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2005 Fiscal Year Final Research Report Summary

The effect of electrochemotherapy for the lungmetastasis mouse osteosarcoma cell

Research Project

Project/Area Number 15591594
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Orthopaedic surgery
Research InstitutionOsaka city university

Principal Investigator

NAKAMURA Hiroaki  Osaka city university, Orthopaedics, Assistant professor, 大学院・医学研究科, 助教授 (60227931)

Co-Investigator(Kenkyū-buntansha) GOTANI Hiroaki  Osaka city university, University lecturer, 大学院・医学研究科, 講師 (30305627)
TSUZIO Tadao  Osaka city university, Research assistant, 大学院・医学研究科, 助手 (70381994)
TAKAOKA Kunio  Osaka city university, Professor, 大学院・医学研究科, 教授 (30112048)
Project Period (FY) 2003 – 2005
Keywordsosteosarcoma / electrochemotherapy / electroporation / cancer treatment / methotrexate / drug delivery system / lung metastasis / Dunn osteosarcoma cell
Research Abstract

We clarified the effect of Electrochemotherapy (ECT) with bipolar electrode to the osteosarcoma cell with a mouse. Dunn osteosarcoma cell was transplanted in under the skin with C3H mouse, when tumor grew at 2 week later, we injected Methotrexate (MTX) 10mg/kg into the intraperitonium and executed ECT after 30 minutes. Control group, only MTX group, only electropulse group and ECT group, all divided into 4 groups, we evaluated to the tumor volume increasing rate in time-dependent. The result was shown that 40% tumor reduction in the ECT group at one time execution. Furthermore we changed the direction of electropulse to 4 or 6 directions. When the tumor size was below 5mm, tumor was exterminated but over 5mm tumor cells were locally still alive. Therefore we developed new electrode as multi needle type (9 needles). This device was inserted in the tumor directly with the gel for an electrocardiogram and ECT was done in 50V. The tumor reduced completely whether tumor size was below 5mm or not, but a skin burn was partially recognized. We lowered the voltage to 25V and executed ETC under electropulse frequency of 60 directions. The result was a better and all tumor cells were exterminated, furthermore skin burn was not recognized. Next step we examined the side effect of the ECT to a neurovascular bundle. The mouse model with the Dunn osteosarcoma infiltrated femoral AVN was used and ECT was executed under the same conditions. The result was shown that all tumor cells was death but the influence to a neurovascular bundle was little, the injury of circulation and motor function of leg was not occurred. We have established this method with a mouse model, lung metastasis model with a rabbit as a future schedule consecutively, that would examine and clarify to the curative effect.

  • Research Products

    (8 results)

All 2005 2004

All Journal Article (8 results)

  • [Journal Article] Use of local electroporation enhances methotrexate effects with minimum dose in adjuvant-induced arthritis2005

    • Author(s)
      Tada M, Inui K, Koike T, Takaoka K.
    • Journal Title

      Arthritis & Rheumatism 52

      Pages: 637-641

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Experimental spinal fusion with recombinant human bone morphogenetic Protein-2 delivered by a synthetic polymer and beta-tricalcium phosphate in a rabbit model.2005

    • Author(s)
      Namikawa T, Terai H, Suzuki E, Nakamura H, Takaoka K.
    • Journal Title

      Spine 30

      Pages: 1717-1722

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Repair of an intercalated long bone defect with a synthetic bodegradable bone-inducing implant.2005

    • Author(s)
      Yoneda M, Terai H, Imai Y, Takaoka K.
    • Journal Title

      Biomaterials 26

      Pages: 5145-5152

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Repair of an intercalated long bone defect with a synthetic biodegradable bone-inducing implant.2005

    • Author(s)
      Yoneda M, Terai H, Imai Y, Takaoka K.
    • Journal Title

      Biomaterials 26

      Pages: 5145-5152

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Experimental spinal fusion with recombinant human bone morphogenetic protein-2 delivered by a synthetic polymer and beta-tricalcium phosphate in a rabbit model.2005

    • Author(s)
      Namikawa T, Terai H, Suzuki E, Nakamura H, Takaoka K.
    • Journal Title

      Spine 30

      Pages: 1717-1722

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Does chronic cervical myelopathy affect respiratory function?2005

    • Author(s)
      Toyoda H, Nakamura H, Konishi S, Terai S, Takaoka K.
    • Journal Title

      J Neurosurg Spine 2

      Pages: 175-178

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Does chronic cervical myelopathy affect respiratory function?2004

    • Author(s)
      Toyoda H, Nakamura H, Konishi S, Terai S. Takaoka K.
    • Journal Title

      J Neurosurg Spine 2

      Pages: 175-178

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Low-Voltage Electrochemotherapy with Low-Dose Methotrexate Enhances Survival In Mice with Osteosarcoma.2004

    • Author(s)
      Isobe M, Shimizu T, Nikaido T, Takaoka K.
    • Journal Title

      Clinical Orthopaedics and Related Research 426

      Pages: 226-231

    • Description
      「研究成果報告書概要(和文)」より

URL: 

Published: 2007-12-13  

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