2006 Fiscal Year Final Research Report Summary
Some enzymes in the cerebrospinal fluid and glyco-conjugates may be useful to diagnose brain disorder
| Project/Area Number |
15390212
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Legal medicine
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| Research Institution | Shiga University of Medical Science |
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Principal Investigator |
NISHI Katsuji Shiga University of Medical Science, Medical, professor, 医学部, 教授 (60073681)
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| Co-Investigator(Kenkyū-buntansha) |
TAKATSU Akihiro The Jikei University School of Medicine, Medical, professor, 医学部, 教授 (60010089)
OHKUBO Iwao Shiga University of Medical Science, Medical, professor, 医学部, 教授 (80152073)
YAMAMOTO Yoshio Shiga University of Medical Science, Medical, Assistant Professor, 医学部, 助手 (60111902)
IKEMOTO Keiko Fukushima Medical University, Medical, Lecturer, 医学部, 講師 (90184449)
TAKASE Izumi Shiga University of Medical Science, Medical, Assistant Professor, 医学部, 助手 (30351406)
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| Project Period (FY) |
2003 – 2006
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| Keywords | Protease and Protease Inhibitor / CSF / Glycoconjugate / Brain Disorder / シスタチンC / プロスタグランヂンD2 |
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| Research Abstract |
Some enzymes contained in the cerebrospinal fluid (CSF) were purified and antibodies against those enzymes were produced. Puromycin sensitive alanyl-amninopeptidase (PSA) increased its amount in the CSF with post-mortem interval and was detected in senile plaque of the brain with dementia. Cystatin C (CC) acting as a protease inhibitor was clearly detected in the old-infarction area of the brain. Prostaglandin D2 synthase (PGDS), beta-trace, was also detected in the brain bleeding area. These results indicate that PSA, CC and PGDS may be useful in the forensic neuropathology. PGDS known as sleeping control substance was detected in the neurons of cerebellum and medulla oblongata with COX 2, showing the PGDS may relate with posture-regulation during one's sleeping. A large amount of Le X antigen known as stage specific glycoconjugate antigen existed in the hippocampus and the cerebellum. The existence area of the Le X antigen was differ as one's grows, that is, on the neurons in the hippocampus from three-month old-baby, on the astrocytes in the hippocampus from one-year-old baby, and after one-year-old on the astrocytes and micro-glia cells. The carbohydrate depositions were detected in the molecular layer of the dentate gyrus of the hippocampus from patients with memorial disturbance.
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[Journal Article] Immuno-histochemical characteristics of necrotic area in the old-infarction area of human brain bu using of antibodies against cystatin c cathepsin B, puromycin sensitive alanyl-aminopeptidase and prostaglandin D2 synthase - A preliminary oxamination.2007
Author(s)
A.Kobayashi, I.Takase, T.Kitamura, A.Takamure, M.Ohyagi, A.Morimoto, S.Yamasaki, Y.Yamamoto, I.Ohkubo, K.Nishi, Anil Aggrawa
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Journal Title
Internet Journal of Forensic Medicine and Toxicology 8(In press)
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Legumain/asparaginyl endopeptidase controls extracellular matrix remodeling through the degradation of fibronectin in mouse renal proximal tubular cells.2006
Author(s)
Y.Morita, H.Araki, T.Sugimoto, K.Takeuchi, T.Yamane, T.Maeda, Y.Yamamoto, K.Nishi, M.Asano, K.Noda, M.Nishimura, T.Uzu, I.Nishimura, D.Koya, A.Kashiwagi, I.Ohkubo
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Journal Title
FEBS Letters 8
Pages: 1417-1424
Description
「研究成果報告書概要(和文)」より
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