2004 Fiscal Year Final Research Report Summary
Clinical Significance of telomerase activation in gynecological malignancies
| Project/Area Number |
14571570
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Saga University, Faculty of Medicine (2004)
佐賀医科大学 (2002-2003) |
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Principal Investigator |
IWASAKA Tsuyoshi Saga Univ., Faculty of Medicine Ob/Gyn, Professor, 医学部, 教授 (60117067)
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| Co-Investigator(Kenkyū-buntansha) |
YOKOYAMA Masatoshi Saga Univ.Faculty of Medicine, Ob/Gyn, Associate Professor, 医学部, 助教授 (40230669)
NAKAO Yoshifumi Saga Univ.Faculty of Medicine, Ob/Gyn, Instructor, 医学部, 助手 (30336119)
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| Project Period (FY) |
2002 – 2004
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| Keywords | Cervical intraepithelial neoplasia / Cervical carcinoma / Ovarian malignancy / Telomerase / Radiotherapy / Epigallocatechin gallate / Apoptosis |
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| Research Abstract |
1)Measurement of telomerase activity as a screening method for cervical carcinoma : Cervical cytology and telomerase activity were examinined in 245 women as a mass screening. It was revealed that 16 of 244 cytology-negative women were positive for telomerase activation and 9 of 16 telomerase-positive women were also positive for high-risk HPV DNA and 9 of 11 telomerase-positive women of whom bipsied cervical specimens were available revealed CIN I lesion. The above results suggest that telomerase activation has a close relationship to high-risk HPV infection and cervical neoplasia, which means that measurement of telomerase activity is useful for cervical cancer screening.
2)Co-relation between telomerase positivity and outcome of CIN lesion : Sixty-one patients with CIN lesion (37 cases of CIN I and 24 cases of CIN II) were followed up for more than 5 years after evaluation of telomerase acivity. As a result, 8% and 50% of telomerase-positive patients with CIN I and CIN II, respectively, progressed to CIN III or more, while only 11% and 11% of telomerase-negative patients with CIN I and VIN II, respectively, progressed to CIN III. Telomerase-positive lesions have a tendency to progress to higher lesions, however, the difference was not significant.
3)Measurement of telomerase activity in abdominal fluid of patients with ovarian malignancy : It was revealed that telomerase activity was very low in abdominal fluid even in patients with disseminated disease, which suggests that telomerase assay cannot replace with the tool of washing cytology.
4)The effects of epigallocatechin gallate on the premalignant and malignant cervical cell lines : It is known that telomerase activation occurs during the process of cervical carcinogenesis and that epigallocatechin gallate(EG) is effective as an agent of chemoprevention for cervical carcinogenesis. It is revealed that EG induced apoptosis and inhibited telomerase activity in cervical premalignant and malignant cell lines.
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[Journal Article] IgG antibodies to human papillomavirus 16,52,58,and 6 L1 capsids : case-control study of cervical intraepithelial neoplasia in Japan.2003
Author(s)
Matsumoto K, Yoshikawa H, Yasugi T, nakagawa S, Kawana K, Takeoka A, Yaegashi N, Iwasaka T, Kanazawa K, Taketani Y, Kanda T.
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Journal Title
J Med Virol 69
Pages: 441-446
Description
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[Journal Article] Prognostic factors associated with the clinical outcome of cervical intraepithelial neoplasia: a cohort study in Japan.2003
Author(s)
Yokoyama M, Iwasaka T, Nagata C, Nozawa S, Sekiya S, Hirai Y, Kanazawa K, Sato S, Hoshiai H, Sugase M, Kawana T, Yoshikawa H.
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Journal Title
Cancer Letters 192
Pages: 171-179
Description
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[Journal Article] Ectopic chromosome around centrosome in metaphase cells as a marker of high-risk human papillomavirus-associated cervical intraepithelial neoplasias.2003
Author(s)
Furuta R, Hirai Y, Katase K, Tase S, Kawaguchi T, Akiyama F, Kato Y, Kumada K, Iwasaka T, Yaegashi N, Kanazawa K, Yoshikawa H, Kitagawa T.
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Journal Title
Int J Cancer 106
Pages: 167-171
Description
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[Journal Article] Characterization and imprinting status of OBPH1/Obph1 gene : Implications for an extended imprinting domain in human and mouse.2002
Author(s)
Higashimoto K, Soejima H, Yatsuki H, Joh K, Uchiyama M, Obata Y, Ono R, Wang Y, Xin Z, Zhu X, Masuko S, Ishino F, Hatada I, Jinno Y, Iwasaka T, Katsuki T Mukai T
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Journal Title
Genomics 80
Pages: 575-584
Description
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