2015 Fiscal Year Annual Research Report
合成微小環境を用いた軟骨組織のｉｎ ｖｉｔｒｏ構築
| Project/Area Number |
14F04106
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| Research Institution | Okayama University |
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Principal Investigator |
松本 卓也 岡山大学, 医歯(薬)学総合研究科, 教授 (40324793)
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| Co-Investigator(Kenkyū-buntansha) |
HARA EMILIO 岡山大学, 医歯(薬)学総合研究科, 外国人特別研究員
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| Project Period (FY) |
2014-04-25 – 2016-03-31
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| Keywords | 軟骨 / 合成微小環境 / DNAメチル化 / 石灰化 |
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| Outline of Annual Research Achievements |
The purpose of this study was to identify new methods and culture conditions to enhance chondrogenic differentiation of human bone marrow-derived mesenchymal stem/progenitor cells (hBMSCs) by applying chemical, physical and biological cues, including overexpression of DNA methyltransferases (DNMTs) in cells. We attempted to fabricate a chemically- and physically-controlled microenvironment using materials (e.g., hydrogels) that recapitulate native cell niche characteristics to optimally modulate the chondrogenic differentiation of BMSCs. Besides the physical and chemical stimulations, we also evaluated the chondrogenic differentiation of hBMSCs after overexpression of DNMTs that eventually induce DNA methylation at CPG rich regions of key genes. To study the effect of DNMTs on chondrogenesis of hBMSCs, we transfected overexpression vectors of DNMT3A, DNMT3B and the pcDNA control in hBMSCs, and culture the cells in a 3D micromass culture system with growth factors and glucocorticoids.
Additionally, since in vitro synthesized cartilage tissue generally undergoes through mineralization, we also attempted to understand the mechanisms involved in the chondrocyte death associated with initial mineralization process by using the secondary ossification center of mouse femur as in vivo model.
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| Research Progress Status |
27年度が最終年度であるため、記入しない。
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| Strategy for Future Research Activity |
27年度が最終年度であるため、記入しない。
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[Presentation] A high-throughput screening identified fluocinolone acetonide as a potent synergistic factor of TGF-β3-mediated chondrogenesis of BMSCs for articular surface repair.2015
Author(s)
Hara ES, Ono M, Pham HT, Sonoyama W, Kubota S, Takigawa M, Young MF, Olsen BR, Matsumoto T, Kuboki T.
Organizer
American Society for Bone and Mineral Research Annual Meeting
Place of Presentation
シアトル, USA
Year and Date
2015-10-12 – 2015-10-12
Int'l Joint Research
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[Presentation] A Novel Method to Induce Strong Chondrogenesis of BMSCs and Promote Articular Surface Repair using Fluocinolone Acetonide and TGF-β3.2015
Author(s)
Hara ES, Ono M, Pham HT, Sonoyama W, Kubota S, Takigawa M, Young MF, Olsen BR, Matsumoto T, Kuboki T.
Organizer
Biomedical Engineering Society Annual Meeting
Place of Presentation
タンパ, USA
Year and Date
2015-10-08 – 2015-10-08
Int'l Joint Research
