2003 Fiscal Year Final Research Report Summary
Treatment for chronic myocardial ischemia using autologous bone marrow derived mononuclear cells ; Therapeutic angiogenensis by myocardial injection catheter
Project/Area Number |
14570704
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Circulatory organs internal medicine
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Research Institution | KURUME UNIVERSITY |
Principal Investigator |
UENO Takafumi Kurume University, Dept of Int. Med III, associate professor, 医学部, 助教授 (90184952)
|
Co-Investigator(Kenkyū-buntansha) |
HONMA Tomoki Kurume University, Dept of Int. Med III, associate professor, 医学部, 助教授 (10181562)
IKEDA Hisao Kurume University, Dept of Int. Med III, associate professor, 医学部, 助教授 (50168134)
|
Project Period (FY) |
2002 – 2003
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Keywords | angiogenesis / bone marrow mononuclear cells / catheter-based injection / myocardial ischemia |
Research Abstract |
Purpose of this study is to test the hypothesis that transplantation of autologous bone marrow-derived mononuclear cells (BM-MNCs) into ischemic myocardium using a catheter-based approach might augment neovascularization and promote recovery of ventricular function. Animals were injected with either saline or BM-MNCs into the ischemic area using an intramyocardial injection catheter. BM-MNCs were isolated using a cell density gradient method. Two groups of pigs received intramyocardial injections of either BM-MNCs (n=5) or saline (n=5). Neither contrast angiography of collateral flow nor echocardiographic wall motion scores during dobutamine stress were different between the groups at baseline, 4 weeks post treatment, improvement in wall motion was not seen in the treatment group during peak stress. Myocardial blood flow in the ischemic region was significantly increased from baseline for pigs receiving BM-MNCs (0.31_0.65ml/min/g). The capillary density in the ischemic area was significantly higher in myocardial tissue from pigs treated with BM-MNCs than in tissue from pigs treated with saline. In conclusion, transplantation of autologous BM-MNCs using an intramyocardial injection catheter contributed to angiogenesis and improved cardiac function in chronically ischemic myocardium in pigs.
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[Publications] Ishii M, Ueno T, Ikeda H, lemura M, Sugimura T, Furui J, Sugahara Y, Muta H, Akagi T, Nomura Y, Honima T, Yokoi H, Nobuyoshi M, Matsuishi T, Kato H.: "Sequential follow-up results of catheter intervention for coronary artery lesions after Kawasaki disease : quantitative coronary artery angiography and intravascular ultrasound imaging study."Circulation. 105. 3004-3010 (2002)
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「研究成果報告書概要(欧文)」より
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[Publications] Katoh A, Ikeda H, Takajo Y, Haramaki N, Murohara T, Shintani S, Kanaya S, Yokoyama S, Ueno T, Homna T, Imaizumi T.: "Coexistence of impairment of endothelium-derived nitric oxide and platelet-derived nitric oxide in patients with coronary risk factors."Circ J. 66(9). 837-840 (2002)
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「研究成果報告書概要(欧文)」より
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