2005 Fiscal Year Final Research Report Summary
Evaluation of cell damage and allergy-induced mechanism by minute substance eluted from dental bio-materials
| Project/Area Number |
15390597
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
補綴理工系歯学
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| Research Institution | Iwate Medical University |
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Principal Investigator |
TAIRA Masayuki Iwate Medical University School of Dentistry, Dept. of Dental Materials, Associate Professor, 歯学部, 助教授 (60179398)
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| Co-Investigator(Kenkyū-buntansha) |
ARAKI Yoshima Iwate Medical Univ. School of Dentistry, Dept. of Dental Materials, Professor, 歯学部, 教授 (20005036)
SAITOH Setsuo Iwate Medical Univ. School of Dentistry, Dept. of Dental Materials, Lecturer, 歯学部, 講師 (70137537)
SASAKI Minoru Iwate Medical Univ. School of Dentistry, Dept. of Oral Microbiology, Associate Professor, 歯学部, 助教授 (40187133)
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| Project Period (FY) |
2003 – 2005
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| Keywords | Nickel ion / Titanium ion / Macrophage / Cell viability / Apoptosis / Superoxide dismutase / Inflammatory cytokine / DNA microarray |
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| Research Abstract |
(1)With increasing nickel ion concentrations, cell viability of macrophage-like RAW264 cells declined, and nitric oxide production of LPS-stimulated RAW 264 cells also declined. DNA microarray analyses showed that nickel ions caused cell cycle arrest, induction of apoptosis, production of inflammatory cytokines (IL-1α、IL-1α、IL-6、TNF) and increased oxidative stress, which lead to cellular damage and systematic body damage such as allergy and cancer.
(2)Cell viability of macrophage-like RAW264 cells cultured in medium with 1ppm titanium ions declined to about 55%, compared to that of control cells without titanium ions. SOD activities and TNF-α production of macrophage-like RAW264 cells cultured in medium with 1ppm titanium ions doubled, compared to those of the control cells without titanium ions. It was speculated that phagocytosis of titanium-protein complex by macrophage caused inflammatory cellular reactions.
(3)Elution of 10μm titanium particles into distilled water, MEM solution and lactic acid for 15 days was found to be negligible (0), 0.01 ppm and 9 ppm, respectively. Cell viability and NO production of macrophage-like RAW264 cells that phagocytized 10μm titanium particles were 89.4% and 91.6%, respectively. This means that macropage's cellular damage caisued by phagocytosis of 10μm titanium particles was small and tolerable.
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Research Products
(14 results)
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[Journal Article] Alloy design and property evaluation of new b-type titanium ally with excellent cold workability and biocompatibility2006
Author(s)
Taneichi, K., Taira, M., Sukedai, E., Narushima T, Iguchi, Y., Ouchi, C.
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Journal Title
ISIJ Int. 43・2
Pages: 292-301
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Alloy design and property evaluation of new b type titanium alloy with excellent cold workability and biocompatibility.2006
Author(s)
Taneichi, K., Taira, M., Sukedai, E., Narushima T., Iguchi, Y., Ouch, C.
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Journal Title
ISIJ Int. 46(2)
Pages: 292-301
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Effects of Ni^2+ ions on cell viability and NO production of murine periotoneal exudate cells (macrophages) with and without lipopolysaccharide stimulation2005
Author(s)
Taira, M., Sasaki, M., Yamaura-Tanaka, C., Saitoh, S., Kimura, S., Araki, Y.
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Journal Title
Dent. Mater. J 24・3
Pages: 304-310
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Effects of Ni^<2+> ions on cell viability and NO production of murine periotoneal exudate cells (macrophages) with and without Iipopolysaccharide stimulation.2005
Author(s)
Taira, M., Sasaki, M., Yamaura-Tanaka, C., Saitoh, S., Kimura, S., Araki, Y.
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Journal Title
Dent.Mater.J. 24(3)
Pages: 304-310
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Bio-sorption of acidic gelatin hydro-gels implanted in the back tissues of Fisher's rats.2005
Author(s)
Taira, M., Furuuchi, H., Saitoh, S., Sugiyama, Y., Sekiyama, S., Araki, Y., Tabata, Y.
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Journal Title
J.Oral Rehabil. 32
Pages: 382-387
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Microarray analysis of the cell cycle-related gene expression from a RAW264 murine macrophage cell line in response to lipopolysaccharide.2005
Author(s)
Taira, M., Sasaki, M., Kimura, S., Araki, Y.
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Journal Title
International Congress Series 1284 edited by Watanabe, N., Takahashi, N.and Takada, H., (Proceedings of the International Symposium for Interface Oral Health, held in Sendai, Japan, between 2 and 3 February 2005) Elsevier B.V.September 2005 2005
Pages: 205-206
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Bio-sorption of acidic gelatin hydro-gels implanted in the back tissues of Fisher's rats.2004
Author(s)
Taira, M., Furuuchi, H., Saitoh, S., Sugiyama, Y., Sekiyama, S., Araki, Y., Tabata, Y.
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Journal Title
J. Oral Rehabil. 32・5
Pages: 382-387
Description
「研究成果報告書概要(和文)」より
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