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2005 Fiscal Year Final Research Report Summary

Research on the pathogenic mechanism of pulmonary hypertension (PH) in mixed connective tissue disease (MCTD).

Research Project

Project/Area Number 15590312
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Human pathology
Research InstitutionIwate Medical University

Principal Investigator

SAWAI Takashi  Iwate Medical University, School of Medicine, Professor, 医学部, 教授 (00125577)

Co-Investigator(Kenkyū-buntansha) KAMATAKI Akihisa  Iwate Medical University, School of Medicine, Research Associate, 医学部, 助手 (60360004)
SASAKI Nobuhito  Iwate Medical University, School of Medicine, Research Associate, 医学部, 助手 (50382601)
Project Period (FY) 2003 – 2005
Keywordsmixed connective tissue disease (MCTD) / pulmonary hypertension / anti-endothelial cell antibody (AECA)
Research Abstract

The pathogenesis of pulmonary hypertension (PH) in mixed connective tissue disease (MCTD) is still unclear. Recent studies have suggested that PH in MCTD is resulted from endothelial cell injury. To reveal the pathogenic mechanism of PH in patients with MCTD, this research was focused on anti-endothelial cell antibodies (AECA) and identification of target proteins of AECA.
Cyto-ELISA was performed to detect AECA in MCTD patients with PH using micro vascular endothelial cell (HMVEC-L). To determine AECA prevalence, values higher than 2 S.D. above the mean of healthy control were considered as positive. Six of 9 MCTD patients had positive results. The titer of AECA was higher in MCTD patients with PH than without PH.
For identification of targets of AECA, proteins from HMVEC-L were separated by two-dimensional electrophoresis and transferred on PVDF membranes. Western blot analyses were performed with sera from healthy control or MCTD patients. Detected protein spots with a difference among the samples were selected and identified by peptide mass finger printing. About 20 protein spots were detected and half of these were identified.
As the results of two-dimensional western blot analyses, candidate antigens were obtained. These included proteins reported as autoantigen in various diseases. Furthermore, one of candidates is suggested to be associated with PH in patients with SSc. This raises possibility that the identified proteins include antigens of AECA, indicating that two-dimensional western blot analysis is useful technique for detection of the target antigens of AECA.

  • Research Products

    (11 results)

All 2005 2003

All Journal Article (10 results) Book (1 results)

  • [Journal Article] III.症状の実際-病理診断クルー-症例1 : 血管炎を伴った関節リウマチ.2005

    • Author(s)
      菅野祐幸, 澤井高志
    • Journal Title

      病理と臨床 23

      Pages: 214-217

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] III.症状の実際-病理診断クルー-症例15 : 肺高血圧症を伴った混合性結合組織病(MCTD).2005

    • Author(s)
      黒瀬顕, 浅井大志, 澤井高志
    • Journal Title

      病理と臨床 23

      Pages: 271-274

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Syoujyou no jissai - byourishindan kuru - syourei 1 : kekkanen wo tomonatta kannseturiumachi2005

    • Author(s)
      Hiroyuki Kanno, Takashi Sawai
    • Journal Title

      Pathology and Clinical Medicine 23

      Pages: 214-217

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Syoujyou no jissai - byourishindan kuru - syourei 15 : haikouketuatusyou wo tomonatta kanseturiumachi.2005

    • Author(s)
      Akira Kurose, Taishi Asai, Takashi Sawai
    • Journal Title

      Pathology and Clinical Medicine 23

      Pages: 271-274

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] 肺血管炎の概念と分類2003

    • Author(s)
      黒瀬顕, 高橋幸洋, 澤井高志
    • Journal Title

      呼吸器科 4・6

      Pages: 459-465

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Endothelial apoptosis as a possible mechanism of pulmonary hypertension in patients with mixed connective tissue disease.2003

    • Author(s)
      Kurose A, Sasaki N, Yamauchi K, Sawai T
    • Journal Title

      Arthritis Rheum. 48・9

      Pages: S620

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] 画像解析による肺動脈幹中膜の加齢学的変化.2003

    • Author(s)
      宇月美和, 岩崎真弓, 伊藤吉賢, 大内修二, 及川眞一, 澤井高志
    • Journal Title

      脈管学 43・11

      Pages: 659-666

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Pulmonary vascular - concept and classification2003

    • Author(s)
      Akira Kurose, Kouyou Takahashi, Takashi Sawai
    • Journal Title

      Respiratory Medicine 4(6)

      Pages: 459-465

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Endothelial apoptosis as a possible mechanism of pulmonary hypertension in patients with mixed connective tissue2003

    • Author(s)
      Akira Kurose, Nobuhito Sasaki, K Yamauchi
    • Journal Title

      Arthritis Rheum 48(9)

      Pages: S620

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Age-related change of media of large pulmonary artery by morphometrical2003

    • Author(s)
      Miwa Uzuki, Mayumi Iwasaki, Yoshitaka Itoh, Shuji Ouchi, Shinichi Oikawa, Takashi Sawai
    • Journal Title

      Journal Japanese College of Angiology 43(11)

      Pages: 659-666

    • Description
      「研究成果報告書概要(欧文)」より
  • [Book] 血管炎アトラス(尾崎承一、吉木敬編)2005

    • Author(s)
      高橋幸洋, 澤井高志
    • Total Pages
      97
    • Publisher
      厚生労働省難治性疾患克服研究事業 難治性血管炎に関する調査研究
    • Description
      「研究成果報告書概要(和文)」より

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Published: 2007-12-13  

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