2005 Fiscal Year Final Research Report Summary
| Project/Area Number |
14390051
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
広領域
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| Research Institution | Kanagawa University |
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Principal Investigator |
INOUE Kazuhito KANAGAWA UNIVERSITY, FACULTY OF SCIENCE, PROFESSOR, 理学部, 教授 (20221088)
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| Co-Investigator(Kenkyū-buntansha) |
FUJITA Yuichi NAGOYA UNIVERSITY, GRADUATE SCHOOL OF BIOAGRICULTURAL SCIENCES, ASSOCIATE PROFESSOR, 大学院生命農学研究科, 助教授 (80222264)
NAGASHIMA Kenji V.P. TOKYO METROPOLITAN UNIVERSITY, DEPARTMENT OF BIOLOGY, RESEARCH ASSOCIATE, 都市教養学部理工学系, 助手 (80264589)
SUZUKI Yoshihiro KANAGAWA UNIVERSITY, FACULTY OF SCIENCE, ASSOCIATE PROFESSOR, 理学部, 助教授 (50301586)
KOBAYASHI Masami TSUKUBA UNIVERSITY, INSTITUTE OF MATERIALS SCIENCE, ASSOCIATE PROFESSOR, 物質工学系, 助教授 (70234846)
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| Project Period (FY) |
2002 – 2005
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| Keywords | PHOTOSYNTHESIS / PHOTOSYNTHETIC BACTERIA / PIGMENT / EVOLUTION / CHLOROPHYLL / CYANOBACTERIA / ENERGY METABOLISM / ENVIROMENTAL ADAPTATION |
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| Research Abstract |
The origin and evolution of photosynthesis have long remained enigmatic. To probe early evolutionary history of photosynthesis, we have investigated (B)Chl synthesis, biogenesis of photosynthetic apparatus, and electron transport of purple bacteria, green sulfur bacteria, heliobacteria and cyanobacteria. The summary of this study are as follows (Only the main results are shown).
(1)Chlorophyll synthesis : A number of genes involved in (B)Chl synthesis were isolated from various phototrophic prokaryote. Homologues of bchF and bchG from green-sulfur bacterium Chlorobium tepidum were introduced and expressed in non-sulfur bacterium Rhodobacter capsulatus and cyanobacteria Synechocystis sp.PCC6803. Novel pigments accumulated in the cells of R.capsulatus and Synechocystis.
(2)Biogenesis of photosynthetic apparatus : Assembly factors of Iron-sulfur cluster, IscR and IscS, from C.tepidum were overexpressed and characterized. Ferredoxin and iron-sulfur type RC were isolated from heliobacteria and they supported NADP^+ photoreduction in vitro. Nitrogenase-like enzyme, Dark POR, was isolated from R.capsulatus and characterized its biochemical properties.
(3)Electron transport : Bound cytochrome of Rubrivivax gelatinosus belongs tri-heme type, while that of Blastochloris viridis is tetra-heme type. Chimeric RC of R.gelatinosus which contained B.viridis cytochrome subunit was constructed and investigated their electron transport.
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Research Products
(76 results)
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[Journal Article] characterization of a nitrogenase-like enzyme catalyzing protochlorophyllideReduction from Rhodobacter capsulatus2005
Author(s)
Nomata, J., Inoue, K., Kitashima, M., Swen, L., Bauer, C., Fujita, Y.
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Journal Title
Photosynthesis : Fundamental Aspects to Global Perspectives(ed by A. var Est, D. Bruce)
Pages: 892-894
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Expression of 3-vinyl bacteriochlorophyllide hydratase bchF from purple non-sulfur bacterium Rhodobacter capsulatus and green sulfur bacterium...2005
Author(s)
Seki, T., Atsuta, M., Hashimoto, Y., Gotoh, T., Ito, Y., Kobayashi, M, Inoue,K.
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Journal Title
Photosynthesis : Fundamental Aspects to Global Perspectives(ed by A. var Est, D. Bruce)
Pages: 890-892
Description
「研究成果報告書概要(和文)」より
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[Journal Article] The reaction center associated triheme cytochrome of Rhodobacter sulfidophilum, structural analysis and evolutionary aspects.2005
Author(s)
Alric J., Tsukatani Y., Yoshida M., Matsuura K., Shimada K., Hienerwadel R., Schoepp-Cothenet B., Nitschke W., Nagashima K.V.P., Vermeglio A.
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Journal Title
Photosynthesis : Fundamental Aspects to Global Perspective(A.van der Est and D.Bruce eds.)(Allen Press Inc.Lawrenc, USA)
Pages: 285-287
Description
「研究成果報告書概要(欧文)」より
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