2004 Fiscal Year Final Research Report Summary
Analysis and detection of the molecules involving hormone refractory prostate cancer by applying Proteomics methodology
| Project/Area Number |
14370517
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Urology
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| Research Institution | Yokohama City University |
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Principal Investigator |
KUBOTA Yoshinobu Yokohama City Univ., Dept. Urology, Professor, 大学院・医学研究科, 教授 (10106312)
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| Co-Investigator(Kenkyū-buntansha) |
UEMURA Hiroji Yokohama City Univ., Dept. Urology, Associate professor, 附属病院, 助教授 (50244439)
YAO Masahiro Yokohama City Univ., Dept. Urology, Associate professor, 大学院・医学研究科, 助教授 (00260787)
HIRANO Hisashi Yokohama City Univ., Kihara institute for biological research, Professor, 木原生物学研究所, 教授 (00275075)
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| Project Period (FY) |
2002 – 2004
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| Keywords | prostate cancer / hormone refractory cancer / proteomics / angiotensin II receptor / androgen |
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| Research Abstract |
To analyze the key molecules involving progression of prostate cancer to hormone refractory cancer, we have applied proteomics and other molecular biological methodologies in this research project.
We have identified several molecules which expressed diffentially between androgen dependent prostate cancer cells and androgen independent ones in 2D gel analysis by applying anti-body to tyrosin-kinase. We, then, have focused on the Angiotensin II receptor (AT1) molecules among them.
We demonstrated that AT1 receptar is expressed in human prostate cells. Especially, high expression level of AT1 receptor is seen in androgen independent prostate cancer cells. And stimulation by AII induces activation of intracellular signal transduction pathways that lead to the growth of prostate cancer cells. And we showed that candesartan, an angiatensin II receptor (AT1) blocker, inhibits both the growth of prostate cancer calls in vitro and that of tumors produced by prostate cancer cells in nude mice in vivo (Mol cancer Therap. 2003). We also suggested the potential of AT1 receptor blocker as a novel therapy for prostate cancer (J. Urol. 2005).
We think that these study results have made a remarkable contribution to elucidate the mechanisms of hormone refractory prostate cancer as well as to develop a new novel therapy for this disease.
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Research Products
(16 results)
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[Journal Article] Genistein induces cell growth inhibition in prostate cancer through the suppression of telomerase activity2005
Author(s)
Ouchi, H., Ishiguro, H., Ikeda, N., Honi, M., Kubota, Y., Uemura, H.
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Journal Title
International Journal of Urology 12
Pages: 73-80
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Effectiveness of percent free prostate specific antigen as a predictor of prostate cancer detection on repeat biopsy2004
Author(s)
Uemura, H., Nakamura, M., Hasumi, H., Sugiura, S., Fujinami, K., Miyoshi, Y., Yao, M., Kubota, Y.
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Journal Title
International Journal of Urology 11(7)
Pages: 494-500
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Combination Treatment with 1α, 25-Dihydroxyvitamin U3 and 9-cis-Retinoic Acid Directly Inhibits Human Telomerase Reverse Transcriptase Transcription in Prostate Cancer Cells2003
Author(s)
Ikeda, N., Uemura, H., Ishiguro, H., Hori, M, Hosaka, M., Kyo, S., Miyamoto, H., Takeda, E., Kubota, Y.
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Journal Title
Mol. Cancer Therap. 2
Pages: 739-746
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] 55 kDa nuclear matrix (nmt55) mRNA is expressed in human prostate cancer and is associated with androgen receptor2003
Author(s)
Ishiguro, H., Uemura, H., Fujinami, K., Ikeda, N., Ohta, S., Kubota, Y.
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Journal Title
Int. J. Cancer 105(1)
Pages: 26-32
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Expression of AR associated protein 55 (ARA55) and androgen receptor in prostate cancer2003
Author(s)
Miyoshi, Y., Ishiguro, H., Uemura, H., Fujinami, K., Miyamoto, H., Miyoshi, Y., Kitamura, H., Kubota, Y.
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Journal Title
The Prostate 56
Pages: 280-286
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Angiotensin II Receptor Blocker Shows Antiproliferative Activity in Prostate Cancer Cells: A Possibility of Tyrosine Kinase Inhibitor of Growth Factor2003
Author(s)
Uemura, H., Ishiguro, H., Nakaigawa, N., Nagashima, Y., Miyoshi, Y., Fujnami, K., Sakaguchi, A., Kubota, Y.
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Journal Title
Mol. Cancer Therap. 2
Pages: 1139-1147
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Association of common missense changes in ELAC2 (HPC2) with prostate cancer in a Japanese case-control series2002
Author(s)
Fujiwara, H., Emi, M., Nagai, H., Nishimura, T., Konishi, N., Kubota, Y., Ichikawa, T., Takahashi, S., Shuin, T., Habuchi, T., Qgawa, C., Inoue, K., Skolnick, H.M., Swensen, J., Camp, J.N., Tavtigian, V.S.
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Journal Title
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Inhibition of Growth of Human Prostate Cancer Xenograft by Transfection of p53 gene: Gene Transfer by Electroporation2002
Author(s)
Mikata, K., Uemura, H., Ohuchi, H., Ohta, S., Nagashima, Y., Kubota, Y.
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Journal Title
Mol. Cancer Therap. 1
Pages: 247-252
Description
「研究成果報告書概要(欧文)」より
