2005 Fiscal Year Final Research Report Summary
De4veropment of rRNA database for yeast type strains and multiple gene analysis for yeast taxonomy
| Project/Area Number |
15380062
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied microbiology
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| Research Institution | Osaka University |
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Principal Investigator |
SEKI Tatsuji Osaka University, ICBiotech, Professor, 物理工学国際交流センター, 教授 (50029245)
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| Co-Investigator(Kenkyū-buntansha) |
NAKAGAWA Hiroko (KAWASAKI Hiroko) Osaka University, ICBiotech, Assistant, 物理工学国際交流センター, 助手 (30251482)
FUJIYAMA Kazuhito Osaka University, ICBiotech, Assoc.Professor, 物理工学国際交流センター, 助教授 (70209112)
SUGITA Takashi Meigifermasutical Univerisity, Associate Professor, 微生物学教室, 助教授 (10312076)
HAMAMOTO Makiko Meigi University, Assoc.Professor, 農学部, 助教授 (30333305)
SUZUKI Motofumi Bioresourse center RIKEN, Senior Researcher, バイオリソースセンター, 先任研究員 (70163011)
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| Project Period (FY) |
2003 – 2005
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| Keywords | rRNA / rDNA / taxonomy / phylogeny / ITS / yeast / classification / database |
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| Research Abstract |
In this research, production of the rRNA database of yeasts and evaluation as a classification index of rRNA gene information were performed. Furthermore, molecular systematic analysis using a multiplex gene is conducted, and it tried to find out the new method and new concept for developing the classification f yeasts.
In many classification groups, those molecular information and analysis could be conducted simultaneously with discovery of a new microbe, and the rRNA gene information of many types of yeast was able to be collected. The open database of yeast molecular taxonomy was started to set up.
Based on the multiple gene analysis, it was founded that the substitution rate of the 18S rDNA is late compared with the substitution rate of the 26S rRNA D 1/D2 domain in all of the analyzed genera. There are a position which is rich in mutation, and a position which is not so in rRNA gene. It seemed that the parts of the rRNA gene could be properly used as identification in a genus level and species level. Interestingly the continuity in the homology of 26S rDNA D1/D2 domain within the same genus was shown, but the discontinuity of 18S rRNA gene were found. Especially the species of genus Pichia were devided to several phylogenetic groups.
We could summarize the molecular systematic of yeasts and proposed the new concepts of yeast classification.
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[Journal Article] DNA sequence diversity of intergenic spacer 1 region in the non-lipid-dependent species Malassezia pachydermatis isolated from animals.2005
Author(s)
T.Sugita, K.Takeo, K.Hama, E.Virtudazo, M.Takashima, A.Nishikawa, J.Kucsera, J.Dorogi, S.Komori, K.Nakagaki, A.Vollekova, E.Slavikova, V.Farkas.
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Journal Title
Med.Mycol. 43
Pages: 21-26
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Three new thermotolerant methylotrophic yeasts, Candida krabiensis sp. nov., Candida sithepensis sp. nov., and Pichia siamensis sp. nov., isolated in Thailand.2004
Author(s)
S.Limtong, N.Srisuk, W.Yongmanitchai, H.Kawasaki, H.Yurimoto, T.Nakase, N.Kato.
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Journal Title
J.Gen.Appl.Microbiol. 50
Pages: 119-127
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Candida easanensis sp. nov., Candida pattaniensis sp. nov. and Candida nakhonratchasimensis sp. nov., three new species of yeasts isolated from insect frass in Thailand.2004
Author(s)
S.Jindamorakot, S.Am-in, T.T.Tran, D.D.Ngo, H.Kawasaki, W.Potacharoen, S.Limtong, M.Tanticharoen, T.Nakase.
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Journal Title
J.Gen.Appl.Microbiol. 50
Pages: 261-269
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Fluconazole-resistant pathogens Candida inconspicua and C. norvegensis : DNA sequence diversity of the rRNA intergenic spacer region, antifungal drug susceptibility, and extracellular enzyme production.2004
Author(s)
T.Sugita, K.Takeo, M.Ohkusu, E.Virtudazo, M.Takashima, E.Asako, F.Ohshima, S.Harada, C.Yanaka, A.Nishikawa, L.Majoros, M.Sipiczki.
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Journal Title
Microbiol. Immunol. 48
Pages: 761-766
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] The first isolation of ustilaginomycetous anamorphic yeasts, Pseudozyma species, from patients blood and a description of two new species : P. parantarctica and P. thailandica2003
Author(s)
T.Sugita, M.Takashima, N.Poonwan, N.Mekha, K.Malaithao, B., Thungmuthasawat, S.Prasarn, P.Luangsook, T.Kudo.
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Journal Title
Microbiol.Immunol 47
Pages: 183-190
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Sporobolomyces magnisporus sp. nov., a new yeast species in the Erythrobasidium cluster isolated from plants in Taiwan.2003
Author(s)
T.Nakase, S.Tsuzuki, FL.Lee, T.Sugita, S.Jindamorakot, H.Jan-ngam, W.Potacharoen, M.Tanticharoen, T.Kudo, M.Takashima.
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Journal Title
J.Gen.Appl.Microbiol. 49
Pages: 337-344
Description
「研究成果報告書概要(欧文)」より
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