were tried to extract entero-virus mRNA(VP4), however non of the serum samples nor mononuclear cells were failed to isolate virus mRNA. 2) We identified two nonsynonymous A/G polymorphisms in the superantigen-coding region of IDDMK_<1.2>22 at the 290-and 461-nucleotide position from the initial methione coden. Dr.Conrad B was interested in relation to super -antigen activity and this polymorphism and asked us to collaborate with type 1 diabetic children. However, in some reason, these collaborate studies were finally not succeeded due to their reasons. 3) Clinical and genetic characteristic of slowly progressing type 1 diabetes(SPIDDM) in children : We have analyzed HLA DQ-β, -αgene, DR-βgene, in children with acute-onset type 1 diabetes, SPIDDM, NIDDM and normal controls to identified the pathogenesis of SPIDDM. We found that age at onset of SPIDDM is much elder than acute onset and positivity of islet antibodies was almost same. HLA genes were different from NIDDM and controls. DRB1 0405-DQB1 0401 is popular and will be a subtype of type 1 diabetes mellitus.
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