2005 Fiscal Year Final Research Report Summary
Clarification of survival strategy and host recognition mechanism of root parasitic weeds
| Project/Area Number |
15380079
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Bioproduction chemistry/Bioorganic chemistry
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| Research Institution | Utsunomiya University |
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Principal Investigator |
YONEYAMA Koichi Utsunomiya University, Center for Research on Wild Plants, Professor, 野生植物科学研究センター, 教授 (00114174)
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| Co-Investigator(Kenkyū-buntansha) |
TAKEUCHI Yasutomo Utsunomiya University, Center for Research on Wild Plants, Professor, 野生植物科学研究センター, 教授 (90008003)
NATSUAKI Tomohide Utsunomiya University, Faculty of Agriculture, Professor, 農学部, 教授 (10134264)
SEKIMOTO Hitoshi Utsunomiya University, Faculty of Agriculture, Professor, 農学部, 教授 (10261819)
YOKOTA Takao Teikyo University, Faculty of Science and Technology, 理工学部, 教授 (40011986)
KUWAHARA Shigefumi Tohoku University, Graduate School of Agriculture, 大学院・農学研究科, 教授 (30170145)
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| Project Period (FY) |
2003 – 2005
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| Keywords | root parasitic weeds / germination stimulants / strigolactone / LC / MS / MS / host recognition mechanism / carotenoid biosynthesis / P450 monooxygenase / ABC transporter |
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| Research Abstract |
Characterization of strigolactones, germination stimulants for root parasitic weeds, Striga and Orobanche, exuded by their host plants was conducted. One of the major strigolactones of monocotyledonous plants, maize, millet and sorghum, was found to be deoxystrigol which is known as a host recognition signal for arbuscular mycorrhizal (AM) fungi. On the other hand, one of the major strigolactones of dicotyledonous plants was identified as orobanchol. In addition to these known strigolactones, at least 10 novel strigolactones were found in plant species examined. Non-mycorrhizal plants, such as spinach and white lupin, and Japanese red pine tree were found to exude strigolactones. These results indicate that strigolactones are distributed widely in the plant kingdom and other factors as well as strigolactones appear to be involved in host recognition of AM fungi.
Through LC/MS/MS analyses of orobanchol in the root exudates, it was first demonstrated that in red clover plants reduced supply of phosphorus but not of other mineral nutrients examined (N, K, Mg, Ca) in the culture medium significantly promoted the release of orobanchol by the root of the plant. In addition, 10 nM fluridone, an inhibitor of carotenoid biosynthesis, reduced orobanchol exudation markedly, supporting the hypothesis that strigolactones are synthesized via carotenoids in planta. This carotenoid biosynthetic inhibitor alone elicited seed germination of Striga asiatica and also induced haustorium-like morphogenesis in the germinated seeds.
DNA microarray analyses of Arabidopsis thaliana treated with strigol suggested that several genes including two P450 genes and ABC transporter gene are potentially involved in the biosynthesis and metabolism of strigolactones.
Further study on biosynthesis and metabolism of strigolactones should shed light to both symbiotic and parasitic interactions in the rhizosphere.
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Research Products
(16 results)
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[Journal Article] Effects of fluridone and norflurazon on conditioning and germination of Striga asiatica seeds.2006
Author(s)
Kusumoto, D., Chae, S.H., Mukaida, K., Yoneyama, K., Yoneyama, K., Joel, D.M., Takeuchi, Y.
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Journal Title
Plant Growth Regulation 48
Pages: 73-78
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Characterization of strigolactones, germination stimulants for the root parasitic plants Striga and Orobanche, produced by maize, millet and sorghum.2006
Author(s)
Awad, A.A., Sato, D., Kusumoto, D., Kamioka, H., Takeuchi, Y., Yoneyama, K.
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Journal Title
Plant Growth Regulation 48
Pages: 221-227
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Confirmation and quantification of strigolactones, germination stimulants for root parasitic plants Striga and Orobanche, produced by cotton.2005
Author(s)
Sato, D., Awad, A.A., Takeuchi, Y., Yoneyama, K.
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Journal Title
Bioscience, Biotechnology, and Biochemistry 69(1)
Pages: 98-102
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Interaction between Orobanche crenate and its host legumes : unsuccessful haustorial penetration and necrosis of the developing parasite.2005
Author(s)
Perez-de-Luque, A., Rubiales, D., Cubero, J.I., Press, M.C., Scholes, J., Yoneyama, K., Takeuchi, Y., Plakhine, D., Joel, D.M.
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Journal Title
Annals of Botany 95
Pages: 935-942
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Germination response of Orobanche seeds subjected to conditioning temperature, water potential and growth regulator treatments.2005
Author(s)
Song, W.J., Zhou, W.J., Jin, Z.L., Cao, D.D., Joel, D.M., Takeuchi, Y., Yoneyama, K.
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Journal Title
Weed Research 45
Pages: 467-476
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] In vitro infection of host roots by differentiated calli of the parasitic plant Orobanche2004
Author(s)
Zhou, W.J., Yoneyama, K., Takeuchi, Y., Iso, S., Rungmekarat, S., Chae, S.H., Sato, D., Joel, D.M.
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Journal Title
Journal of Experimental Botany 55
Pages: 899-907
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Search for germination stimulants and inhibitors for root parasitic weeds.
Author(s)
Yoneyama, K., Sate, D., Takeuchi, Y, Sekimoto, H., Yokota, T., Sassa, T.
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Journal Title
American Chemical Society Symposium Series 927
Pages: 88-98
Description
「研究成果報告書概要(欧文)」より
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