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2004 Fiscal Year Final Research Report Summary

Analysis of Pseudomonas aeruginosa quorum sensing system using with the in vivo new biofilm model

Research Project

Project/Area Number 15591059
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 膠原病・アレルギー・感染症内科学
Research InstitutionOsaka University

Principal Investigator

TOMONO Kazunori  Osaka University, Graduate School of Medicine, Assistant Professor, 医学系研究科, 助教授 (40202204)

Co-Investigator(Kenkyū-buntansha) KOHNO Shigeru  Nagasaki University, Graduate School of Biomedical, Professor, 医薬総合研究科, 教授 (80136647)
Project Period (FY) 2003 – 2004
Keywordsrespiratory infection / Pseudomonas aeruginosa / mucosal biofilm / bacterial colonization / Quorum sensing / in vivo model
Research Abstract

We studied the role of quorum sensing system in a mouse model of chronic Pseudomonas aeruginosa infection. A chronic P.aeruginosa respiratory infection model was established by placement of a tube precoated with PAO1 (wild type), quorum sensing mutants PAO-JP1(Δlasl), PDO100(Δrhll) or PAO-JP2(Δlasl/Δrhll), in the bronchus. At day 14 after infection, the numbers of viable bacteria in the quorum sensing mutant groups were lower than in wild type group. Histopathological examination showed milder inflammatory changes in the lung of the mutant groups compared with the wild type group. The percentages of neutrophils in the bronchoalveolar lavage fluid in the quorum sensing system mutant groups were lower than in wild type group. Our findings indicate that the quorum sensing system plays an important role in chronic P.aeruginosa respiratory infection.

  • Research Products

    (5 results)

All 2005 2004

All Journal Article (5 results)

  • [Journal Article] Role of Pseudomonas aeruginosa quorum sensing system in a mouse model of chronic respiratory infection2005

    • Author(s)
      Imamura Y, Yanagihara K, Tomono K et al.
    • Journal Title

      Journal of Medical Microbiology 54

      Pages: 515-518

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Azithromycin exhibits bactericidal effects on Pseudomonau aeruginosa through interaction with the outer membrane.2005

    • Author(s)
      Imamura Y, Higasuyama Y, Tomono K., Kohno S, et al.
    • Journal Title

      Antimicrob Agents Chemother. 49

      Pages: 1377-1380

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Role of pseudomonas aeruginosa quorum sensing system in a mouse model of chronic respiratory infection.2005

    • Author(s)
      Imamura Y, Tomono K, Kohno S, et al.
    • Journal Title

      Journal of Medical Microbiology 54

      Pages: 515-518

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Quorum senseing:最近感染症の新しい抗菌ターゲットの可能性2004

    • Author(s)
      朝野和典
    • Journal Title

      日本臨床免疫学会雑誌 27

      Pages: 297-301

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Quorum sensing : The possibility of new antimicrobial target (Japanese)2004

    • Author(s)
      Tomono K
    • Journal Title

      Japanese Journalo of Clinical Immunology 27

      Pages: 297-301

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 2006-07-11  

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