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2004 Fiscal Year Final Research Report Summary

Determination of genetic susceptibility factors for atmospheric toxic substances.

Research Project

Project/Area Number 14390058
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 広領域
Research InstitutionNational Institute for Environmental Studies

Principal Investigator

HIRANO Seishiro  National Institute for Environmental Studies, Environmental Health Sciences Division, Chief, 環境健康研究領域, 室長 (20150162)

Co-Investigator(Kenkyū-buntansha) CUI Xing  National Institute for Environmental Studies, Environmental Health Sciences Division, Researcher, 環境健康研究領域, 研究員 (20342735)
Project Period (FY) 2002 – 2004
KeywordsOxidative stress / Glutathione / Cytotoxicity / Heme oxigenase-1 / Urban particles / Arsenicals / RNA interference / Single nucleotide polymorphism
Research Abstract

We have addressed cytotoxicity and oxidative-stress potency of organic extracts of diesel exhaust particles (OE-DEP) and urban fine particles (OE-UFP) in rat heart microvessel endothelial (RHMVE) cells. mRNA levels of antioxidant enzymes including heme oxygenase-1 (HO-1) were increased following exposure to OE-DEP and OE-UFP as well as arsenicals. It has been reported that airborne arsenicals generate from coal combustion and cause chronic arsenicism in developing countries. We investigated effects of N-acetylcysteine, buthionine sulfoximine, and siRNA fro HO-1 on cytotoxicity of OE-DEP, OE-UFP, and arsenicals in endothelial cells. N-acetylcysteine did not affect the glutathione levels and reduce the cytotoxicity of arsenicals. Buthionine sulfoximine decreased cellular glutathione level and enhanced the cytotoxicity and expression of HO-1 mRNA. Transfection with siRNA for HO-1 decreased the HO-1 RNA level and enhanced the cytotoxicity of arsenicals except for dialkylarsenicals, suggesting that HO-1 plays a role in antioxidant effects. SNP analyzes were also performed to investigate polymorphism of acetaldehyde dehydrogenase 2.

  • Research Products

    (26 results)

All 2005 2004 2003

All Journal Article (25 results) Book (1 results)

  • [Journal Article] Accumulation and toxicity of monophenyl arsenicals in rat endothelial cells.2005

    • Author(s)
      S.Hirano, Y.Kobayashi, T.Hayakawa, X.Cui, M.Yamamoto, S.Kanno, A.Shraim
    • Journal Title

      Arch. Toxicol. 79

      Pages: 54-61

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] A new metabolic pathway of arsenite : Arsenic-glutathione complexes are substrates for human arsenic methyltransferase Cyt19.2005

    • Author(s)
      T.Hayakawa, Y.Kobayashi, X.Cui, S.Hirano
    • Journal Title

      Arch. Toxicol 79

      Pages: 183-191

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Stability of arsenic metabolites, arsenic triglutathine [As(GS)_3] and methylarsenic diglutathione [CH_3As(GS)_2], in rat bile.2005

    • Author(s)
      Y.Kobayashi, X.Cui, S.Hirano
    • Journal Title

      Toxicology 211

      Pages: 115-123

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Accumulation and toxicity of monophenyl arsenicals in rat endothelial cells.2005

    • Author(s)
      S.Hirano, Y.Kobayashi, T.Hayakawa, X.Cui, M.Yamamoto, S.Kanno, A.Shraim
    • Journal Title

      Arch.Toxicol. 79

      Pages: 54-61

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] A new metabolic pathway of arsenite : Arsenic-glutathione complexes are substrates for human arsenic methyltransferase Cyt19.2005

    • Author(s)
      T.Hayakawa, Y.Kobayashi, X.Cui, S.Hirano
    • Journal Title

      Arch.Toxicol. 79

      Pages: 183-191

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Stability of arsenic metabolites, arsenic triglutathione [As(GS)_3] and methylarsenic diglutathione [CH_3As(GS)_2], in rat bile.2005

    • Author(s)
      Y.Kobayashi, X.Cui, S.Hirano
    • Journal Title

      Toxicology 211

      Pages: 115-123

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Nanoparticles in Emissions and Atmospheric Environment-Now and Future-.2004

    • Author(s)
      S.Hirano, H.Nitta, Y.Moriguchi, S.Kobayashi, Y.Kondo, K.Tanabe, et al.
    • Journal Title

      J. Nanoparticles Res. 3

      Pages: 311-321

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Effects of phytoestrogens and environmental estrogens on osteblastic differentiation in MC3T3-E1 cells.2004

    • Author(s)
      S.Kannno, S.Hirano, F.Kayama
    • Journal Title

      Toxicology 196

      Pages: 137-145

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] The accumulation and toxicity of methylated arsenicals in endothelial cells : Important roles of thiol compounds.2004

    • Author(s)
      S.Hirano, Y.Kobayashi, X.Cui, S.Kanno, T.Hayakawa, A.Shraim
    • Journal Title

      Toxicol. Appl. Pharmacol. 198

      Pages: 458-467

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Effects of the phytoestrogen coumestrol on RANK-ligand-induced differentiation of osteoclasts.2004

    • Author(s)
      S.Kanno, S.Hirano, F.Kayama
    • Journal Title

      Toxicology 203

      Pages: 211-220

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Arsenic speciation in bile and urine following oral and intravenous exposure to inorganic and organic arsenics in rats.2004

    • Author(s)
      X.Cui, Y.Kobayashi, T.Hayakawa, S.Hirano
    • Journal Title

      Toxicol. Sci. 82

      Pages: 478-487

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Effects of phytoestrogens and environmental estrogens on osteoblastic in MC3T3-E1 cells.2004

    • Author(s)
      S.Kanno, S.Hirano, F.Kayama
    • Journal Title

      Toxicology 196

      Pages: 137-145

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] The accumulation and toxicity of methylated arsenicals in endothelial cells : Important roles of thiol compounds.2004

    • Author(s)
      S.Hirano, Y.Kobayashi, X.Cui, S.Kanno, T.Hayakawa, A.Shrim
    • Journal Title

      Toxicol.Appl.Pharmacol. 198

      Pages: 458-467

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Effects of the phytoestrogen coumestrol on RANK-ligand-induced2004

    • Author(s)
      S.Kanno, S.Hirano, F.Kayama
    • Journal Title

      Toxicology 203

      Pages: 211-220

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Arsenic speciation in bile and urine following oral and intravenous exposure to inorganic and organic arsenics in rats.2004

    • Author(s)
      X.Cui, Y.Kobayashi, T.Hayakawa, S.Hirano
    • Journal Title

      Toxicol Sci. 82

      Pages: 478-487

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] ヒ素2004

    • Author(s)
      平野靖史郎, 小林弥生
    • Journal Title

      日本臨床 -増刊-(日本臨床社)

      Pages: 522-525

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Arsenic speciation in the urine and hair of individuals exposed toair borne arsenic through coal-burning in Guizhou, PR China.2003

    • Author(s)
      A.Shraim, X.Cui, S.Li, J.Ng, J.Wang, Y.Jin, Y.Liu, L.Guo, D.Li, S.Wang, R.Zhang, S.Hirano
    • Journal Title

      Toxicol. Lett. 137

      Pages: 35-48

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] The Difference in Uptake and Toxicity of Trivalent and Pentavalent Inorganic Arsenics in Rat Heart Microvessel Endothelial Cells.2003

    • Author(s)
      S.Hirano, X.Cui, S.Li, S.Kanno, Y.Kobayashi, T.Hayakawa, A.Shraim
    • Journal Title

      Arch. Toxicol. 77

      Pages: 305-312

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Oxidative-Stress Potency of Organic Extracts of Diesel Exhaust and Urban Fine Particles in Rat Heart Microvessel Endothelial Cells2003

    • Author(s)
      S.hirano, A.Furuyama, E.Koike, T.Kobayashi
    • Journal Title

      Toxicology 187

      Pages: 161-170

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] PCR-based Subtraction Analyses for Upregulated Gene Transcription in Cadmium-exposed Rat Lung Type 2 Epithelial Cells.2003

    • Author(s)
      S.Hirano, H.Kitajima, T.Hayakawa, X.Cui, S.Kanno, Y.Kobayashi, M.Yamamoto
    • Journal Title

      Biochem. Biophys. Res. Commun. 308

      Pages: 133-138

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Arsenic speciation in the urine and hair of individuals exposed to airborne arsenic through coal-burning in Guizhou, PR China.2003

    • Author(s)
      A.Shraim, X.Cui, S.Li, J.Ng, J.Wang, Y.Jin, Y.Liu, L.Guo, D.Li, S.Wang, R.Zhang, S.Hirano
    • Journal Title

      Toxicol.Lett. 137

      Pages: 35-48

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] The Difference in Uptake and Toxicity of Trivalent and Pentavalent Inorganic Arsenics in Rat Heart Micro vessel Endothelial Cells.Arch.2003

    • Author(s)
      S.Hirano, X.Cui, S.Li, S.Kanno, Y.Kobayashi, T.Hayakawa, A.Shraim
    • Journal Title

      Toxicol. 77

      Pages: 305-312

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Oxidative-Stress Potency of Organic Extracts of Diesel Exhaust and Urban Fine Particles in Rat Heart Microvessel Endothelial Cells.2003

    • Author(s)
      S.Hirano, A.Furuyama, E.Koike, T.Kobayashi
    • Journal Title

      Toxicolgy 187

      Pages: 161-170

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] PCR -based Subtraction Analyses for Upregulated Gene Transcription in Cadmium-exposed Rat Lung Type 2 Epithelial Cells.2003

    • Author(s)
      S.Hirano, H.Kitajima, T.Hayakawa, X.Cui, S.Kanno, Y.Kobayashi, M.Yamamoto
    • Journal Title

      Biochem.Biophys.Res.Commun. 308

      Pages: 133-138

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Nanoparticles in Emissions and Atmospheric Environment - Now and Future -.2003

    • Author(s)
      S.Hirano, H.Nitta, Y.Moriguchi, S.Kobayashi, Y.Kondo, K.Tanabe, T.Kobayashi, S.Wakamatsu, M.Morita, S.Yamazaki
    • Journal Title

      J.Nanoparticle Res. 3

      Pages: 311-321

    • Description
      「研究成果報告書概要(欧文)」より
  • [Book] ヒ素(In"日本臨床 -増刊-")2004

    • Author(s)
      平野靖史郎, 小林弥生
    • Total Pages
      4
    • Publisher
      日本臨床社
    • Description
      「研究成果報告書概要(和文)」より

URL: 

Published: 2006-07-11  

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